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HPLC法测定红曲霉发酵样品中Monacolin K的含量 被引量:17
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作者 贾波 孙佰申 周立平 《食品与发酵工业》 CAS CSCD 北大核心 2003年第1期70-72,82,共4页
利用HPLC法对红曲霉发酵样品中MonacolinK的测定方法进行了研究。以V (甲醇 )∶V(0 18%磷酸 ) =77∶2 3为流动相 ,流速 0 6mL/min ,检测波长 2 3 7nm ,用外标法进行定量 ,相对标准偏差和平均回收率分别为 0 .2 7%和 98 92 %。用甲醇... 利用HPLC法对红曲霉发酵样品中MonacolinK的测定方法进行了研究。以V (甲醇 )∶V(0 18%磷酸 ) =77∶2 3为流动相 ,流速 0 6mL/min ,检测波长 2 3 7nm ,用外标法进行定量 ,相对标准偏差和平均回收率分别为 0 .2 7%和 98 92 %。用甲醇提取红曲米样品 ,摇床振荡 (3 0℃ ,2 0 0r/min) 2 5~ 3h可将MonacolinK提取完全。红曲霉摇瓶发酵产生的MonacolinK有 5 0 %以上存在于发酵滤液中 ,因此可以直接取滤液进行分析。该方法简便有效 。 展开更多
关键词 HPLC法 测定 红曲霉 发酵样品 MonacolinK含量
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HPLC法测定红曲霉发酵样品中麦角固醇的含量 被引量:16
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作者 孙佰申 周立平 +1 位作者 陈旭峰 马莉锋 《中国食品添加剂》 CAS 2004年第2期89-92,共4页
采用HPLC法对红曲霉发酵样品中的麦角固醇 (Ergosterol)进行测定。色谱柱为Shim_packVP -ODSC18(4.6× 15 0mm ;5 μm)柱 ,以甲醇—水 (97:3)作为流动相 ,流速1.8ml/min ,紫外检测波长 2 82nm。红曲霉发酵样品经碱性乙醇皂化、乙醚... 采用HPLC法对红曲霉发酵样品中的麦角固醇 (Ergosterol)进行测定。色谱柱为Shim_packVP -ODSC18(4.6× 15 0mm ;5 μm)柱 ,以甲醇—水 (97:3)作为流动相 ,流速1.8ml/min ,紫外检测波长 2 82nm。红曲霉发酵样品经碱性乙醇皂化、乙醚萃取后 ,用外标法进行定量检测。结果表明 :Ergosterol标准曲线在 0 .0 2mg/ml~ 1.6mg/ml范围内呈线性关系 ,r =0 .994 9,该法的回收率为 98.86 % ,相对标准误差为 0 .15 %。本测定方法操作简便、准确、可靠。 展开更多
关键词 HPLC法 红曲霉 发酵样品 麦角固醇 测定方法
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流动注射分析(FIA)-乙醇生物电极法快速测定发酵样品中的乙醇含量 被引量:5
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作者 张维成 何亚明 +1 位作者 吕跃钢 尚艳娥 《分析测试学报》 CAS CSCD 1998年第4期22-25,共4页
报道了用流动注射分析(FIA)-乙醇生物电极法快速测定发酵样品中的乙醇含量,探讨了发酵样品中物质对测定的干扰情况,并对该法的可靠性和重现性进行了研究。用直接比较法测定1713×10-4mol/L乙醇标样,相对标... 报道了用流动注射分析(FIA)-乙醇生物电极法快速测定发酵样品中的乙醇含量,探讨了发酵样品中物质对测定的干扰情况,并对该法的可靠性和重现性进行了研究。用直接比较法测定1713×10-4mol/L乙醇标样,相对标准偏差(RSD)和相对误差(RE)分别为13%和03%(n=6)。对浓度为4150×10-4mol/L发酵样品的稀释样的测定,其RSD为05%(n=6),加标回收率为917%~1034%。根据对啤酒成品样、啤酒发酵样和酒精发酵成熟醪中的乙醇所进行的为期7天的现场对照测定结果,表明该法选择性好、快速、较准确、可满足工厂对乙醇监测的需要,便于实现操作的连续化和自动化。 展开更多
关键词 乙醇生物电极 乙醇 发酵样品 测定 FIA 传感器
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德氏乳杆菌保加利亚亚种发酵对牛乳中β-酪蛋白抗原性的影响 被引量:2
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作者 廖萍 罗永康 +1 位作者 李铮 刘晓宇 《中国乳品工业》 CAS 北大核心 2012年第3期11-14,共4页
复原脱脂牛乳经德氏乳杆菌保加利亚亚种发酵至凝乳,然后在4℃下冷藏5 d。用间接竞争ELISA法测定了其中β-酪蛋白(β-CN)的抗原残留量,并且对发酵样品的蛋白水解程度与滴定酸度进行了分析。结果表明,复原脱脂乳经90~95℃处理5 min后,β... 复原脱脂牛乳经德氏乳杆菌保加利亚亚种发酵至凝乳,然后在4℃下冷藏5 d。用间接竞争ELISA法测定了其中β-酪蛋白(β-CN)的抗原残留量,并且对发酵样品的蛋白水解程度与滴定酸度进行了分析。结果表明,复原脱脂乳经90~95℃处理5 min后,β-CN的抗原残留量为60.25%,发酵过程中其抗原残留量持续下降,凝乳时下降到55.46%,而冷藏1 d后降到了最低值54.03%,随后又缓慢上升,冷藏3 d后为55.87%,继续冷藏至5 d,β-CN的抗原残留量基本没有变化,维持在55.5%与55.9%之间。因此采用德氏乳杆菌保加利亚亚种发酵能够在一定程度上降低牛乳中β-CN的抗原性,但是在冷藏5 d的过程中其抗原性又有一定程度的升高。 展开更多
关键词 德氏乳杆菌 发酵样品 保加利亚 抗原性 酪蛋白 牛乳 亚种 ELISA法
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Promotion of Safe Winemaking Practices Using Quantity Comparison and Methanol-reduction Process for Rice Wine and Whisky
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作者 Wachira Singkong Boonyakrit Rattanapun Kritsada Kaweewong 《Journal of Agricultural Science and Technology(B)》 2012年第4期448-454,共7页
Research was conducted to determine the quantity of methanol in liquor locally produced in nine different areas of Kamphaengphet province, Thailand. All samples were investigated for methanol content and, while quanti... Research was conducted to determine the quantity of methanol in liquor locally produced in nine different areas of Kamphaengphet province, Thailand. All samples were investigated for methanol content and, while quantities varied depending on the fermentation process, the amounts of methanol determined were statistically significant. The results showed a clear trend of increasing amounts of methanol; the longer it was fermented, the higher amount of methanol it gained. The appropriate distillation temperature was at 85 ℃ examined from a fermented sample which had the highest level of methanol. As a part of this research, a workshop was conducted for distillers on skill development and knowledge promotion for rice wine, rice whisky and safe wine manufacturer. This included a campaign to promote safer rice wine distillation process wherein alcohol content does not exceed 15%. An evaluation at the completion of the workshop indicated that the participants had gained knowledge and understanding, satisfaction, and knowledge utilization. 展开更多
关键词 ALCOHOL DISTILLATION starter culture knowledge promotion Thailand training.
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Safety and Quality Attributes of Sucuk-Like Product Made with Mechanically Deboned Broiler/Beef
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作者 Songul Sahin Ercan Huseyin Bozkurt Cigdem Soysal 《Journal of Food Science and Engineering》 2013年第5期246-254,共9页
In this study, sucuk-like products (SLP) has a great consumption in Turkey, which are produced with broiler or mixture of beef and broiler meat and after stuffing into artificial casings heat treatment is applied af... In this study, sucuk-like products (SLP) has a great consumption in Turkey, which are produced with broiler or mixture of beef and broiler meat and after stuffing into artificial casings heat treatment is applied after rapid fermentation. Quality (color, pH, texture) and safety (2-thiobarbituric acid reactive substance (TBARS) value and biogenic amine) attributes of SLP made with broiler, mechanically deboned meat (MDM) of broiler chicken, mix of broiler chicken and beef, or mix of MDM of broiler chicken and MDM of beef were investigated, pH values of samples had similar range. Mechanically deboned meat containing samples have higher TBARS values than that of others. Samples made with MDM of broiler chicken have higher histamine, tyramine, putrescine and total biogenic amine concentrations than samples made with broiler chicken. There was no significant difference (P 〉 0.05) between total biogenic amine contents of fermented and non-fermented samples. Also, samples gave similar measured and calculated color values. Hardness, gumminess and chewiness values of samples made with MDM of broiler chicken were significantly higher (P 〈 0.05) than samples made with broiler chicken. It was observed that addition of mechanically deboned meat could result in loss of safety and quality attributes of sucuk-like products. 展开更多
关键词 Sucuk sucuk-like product MDM biogenic amines TEXTURE
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