Objective To investigate LC3B-Ⅱand active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy and to explore the relationship of autophagy with apoptosis in human colorectal cancer. Met...Objective To investigate LC3B-Ⅱand active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy and to explore the relationship of autophagy with apoptosis in human colorectal cancer. Methods LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues.The protein levels of LC3B-Ⅱand active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues. Results LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors,including tumor differentiation(P=0.002),growth pattern of the tumor margin (P=0.028),pN(P=0.002),pStage(P=0.032),as well as vessel and nerve plexus invasion(P=0.002).The protein level of LC3B-Ⅱin cancer tissue was significantly higher than in normal tissue(P=0.038),but the expression of active forms of procaspase-3 in cancer tissue was lower(P=0.041).There was a statistically significant positive correlation between the expression levels of LC3B-Ⅱand the active forms of procaspase-3(r=0.537,P=0.008). Conclusions Autophagy has a prosurvival role in human colorectal cancer.Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.展开更多
目的探讨黄连解毒汤醋酸乙酯提取物(ethyl acetate extract of Huanglianjiedu Decoction,EAHJD)对白色念珠菌毒力因子的作用。方法分别采用卵黄培养基法检测磷脂酶(PL)活力、牛奶平板法检测天冬氨酸蛋白酶(Sap)活力、橄榄油乳化法检测...目的探讨黄连解毒汤醋酸乙酯提取物(ethyl acetate extract of Huanglianjiedu Decoction,EAHJD)对白色念珠菌毒力因子的作用。方法分别采用卵黄培养基法检测磷脂酶(PL)活力、牛奶平板法检测天冬氨酸蛋白酶(Sap)活力、橄榄油乳化法检测脂肪酶(Lip)活力;水-烃两项测定实验检测细胞表面疏水性(CSH);RT-PCR法检测毒力因子相关基因的表达。结果 EAHJD对PL的活力无影响;1 250μg/m L EAHJD可显著抑制Sap与Lip的活力,312μg/m L EAHJD效果次之;EAHJD对CSH呈现剂量依赖性抑制,CSH1分别下调了7.69、3.57、2.95倍;分泌型酶相关基因在EAHJD作用下呈现不同倍数的变化,其中PLC1、Sap2、Sap3、Sap9、Lip3、Lip4、Lip6下调,PLB1、PLC2、Sap10、Lip5无明显变化。结论 EAHJD可抑制白色念珠菌毒力因子的活力。展开更多
基金supported by a grant from the Medical Scientific Research Foundation of Tianjin,China (No.2010KZ97)
文摘Objective To investigate LC3B-Ⅱand active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy and to explore the relationship of autophagy with apoptosis in human colorectal cancer. Methods LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues.The protein levels of LC3B-Ⅱand active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues. Results LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors,including tumor differentiation(P=0.002),growth pattern of the tumor margin (P=0.028),pN(P=0.002),pStage(P=0.032),as well as vessel and nerve plexus invasion(P=0.002).The protein level of LC3B-Ⅱin cancer tissue was significantly higher than in normal tissue(P=0.038),but the expression of active forms of procaspase-3 in cancer tissue was lower(P=0.041).There was a statistically significant positive correlation between the expression levels of LC3B-Ⅱand the active forms of procaspase-3(r=0.537,P=0.008). Conclusions Autophagy has a prosurvival role in human colorectal cancer.Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.
文摘目的探讨黄连解毒汤醋酸乙酯提取物(ethyl acetate extract of Huanglianjiedu Decoction,EAHJD)对白色念珠菌毒力因子的作用。方法分别采用卵黄培养基法检测磷脂酶(PL)活力、牛奶平板法检测天冬氨酸蛋白酶(Sap)活力、橄榄油乳化法检测脂肪酶(Lip)活力;水-烃两项测定实验检测细胞表面疏水性(CSH);RT-PCR法检测毒力因子相关基因的表达。结果 EAHJD对PL的活力无影响;1 250μg/m L EAHJD可显著抑制Sap与Lip的活力,312μg/m L EAHJD效果次之;EAHJD对CSH呈现剂量依赖性抑制,CSH1分别下调了7.69、3.57、2.95倍;分泌型酶相关基因在EAHJD作用下呈现不同倍数的变化,其中PLC1、Sap2、Sap3、Sap9、Lip3、Lip4、Lip6下调,PLB1、PLC2、Sap10、Lip5无明显变化。结论 EAHJD可抑制白色念珠菌毒力因子的活力。
