目的 :研究食管癌及食管上皮人类白细胞抗原 (HLA A ,B ,C)的抗原表达及探索该抗原丢失的分子机理。方法 :采用抗HLA A ,B ,C单抗 ,以免疫组化法及甲基化PCR检测HLA A ,B ,C抗原在食管癌及食管上皮的分布及HLA A ,B ,C基因甲基化状况。...目的 :研究食管癌及食管上皮人类白细胞抗原 (HLA A ,B ,C)的抗原表达及探索该抗原丢失的分子机理。方法 :采用抗HLA A ,B ,C单抗 ,以免疫组化法及甲基化PCR检测HLA A ,B ,C抗原在食管癌及食管上皮的分布及HLA A ,B ,C基因甲基化状况。结果 :31例食管癌中 2 7例无HLA A ,B ,C抗原表达 ,正常及异常增生的食管上皮均可见该抗原的阳性表达 ;45 2 %的食管癌HLA A ,B ,C基因甲基化 ,食管上皮该基因无甲基化。结论 :食管癌中HLA A ,B ,C抗原大部分丢失 。展开更多
Inflammatory bowel disease （IBD） includes two similar yet distinct conditions called ulcerative colitis （UC） and Crohn＇s disease （CD）. These diseases affect the digestive system and cause the inflammation of in...Inflammatory bowel disease （IBD） includes two similar yet distinct conditions called ulcerative colitis （UC） and Crohn＇s disease （CD）. These diseases affect the digestive system and cause the inflammation of intestinal tissue, form sores and bleed easily. Most children with IBD are diagnosed in late childhood and adolescence. However, both UC and CD have been reported as early as in infancy. Most information pertaining to the epidemiology of IBD is based upon adult studies. Symptoms include abdominal pain, cramping, fatigue and diarrhea. Genetic factors play a significant role in determining IBD susceptibility. Epidemiological data support a genetic contribution to the pathogenesis of IBD. Recently, numerous new genes have been identified as being involved in the genetic susceptibility to IBD： TNF- 308A, CARD15 （NOD2）, MIF-173, N-acetyltransferase 2 （NAT2）, NKG2D （natural killer cell 2D）, STAT6 （signal transducer and activator of transcription 6）, CTLA-4 （cytotoxic T lymphocyte antigen-4）, MICA-MICB （major histocompatibility complex A and B）, HLA-DRB1, HLA class-Ⅱ, IL-18, IL-4, MICA-A5, CD14, TI R4, Fas-670, p53 and NF-kB. The characterization of these novel genes has the potential to identify therapeutic agents and aid clinical assessment of phenotype and prognosis in patients with IBD （UC and CD）.展开更多
AIM:To demonstrate whether class I MHC molecule,transporter associated with antigen processing (TAP), and heat-shock proteion70 (HSP70) expressed in liver cancer cells before the design and construction of CTL vaccine...AIM:To demonstrate whether class I MHC molecule,transporter associated with antigen processing (TAP), and heat-shock proteion70 (HSP70) expressed in liver cancer cells before the design and construction of CTL vaccine against hepatocellular carcinoma (HCC).METHODS: We studied 30 HCC specimens by labeled streptavidin biotin (LSAB) method of immunohistochemistry.RESULTS: The results showed that the majority of HCC cells investigated naturally expressed class I MHC and TAP,which were different from other tumor cells. Furthermore,we found that HSP70 expressed not only in cellularcytoplasm, but also on the cell surface in HCC.CONCLUSION: Our findings indicate that our understanding about immune escape mechanisms employed by HCC cells may be further improved, It is important to design and construct CTL vaccine against HCC.展开更多
基金Supported by The Science Foundation of Health Bureau of Shaanxi Province,China,No.04D26
文摘Inflammatory bowel disease （IBD） includes two similar yet distinct conditions called ulcerative colitis （UC） and Crohn＇s disease （CD）. These diseases affect the digestive system and cause the inflammation of intestinal tissue, form sores and bleed easily. Most children with IBD are diagnosed in late childhood and adolescence. However, both UC and CD have been reported as early as in infancy. Most information pertaining to the epidemiology of IBD is based upon adult studies. Symptoms include abdominal pain, cramping, fatigue and diarrhea. Genetic factors play a significant role in determining IBD susceptibility. Epidemiological data support a genetic contribution to the pathogenesis of IBD. Recently, numerous new genes have been identified as being involved in the genetic susceptibility to IBD： TNF- 308A, CARD15 （NOD2）, MIF-173, N-acetyltransferase 2 （NAT2）, NKG2D （natural killer cell 2D）, STAT6 （signal transducer and activator of transcription 6）, CTLA-4 （cytotoxic T lymphocyte antigen-4）, MICA-MICB （major histocompatibility complex A and B）, HLA-DRB1, HLA class-Ⅱ, IL-18, IL-4, MICA-A5, CD14, TI R4, Fas-670, p53 and NF-kB. The characterization of these novel genes has the potential to identify therapeutic agents and aid clinical assessment of phenotype and prognosis in patients with IBD （UC and CD）.
基金the National Natural Science Foundation of China,No.30070855
文摘AIM:To demonstrate whether class I MHC molecule,transporter associated with antigen processing (TAP), and heat-shock proteion70 (HSP70) expressed in liver cancer cells before the design and construction of CTL vaccine against hepatocellular carcinoma (HCC).METHODS: We studied 30 HCC specimens by labeled streptavidin biotin (LSAB) method of immunohistochemistry.RESULTS: The results showed that the majority of HCC cells investigated naturally expressed class I MHC and TAP,which were different from other tumor cells. Furthermore,we found that HSP70 expressed not only in cellularcytoplasm, but also on the cell surface in HCC.CONCLUSION: Our findings indicate that our understanding about immune escape mechanisms employed by HCC cells may be further improved, It is important to design and construct CTL vaccine against HCC.
