To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7...To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM R T Easy Vectors After that, the specific fragments were sequenced by an automated DNA sequencer Results Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length All 5 point mutations were located in two unique sequence blocks (UQ Ⅰ and UQ Ⅱ), and no insertions or deletions were found The identities of comparison of Leishmania in GeneBank were more than 98% Conclusion Five point mutations exist in the SSU rDNA variable region of 5 L d isolates from different epidemic foci of visceral leishmaniasis (VL) in China Sequence differences of the SSU rDNA variable region exist among L d isolates from different foci展开更多
目的分析2005—2015年新疆克孜勒苏柯尔克孜自治州（简称克州）内脏利什曼病的流行趋势,为制定和采取相应的防治对策提供依据。方法汇总2005—2015年克州内脏利什曼病的报告数据,采用Microsoft Excel 2010和Epi Info 3.5.3对资料进行分...目的分析2005—2015年新疆克孜勒苏柯尔克孜自治州（简称克州）内脏利什曼病的流行趋势,为制定和采取相应的防治对策提供依据。方法汇总2005—2015年克州内脏利什曼病的报告数据,采用Microsoft Excel 2010和Epi Info 3.5.3对资料进行分析。结果 11年间克州以2005年病例报告最多（21例）、占27.27%（21/77）,2015年最少、无病例,差异有统计学意义（χ2=21.21,P〈0.05）;发病高峰期病例占全年的58.45%（45/77）,发病低峰期占全年的41.55%（32/77）,差异有统计学意义（χ2=4.39,P〈0.05）;1岁-组病例数最多、占29.87%（23/77）,21岁-组病例数最少、占2.60%（2/77）,差异有统计学意义（χ2=15.93,P〈0.05）;阿图什市阿扎克乡病例数最多（29例）、占37.66%（29/77）,阿克陶县奥依塔克镇和克孜勒陶乡病例数最少（各1例）、各占1.30%（1/77）,差异有统计学意义（χ2=32.46,P〈0.05）。结论如果不能有效地控制传染源和传播媒介,克州将会出现更多的内脏利什曼病新疫区和新发患者。展开更多
基金ThisprojectwassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 39970 667)
文摘To confirm the existence of point mutations in the SSU rDNA variable regions of 5 Leishmania donovani (L d ) isolates from different epidemic foci in China Methods Specific SSU rDNA fragments from nuclear DNA of 7 Leishmania species/isolates were amplified by PCR and then cloned into pGEM R T Easy Vectors After that, the specific fragments were sequenced by an automated DNA sequencer Results Sequence analysis showed that the amplified DNA fragments of 7 Leishmania species/isolates were all 392 bp in length All 5 point mutations were located in two unique sequence blocks (UQ Ⅰ and UQ Ⅱ), and no insertions or deletions were found The identities of comparison of Leishmania in GeneBank were more than 98% Conclusion Five point mutations exist in the SSU rDNA variable region of 5 L d isolates from different epidemic foci of visceral leishmaniasis (VL) in China Sequence differences of the SSU rDNA variable region exist among L d isolates from different foci
