Triple-negative breast cancer （TNBC）, characterized by the lack of expression of the estrogen receptor, the progesterone receptor, and the human epidermal growth factor receptor 2, is an aggressive form of cancer th...Triple-negative breast cancer （TNBC）, characterized by the lack of expression of the estrogen receptor, the progesterone receptor, and the human epidermal growth factor receptor 2, is an aggressive form of cancer that conveys unpredictable and poor prognosis due to limited treatment options and lack of effective targeted therapies. Wnt/β-catenin signaling is hyperactivated in TNBC, which promotes the progression of TNBC. However, the molecular mechanism of Wnt/β-catenin activation in TNBC remains unknown. Here, we report the drastic overexpression of miR-221/222 in all of four TNBC cell lines and TNBC primary tumor samples from patients. Furthermore, we demonstrate by both ex vivo and xenograft experiments that inhibiting miR-221/222 expression in a TNBC cell line （MDA-MB-231） suppresses its proliferation, viability, epithelial-to-mesenchymal transition, and migration; whereas expressing miR-221/222 in a non-TNBC line （MCF7） promotes all of the above cancer properties. miR-221/222 achieve so by directly repressing multiple negative regulators of the Wnt/β-catenin signaling pathway, including WIF1, SFRP2, DKK2, and AXIN2, to activate the pathway. Notably, the level of miR-221/222 expression is inversely correlated whereas that of WIF1, DKK2, SFRP2, and AXIN2 expression is positively correlated with the patient survival. Last, we show that anti-miR-221/222 significantly increases apoptotic cells with tamoxifen/Wnt3a treatment but not with cyclophosphamide/Wnt3a treatment. These results demonstrate that miR-221/222 activate the Wnt/β-catenin signaling to promote the aggressiveness and TNBC properties of breast cancers, and thus reveal a new prospect for TNBC treatment.展开更多
利用专利文献数据识别技术领域的技术主题演化发展路径并分析其发展趋势,对于科技界、企业界进行专利技术创新具有重要的意义。首先,使用Open IE 5.1进行SAO(subject-action-object)三元组抽取,基于LDA(latent Dirichlet allocation)模...利用专利文献数据识别技术领域的技术主题演化发展路径并分析其发展趋势,对于科技界、企业界进行专利技术创新具有重要的意义。首先,使用Open IE 5.1进行SAO(subject-action-object)三元组抽取,基于LDA(latent Dirichlet allocation)模型进行主题识别,根据TRIZ技术创新思想,基于action语义词典将技术主题划分到四个维度;然后,通过计算SAO三元组之间的相似度来测度技术主题之间的语义关联构建技术主题创新演化路径,并利用可视化技术构建技术主题创新演化路径可视化图谱,利用该图谱辅助分析技术主题演化脉络及其发展趋势。最后,通过石墨烯超级电容器(集流体)领域的实证,对该领域的技术问题(problem to problem,P-P)主题、技术功能(solution to solution,S-S)主题、解决方案(problem to solution,P-S)主题和技术效果(solution to problem,S-P)主题的创新演化路径进行解读分析,验证了本研究提出方法的可行性和有效性。展开更多
提出了一种对语义网上的本体进行检索和排序的新方法ARRO(an Approach for Retrieval and Ranking for the On-tology),其核心思想是通过对本体进行解析产生逻辑三元组.再在三元组的基础上进行逻辑推理,形成概念的逻辑视图,然后通过排...提出了一种对语义网上的本体进行检索和排序的新方法ARRO(an Approach for Retrieval and Ranking for the On-tology),其核心思想是通过对本体进行解析产生逻辑三元组.再在三元组的基础上进行逻辑推理,形成概念的逻辑视图,然后通过排序公式对相关本体进行检索和排序.这种通过逻辑视图和三元组对本体进行检索和排序的方法可以有效的进行逻辑推理,并提高检索效率,从而解决在传统的基于关键字的信息检索中只能从句法上对关键字进行分析,无法将推理和检索相互结合,互相促进的问题.本文对ARRO进行了测试,结果验证了其实用性和有效性.展开更多
基金This research was funded by the Shanghai Institute for Advanced Immunochmical Studies at ShanghaiTech University to H.L.S.L. was supported by the National Natural Science Foundation of China （81772798）. Z.L. was supported by the School of Life Science and Technology at ShanghaiTech University.
文摘Triple-negative breast cancer （TNBC）, characterized by the lack of expression of the estrogen receptor, the progesterone receptor, and the human epidermal growth factor receptor 2, is an aggressive form of cancer that conveys unpredictable and poor prognosis due to limited treatment options and lack of effective targeted therapies. Wnt/β-catenin signaling is hyperactivated in TNBC, which promotes the progression of TNBC. However, the molecular mechanism of Wnt/β-catenin activation in TNBC remains unknown. Here, we report the drastic overexpression of miR-221/222 in all of four TNBC cell lines and TNBC primary tumor samples from patients. Furthermore, we demonstrate by both ex vivo and xenograft experiments that inhibiting miR-221/222 expression in a TNBC cell line （MDA-MB-231） suppresses its proliferation, viability, epithelial-to-mesenchymal transition, and migration; whereas expressing miR-221/222 in a non-TNBC line （MCF7） promotes all of the above cancer properties. miR-221/222 achieve so by directly repressing multiple negative regulators of the Wnt/β-catenin signaling pathway, including WIF1, SFRP2, DKK2, and AXIN2, to activate the pathway. Notably, the level of miR-221/222 expression is inversely correlated whereas that of WIF1, DKK2, SFRP2, and AXIN2 expression is positively correlated with the patient survival. Last, we show that anti-miR-221/222 significantly increases apoptotic cells with tamoxifen/Wnt3a treatment but not with cyclophosphamide/Wnt3a treatment. These results demonstrate that miR-221/222 activate the Wnt/β-catenin signaling to promote the aggressiveness and TNBC properties of breast cancers, and thus reveal a new prospect for TNBC treatment.
文摘利用专利文献数据识别技术领域的技术主题演化发展路径并分析其发展趋势,对于科技界、企业界进行专利技术创新具有重要的意义。首先,使用Open IE 5.1进行SAO(subject-action-object)三元组抽取,基于LDA(latent Dirichlet allocation)模型进行主题识别,根据TRIZ技术创新思想,基于action语义词典将技术主题划分到四个维度;然后,通过计算SAO三元组之间的相似度来测度技术主题之间的语义关联构建技术主题创新演化路径,并利用可视化技术构建技术主题创新演化路径可视化图谱,利用该图谱辅助分析技术主题演化脉络及其发展趋势。最后,通过石墨烯超级电容器(集流体)领域的实证,对该领域的技术问题(problem to problem,P-P)主题、技术功能(solution to solution,S-S)主题、解决方案(problem to solution,P-S)主题和技术效果(solution to problem,S-P)主题的创新演化路径进行解读分析,验证了本研究提出方法的可行性和有效性。
文摘提出了一种对语义网上的本体进行检索和排序的新方法ARRO(an Approach for Retrieval and Ranking for the On-tology),其核心思想是通过对本体进行解析产生逻辑三元组.再在三元组的基础上进行逻辑推理,形成概念的逻辑视图,然后通过排序公式对相关本体进行检索和排序.这种通过逻辑视图和三元组对本体进行检索和排序的方法可以有效的进行逻辑推理,并提高检索效率,从而解决在传统的基于关键字的信息检索中只能从句法上对关键字进行分析,无法将推理和检索相互结合,互相促进的问题.本文对ARRO进行了测试,结果验证了其实用性和有效性.
