AIM: To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play i...AIM: To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play in acute inflammatory responses.METHODS: An intestinal ischemia-reperfusion (I/R)injury model of rats was established and rats were divided randomly into six groups: sham-operation group, 60 min ischemia/30 min reperfusion group (I60'R30'), I60'R90',I60'R150', I60'R240' and I60'R360', 9 rats each group.Two highly-sensitive radioimmunoassays for leptin and orexin-A were established and used to check the change of their concentrations in peripheral blood and central secretory tissues before and after intestinal I/R injury.RESULTS: Compared with the serum leptin level before injury, it decreased significantly in I60'R30' group and increased significantly in I60'R360' group; compared to sham-operation group after injury, serum leptin level increased significantly in I60'R360' group; compared to sham-operation group after injury, adipose leptin levels decreased significantly in I60'R30' and I60'R90' groups,while increased significantly in I60'R360' group. There was no significant difference between the expression levels of orexin-A before and after I/R injury.CONCLUSION: Leptin has a time-dependent response and orexin-A has a delayed response to acute inflammatory stimuli such as intestinal I/R injury and they may participate in metabolic disorders in injury as inflammatory cytokines.展开更多
Lipoprotein lipase (LPL) gene is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). According to cDNA of Landrace breed (GenBan...Lipoprotein lipase (LPL) gene is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). According to cDNA of Landrace breed (GenBank accession: X62984), a pair of primers was designed for amplification of intron 3. Sequencing analysis indicated that a G1200A exits in Large White breed by cloning and sequencing. The mutation can be detected by PCR-EcoT22I-RFLP. Polymorphism analysis in a resource family showed that significant difference exits in carcass traits. Pigs with AA genotype had more 6th-7th rib fat thickness (13%,P< 0.05), thorax-waist-fat thickness (11.7%,P=0.065 3), buttock fat thickness (13.1%,P=0.073 0) and average fat thickness (10.4%,P=0.050 3) than pigs with BB genotype. LPL gene showed mainly in the pattern of additive effect and all the dominant effect were not significant. The value of additive effect of 6th-7th rib fat thickness, buttock fat thickness and average fat thickness was -0.17±0.07(P< 0.05),-0.12±0.06( P< 0.05),-0.12±0.06(P< 0.05),respectively. The allelic frequency is significantly different between indigenous Chinese breeds and European breeds.展开更多
基金Supported by the National Natural Science Foundation of China,No.39970717
文摘AIM: To explore the effect of intestinal ischemia-reperfusion injury on protein levels of leptin and orexin-A in peripheral blood and their central secretory tissues and to find out the role leptin and orexin-A play in acute inflammatory responses.METHODS: An intestinal ischemia-reperfusion (I/R)injury model of rats was established and rats were divided randomly into six groups: sham-operation group, 60 min ischemia/30 min reperfusion group (I60'R30'), I60'R90',I60'R150', I60'R240' and I60'R360', 9 rats each group.Two highly-sensitive radioimmunoassays for leptin and orexin-A were established and used to check the change of their concentrations in peripheral blood and central secretory tissues before and after intestinal I/R injury.RESULTS: Compared with the serum leptin level before injury, it decreased significantly in I60'R30' group and increased significantly in I60'R360' group; compared to sham-operation group after injury, serum leptin level increased significantly in I60'R360' group; compared to sham-operation group after injury, adipose leptin levels decreased significantly in I60'R30' and I60'R90' groups,while increased significantly in I60'R360' group. There was no significant difference between the expression levels of orexin-A before and after I/R injury.CONCLUSION: Leptin has a time-dependent response and orexin-A has a delayed response to acute inflammatory stimuli such as intestinal I/R injury and they may participate in metabolic disorders in injury as inflammatory cytokines.
文摘Lipoprotein lipase (LPL) gene is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). According to cDNA of Landrace breed (GenBank accession: X62984), a pair of primers was designed for amplification of intron 3. Sequencing analysis indicated that a G1200A exits in Large White breed by cloning and sequencing. The mutation can be detected by PCR-EcoT22I-RFLP. Polymorphism analysis in a resource family showed that significant difference exits in carcass traits. Pigs with AA genotype had more 6th-7th rib fat thickness (13%,P< 0.05), thorax-waist-fat thickness (11.7%,P=0.065 3), buttock fat thickness (13.1%,P=0.073 0) and average fat thickness (10.4%,P=0.050 3) than pigs with BB genotype. LPL gene showed mainly in the pattern of additive effect and all the dominant effect were not significant. The value of additive effect of 6th-7th rib fat thickness, buttock fat thickness and average fat thickness was -0.17±0.07(P< 0.05),-0.12±0.06( P< 0.05),-0.12±0.06(P< 0.05),respectively. The allelic frequency is significantly different between indigenous Chinese breeds and European breeds.
