AIM:To investigate the influence of autologous cytokine- induced killer (CIK) cells on the phenotypes of CIK effector cells,peripheral T lymphocyte subsets and dendritic cell subsets in patients with primary hepatocel...AIM:To investigate the influence of autologous cytokine- induced killer (CIK) cells on the phenotypes of CIK effector cells,peripheral T lymphocyte subsets and dendritic cell subsets in patients with primary hepatocellular carcinoma (HCC). METHODS:Peripheral blood mononuclear cells (PBMC) were collected by a blood cell separator from 13 patients with HCC,then expanded by priming them with interferon- gamma (IFN-γ) followed by monoclonal antibody (mAb) against CD3 and interleukin-2 (IL-2) the next day.The phenotypic patterns of CIK cells were characterized by flow cytometry on d 0,4,7,10,13 and 15 of incubation, respectively.Then,5 mL of venous blood was obtained from HCC patients before or 8-10 d after CIK cells were transfused into patients to assess the influence of CIK cells on the percentages of effector cells,and proportions of DC1 or DC2 in peripheral blood by flow cytometry. RESULTS:After two weeks of in vitro incubation,the percentages of CD3^+CD8^+,CD3^+CD56^+,and CD25^+ cells increased significantly from 33.5±10.1%,7.7±2.8%,and 12.3±4.5% to 36.6±9.0% (P<0.05),18.9±6.9% (P<0.01), and 16.4±5.9% (P<0.05),respectively.However,the percentages of CD3^+CD4^+ and NK cells had no significant difference.The percentages of CD3^+ and CD3^+CD8^+ cells were kept at high levels during the whole incubation period,but those of CD25^+,and CD3^+CD56^+ cells began to decrease on d 7 and 13,respectively.The proportions of type Ⅰ dendritic cell (DC1) and type Ⅱ dendritic cell (DC2) subsets increased from 0.59±0.23% and 0.26±0.12% before CIK cell therapy to 0.85±0.27% and 0.43±0.19% (all P<0.01) after CIK cell transfusion,respectively.The symptoms and characteristics of HCC patients were relieved without major side effects. CONCLUSION:Our results indicated that autologous CIK cells can efficiently improve the immunological status in HCC patients,and may provide a potent approach for HCC patients as the adoptive immunotherapy.展开更多
树突状细胞(Dendritic cells, DC)的细胞与分子生物学Keystone会议(Keystone Symposium on Cellular and Molecular Biology of Dendritic Cells)于1998年3月7日～13日于美国Santa Fe召开,来自不同国家的300余名学者参加了会议。
文摘AIM:To investigate the influence of autologous cytokine- induced killer (CIK) cells on the phenotypes of CIK effector cells,peripheral T lymphocyte subsets and dendritic cell subsets in patients with primary hepatocellular carcinoma (HCC). METHODS:Peripheral blood mononuclear cells (PBMC) were collected by a blood cell separator from 13 patients with HCC,then expanded by priming them with interferon- gamma (IFN-γ) followed by monoclonal antibody (mAb) against CD3 and interleukin-2 (IL-2) the next day.The phenotypic patterns of CIK cells were characterized by flow cytometry on d 0,4,7,10,13 and 15 of incubation, respectively.Then,5 mL of venous blood was obtained from HCC patients before or 8-10 d after CIK cells were transfused into patients to assess the influence of CIK cells on the percentages of effector cells,and proportions of DC1 or DC2 in peripheral blood by flow cytometry. RESULTS:After two weeks of in vitro incubation,the percentages of CD3^+CD8^+,CD3^+CD56^+,and CD25^+ cells increased significantly from 33.5±10.1%,7.7±2.8%,and 12.3±4.5% to 36.6±9.0% (P<0.05),18.9±6.9% (P<0.01), and 16.4±5.9% (P<0.05),respectively.However,the percentages of CD3^+CD4^+ and NK cells had no significant difference.The percentages of CD3^+ and CD3^+CD8^+ cells were kept at high levels during the whole incubation period,but those of CD25^+,and CD3^+CD56^+ cells began to decrease on d 7 and 13,respectively.The proportions of type Ⅰ dendritic cell (DC1) and type Ⅱ dendritic cell (DC2) subsets increased from 0.59±0.23% and 0.26±0.12% before CIK cell therapy to 0.85±0.27% and 0.43±0.19% (all P<0.01) after CIK cell transfusion,respectively.The symptoms and characteristics of HCC patients were relieved without major side effects. CONCLUSION:Our results indicated that autologous CIK cells can efficiently improve the immunological status in HCC patients,and may provide a potent approach for HCC patients as the adoptive immunotherapy.
文摘树突状细胞(Dendritic cells, DC)的细胞与分子生物学Keystone会议(Keystone Symposium on Cellular and Molecular Biology of Dendritic Cells)于1998年3月7日～13日于美国Santa Fe召开,来自不同国家的300余名学者参加了会议。
