Mononuclear cells （MNCs） isolated from peripheral blood by density gradient centrifugation were plated on human fibronectin-coated culture plates and cultured in EGM-2 medium. Attached spindle-shaped cells, reported...Mononuclear cells （MNCs） isolated from peripheral blood by density gradient centrifugation were plated on human fibronectin-coated culture plates and cultured in EGM-2 medium. Attached spindle-shaped cells, reported as endothelial progenitor cells （EPCs） by some investigators, had elongated from adherent round cells, but had not proliferated from a small number of cells as supposed previously. The growth curve of the primary EPCs showed that the cells had little proliferative capacity. Flow cytometry analysis showed that the cells could express some of the endothelial lineage markers, while they could also express CD 14, which is considered a marker of monocyte/macrophage lineages throughout culture. In endothelial function assays, the cells demonstrated a lower level of expression of eNOS than mature endothelial cells in the reverse transcription-polymerase chain reaction and did not show an ability to develop tube-like structures in angiogenesis assay in vitro. In this study, we identified the monocytoid function of EPCs by the combined Dillabeled acetylated low-density lipoprotein （Dil-Ac-LDL） and Indian ink uptake tests. All the cells were double positive for Dil- Ac-LDL and Indian ink uptake at days 4, 14 and 28 of culture, which means the EPCs maintained monocytoid function throughout the culture. Therefore, although adult EPCs from peripheral MNCs have some endothelial lineage properties, they maintain typical monocytic function and have little proliferative capacity.展开更多
近日,中国科学院分子细胞科学卓越创新中心(生物化学与细胞生物学研究所)曾艺研究团队以"Activation of Wnt/β-catenin signaling by Zeb1 in endothelial progenitors induces vascular quiescence entry"为题在Cell Report...近日,中国科学院分子细胞科学卓越创新中心(生物化学与细胞生物学研究所)曾艺研究团队以"Activation of Wnt/β-catenin signaling by Zeb1 in endothelial progenitors induces vascular quiescence entry"为题在Cell Reports上在线发表最新研究成果.该研究揭示了Zeb1通过调节Wnt/β-catenin信号通路调控血管内皮干细胞静息状态的机制.展开更多
Adult stem/progenitor cells play important roles in tissue homeostasis and have important implications for regenerativemedicine.It was once thought that formation of new blood vessels in adult only occurs through angi...Adult stem/progenitor cells play important roles in tissue homeostasis and have important implications for regenerativemedicine.It was once thought that formation of new blood vessels in adult only occurs through angiogenesis,a processwhereby new vessels are formed from existing mature endothelial cells;while vasculogenesis,where new vessels arederived from differentiation of endothelial progenitor cells(EPCs),was thought to occur exclusively in embryos.Thediscovery of adult EPCs a few years ago has changed this old paradigm;and subsequent studies showed that EPCs maybe a promising tool for the treatment of vascular disorders.However,there have been conflicting reports on subtypes,surface markers,and functions of EPCs;and thus the exact origin and identity of EPCs remain to be defined.A commonapproach to obtain EPCs is to isolate and culture mononuclear cells from peripheral blood and to select adherent cells展开更多
基金the National Natural Science Foundation of China （Nos. 30170932 , 30371411） the Foundation for Excellent Young Scholar （No. 30125039）.
文摘Mononuclear cells （MNCs） isolated from peripheral blood by density gradient centrifugation were plated on human fibronectin-coated culture plates and cultured in EGM-2 medium. Attached spindle-shaped cells, reported as endothelial progenitor cells （EPCs） by some investigators, had elongated from adherent round cells, but had not proliferated from a small number of cells as supposed previously. The growth curve of the primary EPCs showed that the cells had little proliferative capacity. Flow cytometry analysis showed that the cells could express some of the endothelial lineage markers, while they could also express CD 14, which is considered a marker of monocyte/macrophage lineages throughout culture. In endothelial function assays, the cells demonstrated a lower level of expression of eNOS than mature endothelial cells in the reverse transcription-polymerase chain reaction and did not show an ability to develop tube-like structures in angiogenesis assay in vitro. In this study, we identified the monocytoid function of EPCs by the combined Dillabeled acetylated low-density lipoprotein （Dil-Ac-LDL） and Indian ink uptake tests. All the cells were double positive for Dil- Ac-LDL and Indian ink uptake at days 4, 14 and 28 of culture, which means the EPCs maintained monocytoid function throughout the culture. Therefore, although adult EPCs from peripheral MNCs have some endothelial lineage properties, they maintain typical monocytic function and have little proliferative capacity.
文摘近日,中国科学院分子细胞科学卓越创新中心(生物化学与细胞生物学研究所)曾艺研究团队以"Activation of Wnt/β-catenin signaling by Zeb1 in endothelial progenitors induces vascular quiescence entry"为题在Cell Reports上在线发表最新研究成果.该研究揭示了Zeb1通过调节Wnt/β-catenin信号通路调控血管内皮干细胞静息状态的机制.
文摘Adult stem/progenitor cells play important roles in tissue homeostasis and have important implications for regenerativemedicine.It was once thought that formation of new blood vessels in adult only occurs through angiogenesis,a processwhereby new vessels are formed from existing mature endothelial cells;while vasculogenesis,where new vessels arederived from differentiation of endothelial progenitor cells(EPCs),was thought to occur exclusively in embryos.Thediscovery of adult EPCs a few years ago has changed this old paradigm;and subsequent studies showed that EPCs maybe a promising tool for the treatment of vascular disorders.However,there have been conflicting reports on subtypes,surface markers,and functions of EPCs;and thus the exact origin and identity of EPCs remain to be defined.A commonapproach to obtain EPCs is to isolate and culture mononuclear cells from peripheral blood and to select adherent cells
