An epidemic of hepatitis in the south part of Xinjiang Uighur Autonomous Reg ion during 1986-1988, and 2 548 acute sporadic hepatitis cases from 17 cities of Ch i na were studied. The disease had following clinical an...An epidemic of hepatitis in the south part of Xinjiang Uighur Autonomous Reg ion during 1986-1988, and 2 548 acute sporadic hepatitis cases from 17 cities of Ch i na were studied. The disease had following clinical and epidemiological char acte ristics: 86.4% cases occurred in the age group of 20-59 years; with male prepon d erance and autumn-winter excess; 75% patients had a history of contact with hep a titis cases, and / or eating out or drinking unboiled water; clinical features w ere s imilar to hepatitis A, with a higher fatality rate, especially in pregnant women (up to 21%). Two strains of hepatitis E virus (HEV) isolated from Xinjiang were completely seq uenced by dideoxy chain termina tion method, with nucleotide homology of 93.5% and 94.5% with Burma strain (geno type 1), 75.7% and 75.9% with Mexico strain (genotype 2), 73.7% and 74.2 % with American strain (genotype 3), respectively. It suggests that these two st rains belong to genotype 1. The partial sequencing of open reading frame (ORF)2 region of 98 HEV isol ates from 19 cities of China revealed that 62 of them (63.3%) shared the same ge notype with Burma and Xinjiang strains, and 36 (36.7%) isolates were of genotype 4. One of the 36 strains was completely sequenced, with nucleotide identity of 75% with Burma strain, 74.5% with Mexico strain and 75.3% with American strain , respectively. Sera collected from 419 pigs, 190 cattle and 316 goats from var ious regions of China were detected for anti-HEV antibodies and HEV RNA using a n in-house enzyme immunoassay (EIA) and reverse transcriptase nested polymerase chain re action (RT-nPCR) with primers from ORF 2. The mean positivity rates of anti-HEV antibody for pigs and cattle were 78.8% and 6. 3%, respectively, but none of the goat sera was anti-HEV positive. The PCR product s (nt 6007- 6354) of 5 HEV RNA positive sera were sequenced and compared to othe r HEV sequences in the nucleotide databases. The five sequences shared 74%-79%, 73%-74%, 73%-78%, and 83%-99% identity to HEV genotypes 1,2,3 and 4展开更多
The purpose of the present study was to determine the prevalence of swine Hepatitis E virus (HEV) infection in Xinjiang. 813 swine serum samples collected from 1 to 12 months of age at 9 swine farms in Xinjiang region...The purpose of the present study was to determine the prevalence of swine Hepatitis E virus (HEV) infection in Xinjiang. 813 swine serum samples collected from 1 to 12 months of age at 9 swine farms in Xinjiang region were tested by ELISA for the presence of IgG antibodies against HEV. The recombinant protein pUS166 containing region 452-617aa of the ORF2 of HEV US strain was used as coating antigen. The result showed that anti –HEV IgG were detected in 265 of 405 pigs (65.43%) in one group and 238 of 408 pigs (58.33%) in another group , and that the seropositivity rate was not related to geographic district and breeds, but differed remarkably by age, being 40% among the 1- to 3-month-old piglets, but 77.33% among ones over 3-month-old. It suggested that swine HEV was widespread in different geographic regions of XinJiang.展开更多
文摘目的探讨乙型肝炎表面抗原（HBsAg）对急性甲型肝炎严重性的影响。方法回顾性分析2008年1月-2010年12月449名急性甲型肝炎住院患者的临床特征,比较其临床结果,探讨HBsAg对其预后的影响。结果 449例患者中,30例患者呈现HBsAg阳性组,419例HBsAg阴性。HBsAg阳性组年龄大于HBsAg阴性组（36.1±8.3 vs 31.8±8.5）岁,P=0.004）,两组间其他基线特征无差异。HBsAg阳性组的凝血酶原时间达峰平均值、血清总胆红素、血清肌酐显著明显高于HBsAg阴性组。胃肠道出血,急性肾功能衰竭,急性肝功能衰竭等临床结果在HBsAg阳性组中出现频率更高,尤其是急性肝功能衰竭发病率约为HBsAg阴性组的9倍（23.3%vs 3.3%;[OR],8.80,P〈0.001）。多因素分析表明乙肝表面抗原（OR,7.43;95%置信度置信区间[CI],2.56-21.57）和年龄（OR,1.07,95%CI,1.02-1.13）是急性肝衰竭的发生的两个独立因素。结论与单纯急性甲型肝炎患者相比,合并慢性乙型肝炎感染的急性甲型肝炎患者有更严重的临床症状。
文摘An epidemic of hepatitis in the south part of Xinjiang Uighur Autonomous Reg ion during 1986-1988, and 2 548 acute sporadic hepatitis cases from 17 cities of Ch i na were studied. The disease had following clinical and epidemiological char acte ristics: 86.4% cases occurred in the age group of 20-59 years; with male prepon d erance and autumn-winter excess; 75% patients had a history of contact with hep a titis cases, and / or eating out or drinking unboiled water; clinical features w ere s imilar to hepatitis A, with a higher fatality rate, especially in pregnant women (up to 21%). Two strains of hepatitis E virus (HEV) isolated from Xinjiang were completely seq uenced by dideoxy chain termina tion method, with nucleotide homology of 93.5% and 94.5% with Burma strain (geno type 1), 75.7% and 75.9% with Mexico strain (genotype 2), 73.7% and 74.2 % with American strain (genotype 3), respectively. It suggests that these two st rains belong to genotype 1. The partial sequencing of open reading frame (ORF)2 region of 98 HEV isol ates from 19 cities of China revealed that 62 of them (63.3%) shared the same ge notype with Burma and Xinjiang strains, and 36 (36.7%) isolates were of genotype 4. One of the 36 strains was completely sequenced, with nucleotide identity of 75% with Burma strain, 74.5% with Mexico strain and 75.3% with American strain , respectively. Sera collected from 419 pigs, 190 cattle and 316 goats from var ious regions of China were detected for anti-HEV antibodies and HEV RNA using a n in-house enzyme immunoassay (EIA) and reverse transcriptase nested polymerase chain re action (RT-nPCR) with primers from ORF 2. The mean positivity rates of anti-HEV antibody for pigs and cattle were 78.8% and 6. 3%, respectively, but none of the goat sera was anti-HEV positive. The PCR product s (nt 6007- 6354) of 5 HEV RNA positive sera were sequenced and compared to othe r HEV sequences in the nucleotide databases. The five sequences shared 74%-79%, 73%-74%, 73%-78%, and 83%-99% identity to HEV genotypes 1,2,3 and 4
文摘The purpose of the present study was to determine the prevalence of swine Hepatitis E virus (HEV) infection in Xinjiang. 813 swine serum samples collected from 1 to 12 months of age at 9 swine farms in Xinjiang region were tested by ELISA for the presence of IgG antibodies against HEV. The recombinant protein pUS166 containing region 452-617aa of the ORF2 of HEV US strain was used as coating antigen. The result showed that anti –HEV IgG were detected in 265 of 405 pigs (65.43%) in one group and 238 of 408 pigs (58.33%) in another group , and that the seropositivity rate was not related to geographic district and breeds, but differed remarkably by age, being 40% among the 1- to 3-month-old piglets, but 77.33% among ones over 3-month-old. It suggested that swine HEV was widespread in different geographic regions of XinJiang.
