AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METH...AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METHODS: Eighty paraffin-embedded cancer tissue samples were selected from primary colorectal adenocarcinoma. Twenty-eight patients had well-differentiated, 22 had moderately differentiated and 30 had poorly differentiated adenocarcinoma. Forty-five patients and 35 patients had lymph node metastasis. Forty-five patients were of Dukes A to B stage, and 35 were of C to D stage. Another 22 paraffi n-embedded tissue blocks of normal colorectal epithelium (>5 cm away from the edge of the tumor) were selected as the control group. All patients with colorectal cancer were treated surgically and diagnosed histologically, without preoperative chemotherapy or radiotherapy. The immunohistochemistry was used to detect the ezrin expression in paraffin-embedded normal colorectal mucosa tissues and colorectal cancer tissue samples.RESULTS: Ezrin expression in colorectal cancer was significantly higher than in normal colorectal mucosa (75.00% vs 9.09%, P<0.01), and there was a close relationship between ezrin expression and the degree of tumor differentiation, lymph node metastasis and Dukes stage (88.46% vs 50.00%, P<0.01; 94.28% vs 51.11%, P<0.01; 94.28% vs 51.11%, P<0.01).CONCLUSION: Ezrin expression is obviously higher in colorectal cancer tissues than in normal colorectal mucosa tissues, and the high level of ezrin expression is closely related to the colorectal cancer invasion and metastasis process.展开更多
荧光原位杂交（fluorescence in situ hybridisation，FISH）是在石蜡包埋组织中检测细胞染色体变化及基因异常的一种敏感性强、特异性高的分子生物学方法。对石蜡包埋组织进行FISH染色所采用的方法有两种，在提取的细胞核上进行和直接...荧光原位杂交（fluorescence in situ hybridisation，FISH）是在石蜡包埋组织中检测细胞染色体变化及基因异常的一种敏感性强、特异性高的分子生物学方法。对石蜡包埋组织进行FISH染色所采用的方法有两种，在提取的细胞核上进行和直接在石蜡包埋组织切片上进行。然而，从石蜡包埋组织中提取细胞核进行FISH的方法复杂、耗时，而且所需组织较多，展开更多
目的探讨实时荧光定量PCR(real time PCR,RT-PCR)与抗酸染色法检测石蜡包埋骨关节组织中结核分枝杆菌在辅助诊断结核病的作用。方法回顾性分析253例骨关节石蜡包埋组织RT-PCR与抗酸染色法检测结核杆菌结果,并与临床结果对比分析检测的...目的探讨实时荧光定量PCR(real time PCR,RT-PCR)与抗酸染色法检测石蜡包埋骨关节组织中结核分枝杆菌在辅助诊断结核病的作用。方法回顾性分析253例骨关节石蜡包埋组织RT-PCR与抗酸染色法检测结核杆菌结果,并与临床结果对比分析检测的灵敏度及特异性。结果253例组织形态学表现为肉芽肿性炎伴或不伴坏死的送检样本中,RT-PCR检出率为38.3%(97/253),抗酸染色检出率为41.5%(105/253),两种检测的符合率为88.1%。RT-PCR检测的灵敏度、特异性分别为89.9%、94.8%。抗酸染色检测的灵敏度、特异性分别为85.9%、87.0%。两种检测均阳性比仅抗酸染色阳性与临床诊断结果的符合率差异有统计学意义(P<0.05);仅RT-PCR阳性比仅抗酸染色阳性与临床诊断结果的符合率差异有统计学意义(P<0.05)。结论同时行RT-PCR与抗酸染色检测有助于石蜡包埋骨关节组织中快速、准确诊断结核病。可用于疑似骨关节结核的肉芽肿性病变的鉴别诊断,具有较高的临床应用价值。展开更多
基金Supported by Natural Science Foundation of Shanghai,No.04ZB14072
文摘AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METHODS: Eighty paraffin-embedded cancer tissue samples were selected from primary colorectal adenocarcinoma. Twenty-eight patients had well-differentiated, 22 had moderately differentiated and 30 had poorly differentiated adenocarcinoma. Forty-five patients and 35 patients had lymph node metastasis. Forty-five patients were of Dukes A to B stage, and 35 were of C to D stage. Another 22 paraffi n-embedded tissue blocks of normal colorectal epithelium (>5 cm away from the edge of the tumor) were selected as the control group. All patients with colorectal cancer were treated surgically and diagnosed histologically, without preoperative chemotherapy or radiotherapy. The immunohistochemistry was used to detect the ezrin expression in paraffin-embedded normal colorectal mucosa tissues and colorectal cancer tissue samples.RESULTS: Ezrin expression in colorectal cancer was significantly higher than in normal colorectal mucosa (75.00% vs 9.09%, P<0.01), and there was a close relationship between ezrin expression and the degree of tumor differentiation, lymph node metastasis and Dukes stage (88.46% vs 50.00%, P<0.01; 94.28% vs 51.11%, P<0.01; 94.28% vs 51.11%, P<0.01).CONCLUSION: Ezrin expression is obviously higher in colorectal cancer tissues than in normal colorectal mucosa tissues, and the high level of ezrin expression is closely related to the colorectal cancer invasion and metastasis process.
文摘荧光原位杂交（fluorescence in situ hybridisation，FISH）是在石蜡包埋组织中检测细胞染色体变化及基因异常的一种敏感性强、特异性高的分子生物学方法。对石蜡包埋组织进行FISH染色所采用的方法有两种，在提取的细胞核上进行和直接在石蜡包埋组织切片上进行。然而，从石蜡包埋组织中提取细胞核进行FISH的方法复杂、耗时，而且所需组织较多，
文摘目的探讨实时荧光定量PCR(real time PCR,RT-PCR)与抗酸染色法检测石蜡包埋骨关节组织中结核分枝杆菌在辅助诊断结核病的作用。方法回顾性分析253例骨关节石蜡包埋组织RT-PCR与抗酸染色法检测结核杆菌结果,并与临床结果对比分析检测的灵敏度及特异性。结果253例组织形态学表现为肉芽肿性炎伴或不伴坏死的送检样本中,RT-PCR检出率为38.3%(97/253),抗酸染色检出率为41.5%(105/253),两种检测的符合率为88.1%。RT-PCR检测的灵敏度、特异性分别为89.9%、94.8%。抗酸染色检测的灵敏度、特异性分别为85.9%、87.0%。两种检测均阳性比仅抗酸染色阳性与临床诊断结果的符合率差异有统计学意义(P<0.05);仅RT-PCR阳性比仅抗酸染色阳性与临床诊断结果的符合率差异有统计学意义(P<0.05)。结论同时行RT-PCR与抗酸染色检测有助于石蜡包埋骨关节组织中快速、准确诊断结核病。可用于疑似骨关节结核的肉芽肿性病变的鉴别诊断,具有较高的临床应用价值。
