目的:观察黄芪、太子参对大鼠肾小球系膜细胞基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)及基质金属蛋白酶抑制剂-2(tissue inhibitor of metalloproteinase-2,TIMP-2)-mRNA表达的影响。探讨黄芪、太子参对肾小球硬化的防治作...目的:观察黄芪、太子参对大鼠肾小球系膜细胞基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)及基质金属蛋白酶抑制剂-2(tissue inhibitor of metalloproteinase-2,TIMP-2)-mRNA表达的影响。探讨黄芪、太子参对肾小球硬化的防治作用。方法:(1)应用血清药理学方法,制取中药的药理血清。(2)采用血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)作为刺激因子,培养大鼠肾小球系膜细胞,使其发生增殖。(3)应用反转录多聚酶链反应技术(RT-PCR)观察各组间MMP-2及TIMP-2m-RNA表达的情况。结果:黄芪、太子参对TIMP-2有显著地抑制作用,与对照组比较差异有统计学意义(P<0.05)。对MMP-2的影响差异无统计学意义。结论:黄芪、太子参通过抑制TIMP-2的基因表达,从而达到延缓肾小球硬化的作用。展开更多
OBJECTIVE： To observe effect of Liuweibuqi Capsule, a Traditional Chinese Medicine （TCM）, on the janus kinase （JAK）/signal transducer and activator of transcription （STAT） pathway and matrix metalloproteinases ...OBJECTIVE： To observe effect of Liuweibuqi Capsule, a Traditional Chinese Medicine （TCM）, on the janus kinase （JAK）/signal transducer and activator of transcription （STAT） pathway and matrix metalloproteinases （MMPs） in a chronic obstructive pulmonary disease （COPD） rat model with lung deficiency in terms of TCM＇s pattern differentiation. METHODS： Rats were randomly divided into a normal group, model group, Liuweibuqi group, Jinshuibao group, and spleen aminopeptidase group （n= 10）. Aside from the normal group, all rats were ex-posed to smoke plus lipopolysaccharide tracheal instillation to establish the COPD model with lung deficiency. Models were established after 28 days and then the normal and model groups were given normal saline （0.09 g/kg）, Liuweibuqi group was given Liuweibuqi capsule （0.35 g/kg）, Jinshuibao group was given Jinshuibao capsules （0.495 g/kg）, and the spleen group was given spleen aminopeptidase （0.33 mg/kg）, once a day for 30 days. Changes in symptoms, signs, and lung histology were observed. Lung function was measured with a spirometer. Serum cytokines were detected using enzyme-linked immunosorbent assay, and changes in the JAK/STAT pathway, MMP-9, and MMPs inhibitor 1 （TIMP1） were detected by immunohistochemistry, RT-PCR, and western blotting, respectively.RESULTS： Compared with the normal group, lung tissue was damaged, and lung function was reduced in the model control group. Additionally, the levels of interleukin （IL）-1β, y interferon （IFN-γ）, and IL-6 were higher, while IL-4 and IL-10 were lower in the model control group than those in the normal group. The expressions of JAK1, STAT3, ρ-STAT3, and MMP-9 mRNA and protein in lung tissue were higher, and TIMP1 mRNA and protein was lower in the model group compared with the normal group. After treatment, compared with the model group, the expression of inflammatory cytokines was lower in each treatment group, and expressions of JAK/ STAT pathway, MMPs were lower. Compared wi展开更多
目的:观察积雪草颗粒对TGF-β1诱导的体外培养的肾小管上皮细胞单核细胞趋化蛋白1(MCP-1)、肝细胞生长因子(HGF)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制剂-2(TIMP-2)m RNA表达的影响。方法:将体外培养的大鼠近端肾小管上皮细胞(...目的:观察积雪草颗粒对TGF-β1诱导的体外培养的肾小管上皮细胞单核细胞趋化蛋白1(MCP-1)、肝细胞生长因子(HGF)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制剂-2(TIMP-2)m RNA表达的影响。方法:将体外培养的大鼠近端肾小管上皮细胞(NRK52E)随机分为6组:正常对照组、TGF-β1刺激组、积雪草小、中、大剂量组及蒙诺组。培养48h后取出,应用实时荧光定量PCR技术检测细胞MCP-1、HGF、MMP-2、TIMP-2的m RNA表达。结果:与正常对照组比较,TGF-β1刺激组细胞MCP-1、MMP-2、TIMP-2的m RNA表达明显升高(P<0.05),HGF m RNA表达显著增加(P<0.05);各药物干预组与TGF-β1刺激组相比MCP-1、MMP-2、TIMP-2的m RNA明显下降(P<0.05),HGF m RNA表达明显增加(P<0.05);蒙诺组细胞变化与积雪草大剂量组相似。结论:积雪草抗TIF作用可能通过抑制MCP-1、MMP-2、TIMP-2的高表达,上调HGF的表达,调节MMP-2/TIMP-2的平衡而实现,且与其剂量呈正相关。展开更多
目的探究参七虫草胶囊治疗肺纤维化的机制。方法将80只雄性SD大鼠随机分成空白组、模型组、泼尼松组、参七虫草胶囊组,每组20只。采用博莱霉素气管内滴注法复制肺纤维化模型。给药后15、30d,分别观察各组大鼠肺组织病理变化,并采用免疫...目的探究参七虫草胶囊治疗肺纤维化的机制。方法将80只雄性SD大鼠随机分成空白组、模型组、泼尼松组、参七虫草胶囊组,每组20只。采用博莱霉素气管内滴注法复制肺纤维化模型。给药后15、30d,分别观察各组大鼠肺组织病理变化,并采用免疫组织化学方法检测肺组织中基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)和基质金属蛋白酶抑制剂-1(issue inhibitor of metalloproteinase-1,TIMP-1)的表达水平。结果与模型组比较,参七虫草胶囊组、泼尼松组大鼠各时点肺泡炎性反应和肺纤维化程度明显减轻,各时点泼尼松组、参七虫草胶囊组肺组织MMP-9、TIMP-1表达水平显著降低(P<0.05,或P<0.01)。结论参七虫草胶囊抑制肺纤维化早期肺泡的炎性反应与其降低肺组织MMP-9、TIMP-1表达水平有关。展开更多
文摘目的:观察黄芪、太子参对大鼠肾小球系膜细胞基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)及基质金属蛋白酶抑制剂-2(tissue inhibitor of metalloproteinase-2,TIMP-2)-mRNA表达的影响。探讨黄芪、太子参对肾小球硬化的防治作用。方法:(1)应用血清药理学方法,制取中药的药理血清。(2)采用血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)作为刺激因子,培养大鼠肾小球系膜细胞,使其发生增殖。(3)应用反转录多聚酶链反应技术(RT-PCR)观察各组间MMP-2及TIMP-2m-RNA表达的情况。结果:黄芪、太子参对TIMP-2有显著地抑制作用,与对照组比较差异有统计学意义(P<0.05)。对MMP-2的影响差异无统计学意义。结论:黄芪、太子参通过抑制TIMP-2的基因表达,从而达到延缓肾小球硬化的作用。
基金Supported by The National Natural Science Foundation Project:Study on the Metabolism of Chronic Obstructive Pulmonary Disease Pulmonary Qi Deficiency Syndrome and Cerebral Cortex Correlation Spectrum(No.81072781)
文摘OBJECTIVE： To observe effect of Liuweibuqi Capsule, a Traditional Chinese Medicine （TCM）, on the janus kinase （JAK）/signal transducer and activator of transcription （STAT） pathway and matrix metalloproteinases （MMPs） in a chronic obstructive pulmonary disease （COPD） rat model with lung deficiency in terms of TCM＇s pattern differentiation. METHODS： Rats were randomly divided into a normal group, model group, Liuweibuqi group, Jinshuibao group, and spleen aminopeptidase group （n= 10）. Aside from the normal group, all rats were ex-posed to smoke plus lipopolysaccharide tracheal instillation to establish the COPD model with lung deficiency. Models were established after 28 days and then the normal and model groups were given normal saline （0.09 g/kg）, Liuweibuqi group was given Liuweibuqi capsule （0.35 g/kg）, Jinshuibao group was given Jinshuibao capsules （0.495 g/kg）, and the spleen group was given spleen aminopeptidase （0.33 mg/kg）, once a day for 30 days. Changes in symptoms, signs, and lung histology were observed. Lung function was measured with a spirometer. Serum cytokines were detected using enzyme-linked immunosorbent assay, and changes in the JAK/STAT pathway, MMP-9, and MMPs inhibitor 1 （TIMP1） were detected by immunohistochemistry, RT-PCR, and western blotting, respectively.RESULTS： Compared with the normal group, lung tissue was damaged, and lung function was reduced in the model control group. Additionally, the levels of interleukin （IL）-1β, y interferon （IFN-γ）, and IL-6 were higher, while IL-4 and IL-10 were lower in the model control group than those in the normal group. The expressions of JAK1, STAT3, ρ-STAT3, and MMP-9 mRNA and protein in lung tissue were higher, and TIMP1 mRNA and protein was lower in the model group compared with the normal group. After treatment, compared with the model group, the expression of inflammatory cytokines was lower in each treatment group, and expressions of JAK/ STAT pathway, MMPs were lower. Compared wi
文摘目的:观察积雪草颗粒对TGF-β1诱导的体外培养的肾小管上皮细胞单核细胞趋化蛋白1(MCP-1)、肝细胞生长因子(HGF)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制剂-2(TIMP-2)m RNA表达的影响。方法:将体外培养的大鼠近端肾小管上皮细胞(NRK52E)随机分为6组:正常对照组、TGF-β1刺激组、积雪草小、中、大剂量组及蒙诺组。培养48h后取出,应用实时荧光定量PCR技术检测细胞MCP-1、HGF、MMP-2、TIMP-2的m RNA表达。结果:与正常对照组比较,TGF-β1刺激组细胞MCP-1、MMP-2、TIMP-2的m RNA表达明显升高(P<0.05),HGF m RNA表达显著增加(P<0.05);各药物干预组与TGF-β1刺激组相比MCP-1、MMP-2、TIMP-2的m RNA明显下降(P<0.05),HGF m RNA表达明显增加(P<0.05);蒙诺组细胞变化与积雪草大剂量组相似。结论:积雪草抗TIF作用可能通过抑制MCP-1、MMP-2、TIMP-2的高表达,上调HGF的表达,调节MMP-2/TIMP-2的平衡而实现,且与其剂量呈正相关。
文摘目的探究参七虫草胶囊治疗肺纤维化的机制。方法将80只雄性SD大鼠随机分成空白组、模型组、泼尼松组、参七虫草胶囊组,每组20只。采用博莱霉素气管内滴注法复制肺纤维化模型。给药后15、30d,分别观察各组大鼠肺组织病理变化,并采用免疫组织化学方法检测肺组织中基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)和基质金属蛋白酶抑制剂-1(issue inhibitor of metalloproteinase-1,TIMP-1)的表达水平。结果与模型组比较,参七虫草胶囊组、泼尼松组大鼠各时点肺泡炎性反应和肺纤维化程度明显减轻,各时点泼尼松组、参七虫草胶囊组肺组织MMP-9、TIMP-1表达水平显著降低(P<0.05,或P<0.01)。结论参七虫草胶囊抑制肺纤维化早期肺泡的炎性反应与其降低肺组织MMP-9、TIMP-1表达水平有关。
