Batocera lineolata Chevrolat,1852 is an important forest pest that is found mainly in China,Vietnam,India,and Japan.The complete mitochondrial genome(mitogenome) sequence of B.lineolata was determined by long polymera...Batocera lineolata Chevrolat,1852 is an important forest pest that is found mainly in China,Vietnam,India,and Japan.The complete mitochondrial genome(mitogenome) sequence of B.lineolata was determined by long polymerase chain reaction(PCR) and conserved primer walking approaches.The results showed that the entire mitogenome is 15418 bp long with 74.48% A+T content.The positions and arrangement of the 37 genes encoded by the genome are identical to the mitogenomes of two other longhorn beetles for which the complete gene content and arrangement are publicly available.All protein-coding genes start with the ATN codon that is a typical initiation codon in insects.All transfer RNAs(tRNAs) were predicted to form the standard clover-leaf structure,except for tRNA Ser(AGN),which lacks the dihydrouridine(DHU) arm.The most unusual feature that was found was the use of TCT as the tRNA Ser(AGN) anticodon instead of the GCT that is used in most other arthropods.The lack of tandem repeat motif in the 735 bp long A+T-rich region was another unusual feature of the B.lineolata mitogenome.The short,highly conserved polythymidine stretch that was previously described in the Orthoptera and Diptera orders was also present in the A+T-rich region of the B.lineolata(order Coleoptera) mitogenome.The sequence and annotation of the mitogenome of B.lineolata will provide further insights into the diversity and evolution of the Cerambycidae family of long-horned beetles.The B.lineolata mitogenome sequence has been deposited in GenBank(http://www.ncbi.nlm.nih.gov/Genbank/) under accession number JN986793.展开更多
[ Objective] The aim of this study was to investigate whether HSP70 can be used as a stress monitoring indicator in Cypnnus carpio breeding. [Method] Based on HSP70 sequence of Cyprinus carpio （AY120894）, one pair o...[ Objective] The aim of this study was to investigate whether HSP70 can be used as a stress monitoring indicator in Cypnnus carpio breeding. [Method] Based on HSP70 sequence of Cyprinus carpio （AY120894）, one pair of primers was designed and synthesized, while the total RNA of liver tissues in Cyprinus carpio was extracted. Some cDNA fragments of Cyprinus carpio HSP70 were cloned by RT-PCR, and its differential expression in various tissues such as heart, intestine, mucus, gonad, swim bladder, gill and fin in Cyprinus carpio was also studied. [Result] The cDNA sequence of 480 bp was obtained from Cypdnus carpio HSPTO gene by RT-PCR amplification. Homology comparison between the deduced amino acid sequence after sequencing and that of other types of fish showed that the homology among Cyprinus carpio, Danio rerio, Ohcorhynehus mylciss, Paralichthys olivaceus, Xiphophoorus maculates and Carassius auratus was 96%, 98%, 98%, 96%, 98% and 96% respectively. The expression of HSP70 was detected in eight tissues of Cypnnus carpio. The expression was the highest in heart, followed by swim bladder and fin, but there was no significant difference between them （ P 〉 0.05 ）. There was no significant difference among the ex- pression in three tissues of intestine, mucus and fat （ P〉0.05）, but their expression was significantly higher than those in gonad and gill （ P〈 0.05）. [ Conclusion] HSPTO gene expression is a suitable criterion for monitoring the stress degree, stress capacity and healthy conditions in Cyprinus carpio breeding.展开更多
[ Objective] To identify a novel snoRNA gene in Arabidopsis thalianan. [ Method ] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form an...[ Objective] To identify a novel snoRNA gene in Arabidopsis thalianan. [ Method ] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form and function of typical candidate gene were analyzed. [ Result] The identified snR95 box H/ACA snoRNA had conservative component and structural features of box C/D snoRNA family, possessed two more than 10 nt long rRNA antisense elements. The result revealed that the novel snoRNA is a partial counterpart of the rice Z270, named box C/D snoRNA -AthZ270. [ Conclusion ] Z270 snoRNA in Arabidopsis thalianan has different function with common snoRNA.展开更多
基金supported by the National Ministry of Forestry of China (2009-4-37)the Science and Technology Committee of Yunnan Province,China (2011FB141)
文摘Batocera lineolata Chevrolat,1852 is an important forest pest that is found mainly in China,Vietnam,India,and Japan.The complete mitochondrial genome(mitogenome) sequence of B.lineolata was determined by long polymerase chain reaction(PCR) and conserved primer walking approaches.The results showed that the entire mitogenome is 15418 bp long with 74.48% A+T content.The positions and arrangement of the 37 genes encoded by the genome are identical to the mitogenomes of two other longhorn beetles for which the complete gene content and arrangement are publicly available.All protein-coding genes start with the ATN codon that is a typical initiation codon in insects.All transfer RNAs(tRNAs) were predicted to form the standard clover-leaf structure,except for tRNA Ser(AGN),which lacks the dihydrouridine(DHU) arm.The most unusual feature that was found was the use of TCT as the tRNA Ser(AGN) anticodon instead of the GCT that is used in most other arthropods.The lack of tandem repeat motif in the 735 bp long A+T-rich region was another unusual feature of the B.lineolata mitogenome.The short,highly conserved polythymidine stretch that was previously described in the Orthoptera and Diptera orders was also present in the A+T-rich region of the B.lineolata(order Coleoptera) mitogenome.The sequence and annotation of the mitogenome of B.lineolata will provide further insights into the diversity and evolution of the Cerambycidae family of long-horned beetles.The B.lineolata mitogenome sequence has been deposited in GenBank(http://www.ncbi.nlm.nih.gov/Genbank/) under accession number JN986793.
文摘[ Objective] The aim of this study was to investigate whether HSP70 can be used as a stress monitoring indicator in Cypnnus carpio breeding. [Method] Based on HSP70 sequence of Cyprinus carpio （AY120894）, one pair of primers was designed and synthesized, while the total RNA of liver tissues in Cyprinus carpio was extracted. Some cDNA fragments of Cyprinus carpio HSP70 were cloned by RT-PCR, and its differential expression in various tissues such as heart, intestine, mucus, gonad, swim bladder, gill and fin in Cyprinus carpio was also studied. [Result] The cDNA sequence of 480 bp was obtained from Cypdnus carpio HSPTO gene by RT-PCR amplification. Homology comparison between the deduced amino acid sequence after sequencing and that of other types of fish showed that the homology among Cyprinus carpio, Danio rerio, Ohcorhynehus mylciss, Paralichthys olivaceus, Xiphophoorus maculates and Carassius auratus was 96%, 98%, 98%, 96%, 98% and 96% respectively. The expression of HSP70 was detected in eight tissues of Cypnnus carpio. The expression was the highest in heart, followed by swim bladder and fin, but there was no significant difference between them （ P 〉 0.05 ）. There was no significant difference among the ex- pression in three tissues of intestine, mucus and fat （ P〉0.05）, but their expression was significantly higher than those in gonad and gill （ P〈 0.05）. [ Conclusion] HSPTO gene expression is a suitable criterion for monitoring the stress degree, stress capacity and healthy conditions in Cyprinus carpio breeding.
基金Supported by Natural Science Project Establishment Subject of Jiangxi Science & Technology Normal University(KY2008ZY03 )Teaching Research Provincial Project Establishment Subject of Higher Education of Jiangxi Province (JXJG-13-29)~~
文摘[ Objective] To identify a novel snoRNA gene in Arabidopsis thalianan. [ Method ] Genome sequence of Arabidopsis thalianan was screened by using bioinformatics methods, and the sequence structure, organization form and function of typical candidate gene were analyzed. [ Result] The identified snR95 box H/ACA snoRNA had conservative component and structural features of box C/D snoRNA family, possessed two more than 10 nt long rRNA antisense elements. The result revealed that the novel snoRNA is a partial counterpart of the rice Z270, named box C/D snoRNA -AthZ270. [ Conclusion ] Z270 snoRNA in Arabidopsis thalianan has different function with common snoRNA.
