目的:通过观察低频电针对海洛因心理依赖小鼠海马N-甲基-D-天冬氨酸(NMDA)的亚型NMDAR1(NR1)、NM-DAR-2A(NR2A)、NMDAR-2B(NR2B)表达的影响,探讨低频电针对海洛因成瘾记忆干预作用的可能中枢机制。方法:筛选无天然位置偏爱的雄性小鼠45...目的:通过观察低频电针对海洛因心理依赖小鼠海马N-甲基-D-天冬氨酸(NMDA)的亚型NMDAR1(NR1)、NM-DAR-2A(NR2A)、NMDAR-2B(NR2B)表达的影响,探讨低频电针对海洛因成瘾记忆干预作用的可能中枢机制。方法:筛选无天然位置偏爱的雄性小鼠45只,随机分为对照组、海洛因心理依赖模型组(模型组)、模型电针组(模针组),每组15只。海洛因剂量递增注射结合条件性位置偏爱(conditioned place preference,CPP)训练制作海洛因心理依赖模型,2Hz低频电针"内关"、"三阴交",点刺"四神聪"穴。采用白箱CPP值变化评价低频电针治疗效应,免疫组化方法检测海马NMDA受体亚型(NR1、NR2A、NR2B)的表达。结果:①模针组白箱CPP值较模型组明显降低(P<0.05)。②模针组小鼠NR1、NR2B阳性表达较模型组增强(P<0.05)。③模针组小鼠NR2A阳性表达较模型组降低(P<0.05)。结论:低频电针疗法能增强海洛因心理依赖小鼠海马NR1、NR2B,降低NR2A的阳性表达。这种改变可能与低频电针调节海洛因依赖的机制有关。展开更多
Objective To explore the difference of low-frequency electroacupuncture in regulating serum leptin and neuropeptide Y（NPY） of male and female patients with simple obesity due to spleen deficiency and damp exuberance...Objective To explore the difference of low-frequency electroacupuncture in regulating serum leptin and neuropeptide Y（NPY） of male and female patients with simple obesity due to spleen deficiency and damp exuberance. Methods Eighty patients with simple obesity, including 37 males and 43 females, were included in this study. In both groups（male group and female group）, Yīnlíngquán（阴陵泉 SP 9）, Sānyīnjiāo（三阴交 SP 6）, Zúsānlǐ（足三里 ST 36）, Fēnglóng（丰隆 ST 40）, Qūchí（曲池 LI 11）, Tiānshū（天枢 ST 25）, Zhōngwǎn（中脘 CV 12）, Shuǐfēn（水分 CV 9）, Qìhǎi（气海 CV 6）, and Guānyuán（关元 CV 4）. were selected, and low-frequency electroacupuncture treatment was applied. Needles were retained for 30 min. The treatment was conducted once a day, and 10 times were considered as one course of treatment. Two courses were needed. The changes of weight, BMI（body mass index）, F%（fat percentage） and serum leptin and NPY contents of patients in the two groups before and after treatment were observed and analyzed comparatively. Results After treatment, in male group, the weight lost（9.24±2.55） kg, BMI reduced by 4.19±2.30, F% reduced by 3.98±2.13, serum leptin level reduced by（0.66±0.23） ng/m L, and serum NPY level reduced by（18.56±2.89） pg/m L; in female group, the weight lost（6.77±2.31） kg, BMI reduced by 2.65±0.93, F% reduced by 4.98±2.30, serum leptin level reduced by（3.49±1.09） ng/m L, and serum NPY level reduced by（11.21±1.97） pg/m L. The results reduced significantly when compared with the level before treatment（P〈0.05, P〈0.01）. The loss of weight, BMI and serum NPY content in male group was more obvious than that in female group（all P〈0.01）, and the loss of F% and serum leptin content in female group was more obvious than that in male group（both P〈0.01）. Conclusion Low-frequency electroacupuncture can significantly regulate the weight, BMI, F% and serum leptin and NPY content展开更多
目的研究Motomed智能运动训练+低频电针运用于脑卒中后痉挛性偏瘫中的价值。方法将2020年3月—2021年6月厦门大学附属第一医院收住的74例脑卒中后痉挛性偏瘫患者进行分组,以随机列表法划分成两组,分别37例,联合组开展Motomed智能运动训...目的研究Motomed智能运动训练+低频电针运用于脑卒中后痉挛性偏瘫中的价值。方法将2020年3月—2021年6月厦门大学附属第一医院收住的74例脑卒中后痉挛性偏瘫患者进行分组,以随机列表法划分成两组,分别37例,联合组开展Motomed智能运动训练+低频电针,单一组实施低频电针,两周后对两组总有效率进行评价,两周后评估Ashworth痉挛分级情况,治疗前后进行神经功能缺损(national institute of health stroke scale,NIHSS)、运动功能(Fugl-Meyer)、临床症状积分、生活质量(quality of life,QOL)评分,比较两组治疗结果。结果联合组总有效率89.19%高于单一组67.57%,差异有统计学意义(P<0.05)。联合组Ashworth痉挛0级、1级发生率高出单一组,但2级、3级、4级发生率低于单一组,差异有统计学意义(P<0.05)。治疗前两组NIHSS、Fugl-Meyer评分比较差异无统计学意义(P>0.05),治疗后联合组NIHSS低于单一组,但Fugl-Meyer高出单一组,差异有统计学意义(P<0.05)。治疗前各组临床症状积分、QOL评分差异无统计学意义(P>0.05),联合组治疗后的症状积分低于单一组,但QOL较单一组升高,差异有统计学意义(P<0.05)。结论Motomed智能运动训练+低频电针的效果显著,不仅缓解症状,改善痉挛程度,增强神经功能及运动能力,也对提升生活质量有积极的作用。展开更多
文摘目的:通过观察低频电针对海洛因心理依赖小鼠海马N-甲基-D-天冬氨酸(NMDA)的亚型NMDAR1(NR1)、NM-DAR-2A(NR2A)、NMDAR-2B(NR2B)表达的影响,探讨低频电针对海洛因成瘾记忆干预作用的可能中枢机制。方法:筛选无天然位置偏爱的雄性小鼠45只,随机分为对照组、海洛因心理依赖模型组(模型组)、模型电针组(模针组),每组15只。海洛因剂量递增注射结合条件性位置偏爱(conditioned place preference,CPP)训练制作海洛因心理依赖模型,2Hz低频电针"内关"、"三阴交",点刺"四神聪"穴。采用白箱CPP值变化评价低频电针治疗效应,免疫组化方法检测海马NMDA受体亚型(NR1、NR2A、NR2B)的表达。结果:①模针组白箱CPP值较模型组明显降低(P<0.05)。②模针组小鼠NR1、NR2B阳性表达较模型组增强(P<0.05)。③模针组小鼠NR2A阳性表达较模型组降低(P<0.05)。结论:低频电针疗法能增强海洛因心理依赖小鼠海马NR1、NR2B,降低NR2A的阳性表达。这种改变可能与低频电针调节海洛因依赖的机制有关。
基金Supported by Science-technology support project of Hebei Provincial Administration of Traditional Chinese Medicine:2014015Youth fund project of Hebei University of Chinese Medicine:QNZ2014023
文摘Objective To explore the difference of low-frequency electroacupuncture in regulating serum leptin and neuropeptide Y（NPY） of male and female patients with simple obesity due to spleen deficiency and damp exuberance. Methods Eighty patients with simple obesity, including 37 males and 43 females, were included in this study. In both groups（male group and female group）, Yīnlíngquán（阴陵泉 SP 9）, Sānyīnjiāo（三阴交 SP 6）, Zúsānlǐ（足三里 ST 36）, Fēnglóng（丰隆 ST 40）, Qūchí（曲池 LI 11）, Tiānshū（天枢 ST 25）, Zhōngwǎn（中脘 CV 12）, Shuǐfēn（水分 CV 9）, Qìhǎi（气海 CV 6）, and Guānyuán（关元 CV 4）. were selected, and low-frequency electroacupuncture treatment was applied. Needles were retained for 30 min. The treatment was conducted once a day, and 10 times were considered as one course of treatment. Two courses were needed. The changes of weight, BMI（body mass index）, F%（fat percentage） and serum leptin and NPY contents of patients in the two groups before and after treatment were observed and analyzed comparatively. Results After treatment, in male group, the weight lost（9.24±2.55） kg, BMI reduced by 4.19±2.30, F% reduced by 3.98±2.13, serum leptin level reduced by（0.66±0.23） ng/m L, and serum NPY level reduced by（18.56±2.89） pg/m L; in female group, the weight lost（6.77±2.31） kg, BMI reduced by 2.65±0.93, F% reduced by 4.98±2.30, serum leptin level reduced by（3.49±1.09） ng/m L, and serum NPY level reduced by（11.21±1.97） pg/m L. The results reduced significantly when compared with the level before treatment（P〈0.05, P〈0.01）. The loss of weight, BMI and serum NPY content in male group was more obvious than that in female group（all P〈0.01）, and the loss of F% and serum leptin content in female group was more obvious than that in male group（both P〈0.01）. Conclusion Low-frequency electroacupuncture can significantly regulate the weight, BMI, F% and serum leptin and NPY content
文摘目的研究Motomed智能运动训练+低频电针运用于脑卒中后痉挛性偏瘫中的价值。方法将2020年3月—2021年6月厦门大学附属第一医院收住的74例脑卒中后痉挛性偏瘫患者进行分组,以随机列表法划分成两组,分别37例,联合组开展Motomed智能运动训练+低频电针,单一组实施低频电针,两周后对两组总有效率进行评价,两周后评估Ashworth痉挛分级情况,治疗前后进行神经功能缺损(national institute of health stroke scale,NIHSS)、运动功能(Fugl-Meyer)、临床症状积分、生活质量(quality of life,QOL)评分,比较两组治疗结果。结果联合组总有效率89.19%高于单一组67.57%,差异有统计学意义(P<0.05)。联合组Ashworth痉挛0级、1级发生率高出单一组,但2级、3级、4级发生率低于单一组,差异有统计学意义(P<0.05)。治疗前两组NIHSS、Fugl-Meyer评分比较差异无统计学意义(P>0.05),治疗后联合组NIHSS低于单一组,但Fugl-Meyer高出单一组,差异有统计学意义(P<0.05)。治疗前各组临床症状积分、QOL评分差异无统计学意义(P>0.05),联合组治疗后的症状积分低于单一组,但QOL较单一组升高,差异有统计学意义(P<0.05)。结论Motomed智能运动训练+低频电针的效果显著,不仅缓解症状,改善痉挛程度,增强神经功能及运动能力,也对提升生活质量有积极的作用。
