目的探讨叶酸受体(FR)阳性循环肿瘤细胞(CTC)检测对肺癌的诊断效能。方法胸部CT检查提示可疑肺癌的孤立性肺小结节患者38例,术后病理诊断为早期肺癌29例、肺良性病变9例。术前均留取静脉血检测FR阳性CTC。结果 29例早期肺癌及9例肺良性...目的探讨叶酸受体(FR)阳性循环肿瘤细胞(CTC)检测对肺癌的诊断效能。方法胸部CT检查提示可疑肺癌的孤立性肺小结节患者38例,术后病理诊断为早期肺癌29例、肺良性病变9例。术前均留取静脉血检测FR阳性CTC。结果 29例早期肺癌及9例肺良性病变患者的FR阳性CTC分别为(10.87±2.62)、(8.03±2.56)FU/3 m L,两者相比,P<0.05。以术后病理检查结果作为诊断肺癌的金标准,kappa一致性检验显示FR阳性CTC诊断肺结节具有一定的准确性(kappa=0.649,P<0.01)。以FR阳性CTC作为肺癌的诊断指标,其ROC曲线下面积为0.837(95%CI为0.650~1.000),最佳截断值为8.7 FU/3 m L,诊断灵敏度为89.7%、特异度为77.8%。结论FR阳性CTC检测对早期肺癌具有较好的诊断效能。展开更多
Anion starch nanoparticles (StNP) were prepared in water-in-oil microemulsion. Folate modi-fied with PEG was conjugated to the surface of StNP to obtain the folate-conjugated starch nanoparticles (FA-PEG/StNP). The av...Anion starch nanoparticles (StNP) were prepared in water-in-oil microemulsion. Folate modi-fied with PEG was conjugated to the surface of StNP to obtain the folate-conjugated starch nanoparticles (FA-PEG/StNP). The average diameter of FA-PEG/ StNP determined by AFM and Zeta-Sizer apparatus was about 130 nm. Doxorubicin (DOX)-loaded FA- PEG/StNP was obtained via infiltrating combination. The result of UV spectrophotometer showed that the saturation concentration of DOX-loaded FA-PEG/ StNP was 28 μg/mg, which was effective for the con-trolled release of anticancer drug DOX. The cell ex-periments showed that the Lc50 of DOX-loaded FA-PEG/StNP and DOX-loaded StNP was higher than that of DOX, which indicates that FA-PEG/StNP and StNP can decrease the toxicity of DOX; while the lethal rate of DOX-loaded FA-PEG/StNP was 3 times that of DOX-loaded StNP with the same quantity of DOX, which indicates that FA in FA-PEG/StNP is ef-fective for improving the targeting function of nanoparticles, thus enhancing the inhibition effect of anticancer drug to cancer cell. This work demon-strates that the FA-PEG/StNP system is a potentially useful system for the targeted delivery of anticancer drug DOX.展开更多
文摘目的探讨叶酸受体(FR)阳性循环肿瘤细胞(CTC)检测对肺癌的诊断效能。方法胸部CT检查提示可疑肺癌的孤立性肺小结节患者38例,术后病理诊断为早期肺癌29例、肺良性病变9例。术前均留取静脉血检测FR阳性CTC。结果 29例早期肺癌及9例肺良性病变患者的FR阳性CTC分别为(10.87±2.62)、(8.03±2.56)FU/3 m L,两者相比,P<0.05。以术后病理检查结果作为诊断肺癌的金标准,kappa一致性检验显示FR阳性CTC诊断肺结节具有一定的准确性(kappa=0.649,P<0.01)。以FR阳性CTC作为肺癌的诊断指标,其ROC曲线下面积为0.837(95%CI为0.650~1.000),最佳截断值为8.7 FU/3 m L,诊断灵敏度为89.7%、特异度为77.8%。结论FR阳性CTC检测对早期肺癌具有较好的诊断效能。
文摘Anion starch nanoparticles (StNP) were prepared in water-in-oil microemulsion. Folate modi-fied with PEG was conjugated to the surface of StNP to obtain the folate-conjugated starch nanoparticles (FA-PEG/StNP). The average diameter of FA-PEG/ StNP determined by AFM and Zeta-Sizer apparatus was about 130 nm. Doxorubicin (DOX)-loaded FA- PEG/StNP was obtained via infiltrating combination. The result of UV spectrophotometer showed that the saturation concentration of DOX-loaded FA-PEG/ StNP was 28 μg/mg, which was effective for the con-trolled release of anticancer drug DOX. The cell ex-periments showed that the Lc50 of DOX-loaded FA-PEG/StNP and DOX-loaded StNP was higher than that of DOX, which indicates that FA-PEG/StNP and StNP can decrease the toxicity of DOX; while the lethal rate of DOX-loaded FA-PEG/StNP was 3 times that of DOX-loaded StNP with the same quantity of DOX, which indicates that FA in FA-PEG/StNP is ef-fective for improving the targeting function of nanoparticles, thus enhancing the inhibition effect of anticancer drug to cancer cell. This work demon-strates that the FA-PEG/StNP system is a potentially useful system for the targeted delivery of anticancer drug DOX.
