PURPOSE: To examine the association of systemic markers of inflammatory disease and endothelial dysfunction with age. related maculopathy (ARM). DESIGN: (1) Nested case-control analysis of prevalent ARM and (2) prospe...PURPOSE: To examine the association of systemic markers of inflammatory disease and endothelial dysfunction with age. related maculopathy (ARM). DESIGN: (1) Nested case-control analysis of prevalent ARM and (2) prospective analyses of incident ARM in a random sample of a population-based cohort. METHODS: Standardized protocols for blood collection, measurement of markers, administration of a questionnaire, and gradings of stereoscopic color fundus photography to determine ARM were used. Standard univariate and multivariate analyses were performed. participants: Included in the nested case-control study were 188 cases with moderate to advanced ARM and 195 controls matched for age, gender, and current smoking status at a baseline examination from 1988 to 1990, and living in Beaver Dam, Wisconsin. Included in the prospective analyses as a random sample of 321 persons were those who participated in a 5- year and/or 10- year follow-up. main outcome measures: Prevalent and incident ARM. RESULTS: Serum C-reactive protein, amyloid A, interleukin- 6, tumor necrosis factor-α , intracellular adhesion molecule, Eselectin, folate, and Chlamydia pneumoniae IgG antibody were not associated with either prevalent or incident ARM. CONCLUSION: Contrary to other reports, we cannot confirm a strong or consistent relationship of markers of inflammation and endothelial dysfunction with ARM.展开更多
Background: Risk factors for atherosclerosis such as hypertension, type 2 diabetes, obesity and dyslipidemia affect endothelial function and stimulate adhesion molecules expression. The aim of the study was to examine...Background: Risk factors for atherosclerosis such as hypertension, type 2 diabetes, obesity and dyslipidemia affect endothelial function and stimulate adhesion molecules expression. The aim of the study was to examine endothelial activation in type 2 diabetes and hypertension as indicated by adhesion molecule levels and further to investigate whether the coexistence of the above conditions has a different effect. Methods: Serum levels of soluble E-selectin, ICAM-1 and VCAM-1 were measured in 17 hypertensive type 2 diabetic patients(DM-HY), 32 normotensive type 2 diabetic patients(DM), 11 hypertensive nondiabetic patients(HY) and 15 healthy subjects. Results: In diabetic patients(either DM-HY or DM), soluble E-selectin levels were significantly increased compared to healthy subjects(p< 0.001). In HY patients, both sE-selectin(66.44±71.59 vs. 29.42±15.56 ng/ml, p=0.033) and sVCAM-1(1529±433.33 vs. 1027±243.56 ng/ml, p=0.03) levels were found significantly higher compared to healthy subjects(p< 0.05). The coexistence of diabetes and hypertension(DM-HY) did not have an additive effect on circulating adhesion molecules levels compared with the levels observed in either diabetes or hypertension. Systolic and diastolic blood pressure(BP) were independent factors correlated respectively with sE-selectin and sVCAM-1 levels(R=0.454, p=0.034 and R=0.578, p=0.005) in nondiabetic subjects(hypertensive and normotensive). Conclusions: Type 2 diabetes mellitus and hypertension induce endothelial activation as indicated by elevated levels of soluble adhesion molecules. This effect is not different when comorbidity is present.展开更多
Traditionally the evaluation of the cellular infiltrate and protein expression in skin tissue sections is done by manual quantification. However, for reliable evaluation of histology in the development of new anti-pso...Traditionally the evaluation of the cellular infiltrate and protein expression in skin tissue sections is done by manual quantification. However, for reliable evaluation of histology in the development of new anti-psoriatic treatments there is a need for a more time-efficient and reproducible method. To test the use of digital image analysis (DIA) in this situation we compared the assessment of immunohistochemically stained skin sections with themore traditionalmanual quantification (MQ) and semiquantitative analysis (SQA). The number of CD3+T cells and the expression of E-selectin were evaluated in stained paired skin biopsie s from 11 patients with chronic plaque psoriasis before and after initiation of anti-psoriasis therapy. We observed significant correlations between MQ and DIA for the number of T cells (epiderm is: r=0.88, P ≤0.01, dermis r=0.87, P ≤0.01). Both DIA and MQ were equally eff ective in detecting reductions of T-cell numbers in active-treated patients. M Q took 20 h, compared to 6 h for DIA.We also observed significant correlations b etween SQA and DIA for the expression of E-selectin (r=0.88, P ≤0.01), althoug h DIA was more sensitive than SQA to detect (early) changes. SQA took 10 h, comp ared to 4 h for DIA. In conclusion, the quantification of the inflammatory infil trate in psoriatic lesional skin by DIA generated similar results as MQ and SQA in a reliable, reproducible and higher time efficient fashion.展开更多
文摘PURPOSE: To examine the association of systemic markers of inflammatory disease and endothelial dysfunction with age. related maculopathy (ARM). DESIGN: (1) Nested case-control analysis of prevalent ARM and (2) prospective analyses of incident ARM in a random sample of a population-based cohort. METHODS: Standardized protocols for blood collection, measurement of markers, administration of a questionnaire, and gradings of stereoscopic color fundus photography to determine ARM were used. Standard univariate and multivariate analyses were performed. participants: Included in the nested case-control study were 188 cases with moderate to advanced ARM and 195 controls matched for age, gender, and current smoking status at a baseline examination from 1988 to 1990, and living in Beaver Dam, Wisconsin. Included in the prospective analyses as a random sample of 321 persons were those who participated in a 5- year and/or 10- year follow-up. main outcome measures: Prevalent and incident ARM. RESULTS: Serum C-reactive protein, amyloid A, interleukin- 6, tumor necrosis factor-α , intracellular adhesion molecule, Eselectin, folate, and Chlamydia pneumoniae IgG antibody were not associated with either prevalent or incident ARM. CONCLUSION: Contrary to other reports, we cannot confirm a strong or consistent relationship of markers of inflammation and endothelial dysfunction with ARM.
文摘Background: Risk factors for atherosclerosis such as hypertension, type 2 diabetes, obesity and dyslipidemia affect endothelial function and stimulate adhesion molecules expression. The aim of the study was to examine endothelial activation in type 2 diabetes and hypertension as indicated by adhesion molecule levels and further to investigate whether the coexistence of the above conditions has a different effect. Methods: Serum levels of soluble E-selectin, ICAM-1 and VCAM-1 were measured in 17 hypertensive type 2 diabetic patients(DM-HY), 32 normotensive type 2 diabetic patients(DM), 11 hypertensive nondiabetic patients(HY) and 15 healthy subjects. Results: In diabetic patients(either DM-HY or DM), soluble E-selectin levels were significantly increased compared to healthy subjects(p< 0.001). In HY patients, both sE-selectin(66.44±71.59 vs. 29.42±15.56 ng/ml, p=0.033) and sVCAM-1(1529±433.33 vs. 1027±243.56 ng/ml, p=0.03) levels were found significantly higher compared to healthy subjects(p< 0.05). The coexistence of diabetes and hypertension(DM-HY) did not have an additive effect on circulating adhesion molecules levels compared with the levels observed in either diabetes or hypertension. Systolic and diastolic blood pressure(BP) were independent factors correlated respectively with sE-selectin and sVCAM-1 levels(R=0.454, p=0.034 and R=0.578, p=0.005) in nondiabetic subjects(hypertensive and normotensive). Conclusions: Type 2 diabetes mellitus and hypertension induce endothelial activation as indicated by elevated levels of soluble adhesion molecules. This effect is not different when comorbidity is present.
文摘Traditionally the evaluation of the cellular infiltrate and protein expression in skin tissue sections is done by manual quantification. However, for reliable evaluation of histology in the development of new anti-psoriatic treatments there is a need for a more time-efficient and reproducible method. To test the use of digital image analysis (DIA) in this situation we compared the assessment of immunohistochemically stained skin sections with themore traditionalmanual quantification (MQ) and semiquantitative analysis (SQA). The number of CD3+T cells and the expression of E-selectin were evaluated in stained paired skin biopsie s from 11 patients with chronic plaque psoriasis before and after initiation of anti-psoriasis therapy. We observed significant correlations between MQ and DIA for the number of T cells (epiderm is: r=0.88, P ≤0.01, dermis r=0.87, P ≤0.01). Both DIA and MQ were equally eff ective in detecting reductions of T-cell numbers in active-treated patients. M Q took 20 h, compared to 6 h for DIA.We also observed significant correlations b etween SQA and DIA for the expression of E-selectin (r=0.88, P ≤0.01), althoug h DIA was more sensitive than SQA to detect (early) changes. SQA took 10 h, comp ared to 4 h for DIA. In conclusion, the quantification of the inflammatory infil trate in psoriatic lesional skin by DIA generated similar results as MQ and SQA in a reliable, reproducible and higher time efficient fashion.
