In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were ...In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS: Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named M1 in leiomyoma) were confirmed by Northern analysis. Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19. It was present ubiquitously in 13 tissues tested but in various levels and even in different size. L1 was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPS19 overexpression might be a universal signal in rapid cell growth tissues.展开更多
根据DNA元件百科全书计划（encyclopedia of DNA elements,ENCODE）报道显示,人类基因组中能够有效编码蛋白的基因〈2%,其余大部分为非编码基因[1]。非编码基因根据核苷酸长度分为长链非编码RNA（long noncoding RNA,LncRNA）和短链非编...根据DNA元件百科全书计划（encyclopedia of DNA elements,ENCODE）报道显示,人类基因组中能够有效编码蛋白的基因〈2%,其余大部分为非编码基因[1]。非编码基因根据核苷酸长度分为长链非编码RNA（long noncoding RNA,LncRNA）和短链非编码RNA（ncRNA、siRNA、microRNA）。展开更多
文摘In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis. RESULTS: Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named M1 in leiomyoma) were confirmed by Northern analysis. Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19. It was present ubiquitously in 13 tissues tested but in various levels and even in different size. L1 was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. No mutations have been found in human uterine leiomyomas (n=6). CONCLUSIONS: hRPS19 overexpression might be a universal signal in rapid cell growth tissues.
文摘根据DNA元件百科全书计划（encyclopedia of DNA elements,ENCODE）报道显示,人类基因组中能够有效编码蛋白的基因〈2%,其余大部分为非编码基因[1]。非编码基因根据核苷酸长度分为长链非编码RNA（long noncoding RNA,LncRNA）和短链非编码RNA（ncRNA、siRNA、microRNA）。
