The surface ultrastructure of mature egg chorion of the tamed northern sheatfish Silurus soldatovi in Heilongjiang river was investigated by scanning electron microscopy. The outermost layer was composed of a thin lam...The surface ultrastructure of mature egg chorion of the tamed northern sheatfish Silurus soldatovi in Heilongjiang river was investigated by scanning electron microscopy. The outermost layer was composed of a thin lamella of a jelly coat. The surface of jelly coat was characterized by overlapping net-like fibrillar lamellae, many clefts with bridges and pores. The pore canals were 0.25 μm in diameter. The pore density was about 0.7-1.0/μm-2. The pores on the surface of jelly coart were distributed uniformly. Mature eggs of sheatfish possessed a funnel-shaped micropyle in the animal polar region. The micropylar region was about 0.04 mm2 area in diameter with 3-8 ridges in radial arrangement. Fertilized eggs in which jelly coats were removed mechanically presented no adhesiveness and exhibited inlays of irregular granules on their surface.展开更多
Using conventional histochemical methods and scanning electron microscope,the egg surface of mature oocyte of Pelteobagrus vachelli was observed. The micropyle is funnel-like and consists of micropylar vestibule and m...Using conventional histochemical methods and scanning electron microscope,the egg surface of mature oocyte of Pelteobagrus vachelli was observed. The micropyle is funnel-like and consists of micropylar vestibule and micropylar canal. The opening of micropylar vestibule is about 11.35 μm in diameter while entrance of the micropyle to the oocyte is only about 2.6 μm. There are plenty of micropores on the surface near the micropyle. Micropyle might act as an apparatus leading spermatozoa to penetrating an oocyte. Regular cord-like structures between basal membrane (the outermost layer of egg surface) and plasmic membrane (the inner layer of egg surface) are found. These cord-like structures, each about 16.75 to 18.50 μm in length, 5 μm in diameter, comprised small balls whose diameters vary from 2.18 to 2.5 μm and are arranged compactly under the basal membrane. These kinds of structures are zona radiata and act as special eggshell. After activation of the oocytes, the cord-like structures would partially transform into mucoprotein. To our best knowledge, this paper is the first report on stereo view of the zona radiata of teleost oocyte.展开更多
香鱼（Plecoglossus altivelis Temminck et Schlegel）俗称留香鱼、海胎鱼，日本人称为鲇鱼，是我国一种小型名贵经济鱼类，其肉质细嫩，味道鲜美，已在浙江、福建、广东、山东等地陆续开展了人工养殖。香鱼系裸卵巢，适当成熟卵母细...香鱼（Plecoglossus altivelis Temminck et Schlegel）俗称留香鱼、海胎鱼，日本人称为鲇鱼，是我国一种小型名贵经济鱼类，其肉质细嫩，味道鲜美，已在浙江、福建、广东、山东等地陆续开展了人工养殖。香鱼系裸卵巢，适当成熟卵母细胞排入到腹腔中，经历96h后仍能受精孵化，其受精率与孵化率分别还有10．83％和0．53％。展开更多
文摘The surface ultrastructure of mature egg chorion of the tamed northern sheatfish Silurus soldatovi in Heilongjiang river was investigated by scanning electron microscopy. The outermost layer was composed of a thin lamella of a jelly coat. The surface of jelly coat was characterized by overlapping net-like fibrillar lamellae, many clefts with bridges and pores. The pore canals were 0.25 μm in diameter. The pore density was about 0.7-1.0/μm-2. The pores on the surface of jelly coart were distributed uniformly. Mature eggs of sheatfish possessed a funnel-shaped micropyle in the animal polar region. The micropylar region was about 0.04 mm2 area in diameter with 3-8 ridges in radial arrangement. Fertilized eggs in which jelly coats were removed mechanically presented no adhesiveness and exhibited inlays of irregular granules on their surface.
文摘Using conventional histochemical methods and scanning electron microscope,the egg surface of mature oocyte of Pelteobagrus vachelli was observed. The micropyle is funnel-like and consists of micropylar vestibule and micropylar canal. The opening of micropylar vestibule is about 11.35 μm in diameter while entrance of the micropyle to the oocyte is only about 2.6 μm. There are plenty of micropores on the surface near the micropyle. Micropyle might act as an apparatus leading spermatozoa to penetrating an oocyte. Regular cord-like structures between basal membrane (the outermost layer of egg surface) and plasmic membrane (the inner layer of egg surface) are found. These cord-like structures, each about 16.75 to 18.50 μm in length, 5 μm in diameter, comprised small balls whose diameters vary from 2.18 to 2.5 μm and are arranged compactly under the basal membrane. These kinds of structures are zona radiata and act as special eggshell. After activation of the oocytes, the cord-like structures would partially transform into mucoprotein. To our best knowledge, this paper is the first report on stereo view of the zona radiata of teleost oocyte.
文摘香鱼（Plecoglossus altivelis Temminck et Schlegel）俗称留香鱼、海胎鱼，日本人称为鲇鱼，是我国一种小型名贵经济鱼类，其肉质细嫩，味道鲜美，已在浙江、福建、广东、山东等地陆续开展了人工养殖。香鱼系裸卵巢，适当成熟卵母细胞排入到腹腔中，经历96h后仍能受精孵化，其受精率与孵化率分别还有10．83％和0．53％。
