目的研究不同海拔高度的低氧环境差异对内皮PAS蛋白1基因（endothelial PAS domain protein1，EPAS1）的选择作用。方法选取西藏藏族58名（居住海拔约3700米）、青海藏族47名（居住海拔约3100米）、云南藏族43名（居住海拔约2500米）、...目的研究不同海拔高度的低氧环境差异对内皮PAS蛋白1基因（endothelial PAS domain protein1，EPAS1）的选择作用。方法选取西藏藏族58名（居住海拔约3700米）、青海藏族47名（居住海拔约3100米）、云南藏族43名（居住海拔约2500米）、山东汉族47名（居住海拔约50米）的4个不同海拔高度的群体，应用限制性片段长度多态性一聚合酶链反应技术检测EPAS1基因的14个单核苷酸多态性位点。结果在4个群体中，山东汉族、云南藏族与西藏藏族、青海藏族在大部分位点的等位基因频率、基因型频率以及单倍型频率的差异具有统计学意义；但在山东汉族与云南藏族之间的差异无统计学意义。结论海拔高度不同引起的低氧环境差异有可能对基因EPASj有选择作用。展开更多
AIM:To investigate the expression of hypoxia-inducible factor (HIF)-2α/endothelial PAS domain protein1 (EPAS1) in hepatocellular carcinoma (HCC).METHODS: Expression of HIF-2α/EPAS1 was investigated immunohistochemic...AIM:To investigate the expression of hypoxia-inducible factor (HIF)-2α/endothelial PAS domain protein1 (EPAS1) in hepatocellular carcinoma (HCC).METHODS: Expression of HIF-2α/EPAS1 was investigated immunohistochemically on paraffin-embedded sections from 97 patients with HCC.To further confirm that HIF-2α/EPAS1 in HCC tissues also correlated with angiogenesis, a parallel immunohistchemistry study of vascular endothelial growth factor (VEGF) was performed on these 97 cases.RESULTS:HIF-2α/EPAS1 could be detected in 50 of 97 cases (51.6%), including 19 weakly positive (19.8%), and 31 strongly positive (31.1%), the other 47 cases were negative(48.4%). The expression of HIF-2α/EPASlwas significantly correlated with tumor size,capsule infiltration, portal vein invasion, and necrosis. A parallel immunohistochemical analysis of VEGF demonstrated its positive correlation with capsule infiltration, portal vein invasion, and HIF-α/EPAS1 overexpression, which supported the correlation of HIF-2α/EPASlup-regulation with tumor angiogenesis. No apparent correlation was observed between HIF-2α/EPAS1 and capsular formation, presence of cirrhosis, and histological grade.CONCLUSION: HIF-2α/EPAS1 is expressed in most of HCC with capsular infiltration and portal vein invasion, which indicates a possible role of HIF-2α/EPAS1 in HCC metastasis.展开更多
文摘目的研究不同海拔高度的低氧环境差异对内皮PAS蛋白1基因（endothelial PAS domain protein1，EPAS1）的选择作用。方法选取西藏藏族58名（居住海拔约3700米）、青海藏族47名（居住海拔约3100米）、云南藏族43名（居住海拔约2500米）、山东汉族47名（居住海拔约50米）的4个不同海拔高度的群体，应用限制性片段长度多态性一聚合酶链反应技术检测EPAS1基因的14个单核苷酸多态性位点。结果在4个群体中，山东汉族、云南藏族与西藏藏族、青海藏族在大部分位点的等位基因频率、基因型频率以及单倍型频率的差异具有统计学意义；但在山东汉族与云南藏族之间的差异无统计学意义。结论海拔高度不同引起的低氧环境差异有可能对基因EPASj有选择作用。
基金Supported by the National Key Technologies R and D Program,No.2001BA703B04
文摘AIM:To investigate the expression of hypoxia-inducible factor (HIF)-2α/endothelial PAS domain protein1 (EPAS1) in hepatocellular carcinoma (HCC).METHODS: Expression of HIF-2α/EPAS1 was investigated immunohistochemically on paraffin-embedded sections from 97 patients with HCC.To further confirm that HIF-2α/EPAS1 in HCC tissues also correlated with angiogenesis, a parallel immunohistchemistry study of vascular endothelial growth factor (VEGF) was performed on these 97 cases.RESULTS:HIF-2α/EPAS1 could be detected in 50 of 97 cases (51.6%), including 19 weakly positive (19.8%), and 31 strongly positive (31.1%), the other 47 cases were negative(48.4%). The expression of HIF-2α/EPASlwas significantly correlated with tumor size,capsule infiltration, portal vein invasion, and necrosis. A parallel immunohistochemical analysis of VEGF demonstrated its positive correlation with capsule infiltration, portal vein invasion, and HIF-α/EPAS1 overexpression, which supported the correlation of HIF-2α/EPASlup-regulation with tumor angiogenesis. No apparent correlation was observed between HIF-2α/EPAS1 and capsular formation, presence of cirrhosis, and histological grade.CONCLUSION: HIF-2α/EPAS1 is expressed in most of HCC with capsular infiltration and portal vein invasion, which indicates a possible role of HIF-2α/EPAS1 in HCC metastasis.
