Aquaporin 9 (AQP9) is one of the recently identified water channels that is al so permeable to neutral solutes including urea. To investigate the molecular mec hanism of intramembranous pathway of amniotic fluid regul...Aquaporin 9 (AQP9) is one of the recently identified water channels that is al so permeable to neutral solutes including urea. To investigate the molecular mec hanism of intramembranous pathway of amniotic fluid regulation, we sought to det ermine whether AQP9 is expressed, and the cellular localization of AQP9 expressi on in human fetal membranes. Fetal membranes from 5 normal term human pregnancie s were studied. Northern analysis was used to determine the tissue AQP9 messenge r RNA (mRNA) expression. In situ hybridization and immunohistochemical staining with specific anti-AQP9 antibody was used for cellular AQP9 localization in the human fetal membranes. Northern analysis detected AQP9 mRNA expression in human amnion, chorion, and placenta. In situ hybridization revealed AQP9 mRNA express ion in epithelial cells of the amnion, chorion cytotrophoblasts, and syncytiotro phoblasts and cytotrophoblasts of placenta. Further immunohistochemical study co nfirmed the AQP9 protein expression in these cell types of fetal membranes. This study demonstrated the expression of AQP9 mRNA and protein in human chorioamnio tic membranes and placenta. The AQP9 expression in fetal membranes suggests that AQP9 may be an important water channel in intramembranous amniotic fluid water regulation.展开更多
美国华盛顿哥伦比亚特区的科学家指出,一种抗粘连疫苗能保护所有妊娠妇女避免患疟疾。恶性疟原虫粘附在合胞体滋养层表面的硫酸软骨素A(CSA)上,并且隐藏在胎盘内。因此,可使妊娠妇女处在疟疾感染的高危状态。 Michal Fried及其同事对肯...美国华盛顿哥伦比亚特区的科学家指出,一种抗粘连疫苗能保护所有妊娠妇女避免患疟疾。恶性疟原虫粘附在合胞体滋养层表面的硫酸软骨素A(CSA)上,并且隐藏在胎盘内。因此,可使妊娠妇女处在疟疾感染的高危状态。 Michal Fried及其同事对肯尼亚一个地方病地区中的居民血清寄生虫进行了孵育培养,然后测定与CSA结合的寄生虫。男性和刚怀孕妇女的血清不能抑制寄生虫结合。展开更多
The present investigation has been carried out by using the technique of RIA to analyse the effect of sodium dchydroandrographolide succinate ( Chuan IV ) on the secretion of human chorionic gonadotropin (hCG) ofchori...The present investigation has been carried out by using the technique of RIA to analyse the effect of sodium dchydroandrographolide succinate ( Chuan IV ) on the secretion of human chorionic gonadotropin (hCG) ofchorionic villi tissue in culture. By the applica-展开更多
A radioimmunoasay of high sendtivity end smbility was developed For placental proteinPP5 (PP5), a syncytiotrophoblast product oF the human placenta. We measured 94 samples from 17 normal nonpregnant women, 47 normal p...A radioimmunoasay of high sendtivity end smbility was developed For placental proteinPP5 (PP5), a syncytiotrophoblast product oF the human placenta. We measured 94 samples from 17 normal nonpregnant women, 47 normal pregnant women, and 30展开更多
文摘Aquaporin 9 (AQP9) is one of the recently identified water channels that is al so permeable to neutral solutes including urea. To investigate the molecular mec hanism of intramembranous pathway of amniotic fluid regulation, we sought to det ermine whether AQP9 is expressed, and the cellular localization of AQP9 expressi on in human fetal membranes. Fetal membranes from 5 normal term human pregnancie s were studied. Northern analysis was used to determine the tissue AQP9 messenge r RNA (mRNA) expression. In situ hybridization and immunohistochemical staining with specific anti-AQP9 antibody was used for cellular AQP9 localization in the human fetal membranes. Northern analysis detected AQP9 mRNA expression in human amnion, chorion, and placenta. In situ hybridization revealed AQP9 mRNA express ion in epithelial cells of the amnion, chorion cytotrophoblasts, and syncytiotro phoblasts and cytotrophoblasts of placenta. Further immunohistochemical study co nfirmed the AQP9 protein expression in these cell types of fetal membranes. This study demonstrated the expression of AQP9 mRNA and protein in human chorioamnio tic membranes and placenta. The AQP9 expression in fetal membranes suggests that AQP9 may be an important water channel in intramembranous amniotic fluid water regulation.
文摘美国华盛顿哥伦比亚特区的科学家指出,一种抗粘连疫苗能保护所有妊娠妇女避免患疟疾。恶性疟原虫粘附在合胞体滋养层表面的硫酸软骨素A(CSA)上,并且隐藏在胎盘内。因此,可使妊娠妇女处在疟疾感染的高危状态。 Michal Fried及其同事对肯尼亚一个地方病地区中的居民血清寄生虫进行了孵育培养,然后测定与CSA结合的寄生虫。男性和刚怀孕妇女的血清不能抑制寄生虫结合。
文摘The present investigation has been carried out by using the technique of RIA to analyse the effect of sodium dchydroandrographolide succinate ( Chuan IV ) on the secretion of human chorionic gonadotropin (hCG) ofchorionic villi tissue in culture. By the applica-
文摘A radioimmunoasay of high sendtivity end smbility was developed For placental proteinPP5 (PP5), a syncytiotrophoblast product oF the human placenta. We measured 94 samples from 17 normal nonpregnant women, 47 normal pregnant women, and 30
