Dear Editor, An increasing number of single-nucleotide polymorphisms （SNP） have been identified and extensively used in genetics studies with advances in next-generation sequencing （NGS） and SNP arrays. However, d...Dear Editor, An increasing number of single-nucleotide polymorphisms （SNP） have been identified and extensively used in genetics studies with advances in next-generation sequencing （NGS） and SNP arrays. However, due to frequent calling errors, the accuracy of SNPs of interest must usually be validated. In recent years, the single-nucleotide polymorphism genotyp-ing by fluorescent competitive allele-specific polymerase chain reaction （NiPTag, the commercial technology named KASP）, a relatively new and powerful SNP genotyping technique, relies on a mismatched nucleotide of a primer, and the mismatched nucleotide prevents amplification of the mutated allele.展开更多
基金supported by the State’s Key Project of Research & Development Plan for Breeding of Top-seven Crops (2017YFD0100801)the National Natural Science Foundation of China (31600997, 31771772)+1 种基金the Yangzhou Key Research and Development Program (YZ2016035)the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
文摘Dear Editor, An increasing number of single-nucleotide polymorphisms （SNP） have been identified and extensively used in genetics studies with advances in next-generation sequencing （NGS） and SNP arrays. However, due to frequent calling errors, the accuracy of SNPs of interest must usually be validated. In recent years, the single-nucleotide polymorphism genotyp-ing by fluorescent competitive allele-specific polymerase chain reaction （NiPTag, the commercial technology named KASP）, a relatively new and powerful SNP genotyping technique, relies on a mismatched nucleotide of a primer, and the mismatched nucleotide prevents amplification of the mutated allele.
