目的探讨乙醇脱氢核酶ribox02与NAD^+、NADH特异性结合的位点以及结合力性质。方法通过选择性2-OH酰基化学探测法(selective 2-hydroxyl acylation analyzed by primer extension,SHAPE)探测ribox02的二级结构;利用等温滴定量热法(isoth...目的探讨乙醇脱氢核酶ribox02与NAD^+、NADH特异性结合的位点以及结合力性质。方法通过选择性2-OH酰基化学探测法(selective 2-hydroxyl acylation analyzed by primer extension,SHAPE)探测ribox02的二级结构;利用等温滴定量热法(isothermal titratio calorimetry,ITC)进一步测定ribox02与NADH、NAD^+的亲和力强度及作用力性质;通过紫外交联法精确定位NAD^+、NADH与ribox02 RNA的特异性结合位点。结果直接解析ribox02的二级结构,并发现核酶ribox02与NADH、NAD^+的相互作用力为范德华力或氢键作用,且ribox02与NADH的相互作用力较之与NAD^+更为强烈,ribox02与NADH、NAD^+的特异性结合位点为23G、24U、25U、38U、72U、73U、74U、75G和95G。结论通过ribox02的二级结构及其与NAD^+、NADH的相互作用揭示了核酶ribox02与NAD^+/NADH特异性结合位点以及结合力性质。展开更多
Quasi-solid state dye-sensitized solar cells based on chemically crosslinking with backbone polymers of poly(vinylpyridine-co-acrylonitrile) (P(VP-co-AN)) or poly(vinylimidazole-co-acrylonitrile) (P(VIM-co- AN)) and d...Quasi-solid state dye-sensitized solar cells based on chemically crosslinking with backbone polymers of poly(vinylpyridine-co-acrylonitrile) (P(VP-co-AN)) or poly(vinylimidazole-co-acrylonitrile) (P(VIM-co- AN)) and diiodide compounds of I(CH2)6I or I(CH2CH2O)nCH2CH2I solidified EC/PC/KI/I2 gel electrolytes have been fabricated. The ionic conductivities and apparent diffusion coefficients of I3? of the electro- lytes and cell performances have been investigated. Providing chemically crosslinking points, pyridine or imidazole from the backbone polymers benefited the open circuit voltage and fill factor of the cells. Consequently, the overall energy conversion efficiencies of the quasi-solid DSSCs improved over 10% even near 20% from that of the liquid electrolyte before solidification. Besides, the employing of crosslinker I(CH2CH2O)nCH2CH2I showed higher electrolytic and cell characters than that of I(CH2)6I.展开更多
文摘目的探讨乙醇脱氢核酶ribox02与NAD^+、NADH特异性结合的位点以及结合力性质。方法通过选择性2-OH酰基化学探测法(selective 2-hydroxyl acylation analyzed by primer extension,SHAPE)探测ribox02的二级结构;利用等温滴定量热法(isothermal titratio calorimetry,ITC)进一步测定ribox02与NADH、NAD^+的亲和力强度及作用力性质;通过紫外交联法精确定位NAD^+、NADH与ribox02 RNA的特异性结合位点。结果直接解析ribox02的二级结构,并发现核酶ribox02与NADH、NAD^+的相互作用力为范德华力或氢键作用,且ribox02与NADH的相互作用力较之与NAD^+更为强烈,ribox02与NADH、NAD^+的特异性结合位点为23G、24U、25U、38U、72U、73U、74U、75G和95G。结论通过ribox02的二级结构及其与NAD^+、NADH的相互作用揭示了核酶ribox02与NAD^+/NADH特异性结合位点以及结合力性质。
基金Supported by the National Research Fund for Fundamental Key Project (Grant No. 2006CB202605)the National Natural Science Foundation of China (Grant Nos. 50221201 and 50473055)
文摘Quasi-solid state dye-sensitized solar cells based on chemically crosslinking with backbone polymers of poly(vinylpyridine-co-acrylonitrile) (P(VP-co-AN)) or poly(vinylimidazole-co-acrylonitrile) (P(VIM-co- AN)) and diiodide compounds of I(CH2)6I or I(CH2CH2O)nCH2CH2I solidified EC/PC/KI/I2 gel electrolytes have been fabricated. The ionic conductivities and apparent diffusion coefficients of I3? of the electro- lytes and cell performances have been investigated. Providing chemically crosslinking points, pyridine or imidazole from the backbone polymers benefited the open circuit voltage and fill factor of the cells. Consequently, the overall energy conversion efficiencies of the quasi-solid DSSCs improved over 10% even near 20% from that of the liquid electrolyte before solidification. Besides, the employing of crosslinker I(CH2CH2O)nCH2CH2I showed higher electrolytic and cell characters than that of I(CH2)6I.
