联合国人与生物圈计划(Man and the Biosphere Programme,简称 MAB)是联合国教科文组织(UNESCO)自1971年在全世界范围内开展的一项长期、大型国际科学合作工程。其目的在于通过全球性的科学研究、培训及信息交流,为生物圈自然资源的合...联合国人与生物圈计划(Man and the Biosphere Programme,简称 MAB)是联合国教科文组织(UNESCO)自1971年在全世界范围内开展的一项长期、大型国际科学合作工程。其目的在于通过全球性的科学研究、培训及信息交流,为生物圈自然资源的合理利用和保护提供科学依据,同时为各国的自然资源管理培养合格的专门人才。目前,全世界已有113个国家参与了这一计划,并相继成立了人与生物圈国家委员会。MAB 常设组织结构包括国际人与生物圈计划协调理事会(ICC)。展开更多
A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for ...A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA.The MAb,designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study.The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein.The sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits.The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India,1,2,15,17,18 and 23.The assay could detect BTV antigen as early as day 8 in blood.It was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood,washed RBCs,buffy coat and plasma.A total of 102 field samples from animals,suspected of being infected with BTV,were tested and 29.42% were positive.The blood samples were also amplified in cell culture which improved the sensitivity of the assay.Results confirmed that the sELISA is rapid and specific.展开更多
文摘联合国人与生物圈计划(Man and the Biosphere Programme,简称 MAB)是联合国教科文组织(UNESCO)自1971年在全世界范围内开展的一项长期、大型国际科学合作工程。其目的在于通过全球性的科学研究、培训及信息交流,为生物圈自然资源的合理利用和保护提供科学依据,同时为各国的自然资源管理培养合格的专门人才。目前,全世界已有113个国家参与了这一计划,并相继成立了人与生物圈国家委员会。MAB 常设组织结构包括国际人与生物圈计划协调理事会(ICC)。
文摘A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA.The MAb,designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study.The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein.The sELISA is based on capturing of BTV antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits.The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India,1,2,15,17,18 and 23.The assay could detect BTV antigen as early as day 8 in blood.It was also successfully applied for the detection of BTV group specific antigen in clinical samples of blood,washed RBCs,buffy coat and plasma.A total of 102 field samples from animals,suspected of being infected with BTV,were tested and 29.42% were positive.The blood samples were also amplified in cell culture which improved the sensitivity of the assay.Results confirmed that the sELISA is rapid and specific.
