Proteomics is one of the most active research fields in the post-genomic era. Here we briefly introduce the scientific background of the origination of proteomics and its content, research method. The new developments...Proteomics is one of the most active research fields in the post-genomic era. Here we briefly introduce the scientific background of the origination of proteomics and its content, research method. The new developments of proteomics at the levels of individual plants, tissues, organs and organells, as well as its applications in the area of plant genetic diversity, mutant characterization, and plant physiology, etc are reviewed. At last, the challenge and prospect of proteomics are discussed.展开更多
AIM: To isolate and identify differentially expressed proteins between cancer and normal tissues of gastric cancer by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flig...AIM: To isolate and identify differentially expressed proteins between cancer and normal tissues of gastric cancer by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). METHODS: Soluble fraction proteins of gastric cancer tissues and paired normal tissues were separated by 2-DE. The differentially expressed proteins were selected and identified by MALDI-TOF-MS and database search. RESULTS: 2-DE profiles with high resolution and reproducibility were obtained. Twenty-three protein spots were excised from sliver staining gel and digested in gel by trypsin, in which fifteen protein spots were identified successfully. Among the identified proteins, there were ten over-expressed and five under-expressed proteins in stomach cancer tissues compared with normal tissues. CONCLUSION: In this study, the well-resolved, reproducible 2-DE patterns of human gastric cancer tissue and paired normal tissue were established and optimized and certain differentially-expressed proteins were identified. The combined use of 2-DE and MS provides an effective approach to screen for potential tumor markers.展开更多
目的:分离胃癌蛋白质组,找出胃癌组织的差异蛋白质点,为进一步鉴定胃癌的差异蛋白质打下基础。方法:利用二维聚丙烯酰胺凝胶电泳(two-dimensional polyacrylamide gel electrophoresis,2-DE)分离胃癌组和正常胃粘膜对照组总蛋白质,并通...目的:分离胃癌蛋白质组,找出胃癌组织的差异蛋白质点,为进一步鉴定胃癌的差异蛋白质打下基础。方法:利用二维聚丙烯酰胺凝胶电泳(two-dimensional polyacrylamide gel electrophoresis,2-DE)分离胃癌组和正常胃粘膜对照组总蛋白质,并通过优化的计算机图像分析软件分析电泳图谱。结果:在胃癌组织中点检测共有蛋白质点1 147个,正常胃粘膜组织中点检测共有蛋白质点1 079个,发现两者间共有164个差异蛋白质点,其中41个点仅在胃癌组织中表达,27个点仅在正常胃粘膜中表达,39个点在胃癌组织中高表达,57个点在胃癌组织中低表达。结论:通过优化的双向凝胶电泳和图像分析技术对胃癌蛋白质组进行研究,获得了胃癌蛋白质组与正常对照组的差异蛋白质点。为进一步利用质谱分析技术做差异蛋白质鉴定奠定了基础。展开更多
目的初步分析稳定性心绞痛(SAP)患者血清中差异蛋白质的表达。方法收集年龄与性别相匹配的50例SAP初诊患者与50例体检健康者的血清,分别混合血清后去除IgG和清蛋白,进行PAGE并观察去除效率;进行固相pH梯度SDS-PAGE二维凝胶电泳,对凝胶...目的初步分析稳定性心绞痛(SAP)患者血清中差异蛋白质的表达。方法收集年龄与性别相匹配的50例SAP初诊患者与50例体检健康者的血清,分别混合血清后去除IgG和清蛋白,进行PAGE并观察去除效率;进行固相pH梯度SDS-PAGE二维凝胶电泳,对凝胶进行银染后用Image Master 5.00软件分析;对差异点用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术进行测定。结果去除IgG和清蛋白前后的SDS-PAGE电泳图效果良好,IgG和清蛋白去除率均>80%;分析获得的2个差异点经质谱分析,间-α-胰蛋白酶抑制剂家族重链相关蛋白(inter-α-trypsin inhibitor family heavy-chainrelated protein,IHRP)、锌指蛋白A20(zinc finger protein A20,A20)在SAP患者血清中表达而在健康人血清中无表达。结论IHRP和A20可能在SAP发病机制中起一定作用。展开更多
文摘Proteomics is one of the most active research fields in the post-genomic era. Here we briefly introduce the scientific background of the origination of proteomics and its content, research method. The new developments of proteomics at the levels of individual plants, tissues, organs and organells, as well as its applications in the area of plant genetic diversity, mutant characterization, and plant physiology, etc are reviewed. At last, the challenge and prospect of proteomics are discussed.
基金National High Technology Research and Development Program of China (863 Program) No. 2006AA 02A301 and No. 2007AA02Z179Science and Technology Commission of Shanghai Municipality, No. 07jc14041National Key Fundamental Research 973 Project, No. 2002CB13700
文摘AIM: To isolate and identify differentially expressed proteins between cancer and normal tissues of gastric cancer by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). METHODS: Soluble fraction proteins of gastric cancer tissues and paired normal tissues were separated by 2-DE. The differentially expressed proteins were selected and identified by MALDI-TOF-MS and database search. RESULTS: 2-DE profiles with high resolution and reproducibility were obtained. Twenty-three protein spots were excised from sliver staining gel and digested in gel by trypsin, in which fifteen protein spots were identified successfully. Among the identified proteins, there were ten over-expressed and five under-expressed proteins in stomach cancer tissues compared with normal tissues. CONCLUSION: In this study, the well-resolved, reproducible 2-DE patterns of human gastric cancer tissue and paired normal tissue were established and optimized and certain differentially-expressed proteins were identified. The combined use of 2-DE and MS provides an effective approach to screen for potential tumor markers.
文摘目的初步分析稳定性心绞痛(SAP)患者血清中差异蛋白质的表达。方法收集年龄与性别相匹配的50例SAP初诊患者与50例体检健康者的血清,分别混合血清后去除IgG和清蛋白,进行PAGE并观察去除效率;进行固相pH梯度SDS-PAGE二维凝胶电泳,对凝胶进行银染后用Image Master 5.00软件分析;对差异点用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术进行测定。结果去除IgG和清蛋白前后的SDS-PAGE电泳图效果良好,IgG和清蛋白去除率均>80%;分析获得的2个差异点经质谱分析,间-α-胰蛋白酶抑制剂家族重链相关蛋白(inter-α-trypsin inhibitor family heavy-chainrelated protein,IHRP)、锌指蛋白A20(zinc finger protein A20,A20)在SAP患者血清中表达而在健康人血清中无表达。结论IHRP和A20可能在SAP发病机制中起一定作用。
