Phosphoaspartate is one of the major components of eukaryotes and prokaryotic two-component signaling pathways,and it communicates the signal from the sensor of histidine kinase,through the response regulator,to the D...Phosphoaspartate is one of the major components of eukaryotes and prokaryotic two-component signaling pathways,and it communicates the signal from the sensor of histidine kinase,through the response regulator,to the DNA alongside transcription features and initiates the transcription of correct response genes.Thus,the prediction of phosphoaspartate sites is critical,and its experimental identification can be expensive,time-consuming,and tedious.For this purpose,we propose iPhosD-PseAAC,a new computational model for predicting phosphoaspartate sites in a particular protein sequence using Chou’s 5-steps rues:(1)Benchmark dataset.(2)The feature extraction techniques such as pseudo amino acid composition(PseAAC),statistical moments,and position relative features.(3)For the classification,artificial neural network AAN will be used.(4)In this step,10-fold cross-validation and self-consistency testing will be used for validation.For self-consistency testing,100%Acc is achieved,whereas,for 10-fold crossvalidation 95.14%Acc,95.58%Sn,94.70%Sp and 0.95 MCC are observed.(5).The final step is the development of a user-friendly web server for the ease of users.Thus,the iPhosD-PseAAC is the first and novel predictor for accurate and efficient identification of phosphoaspartate sites.展开更多
基金the Deanship of Scientific Research(DSR)at King Abdulaziz University,Jeddah,https://dsr.kau.edu.sa/Default-305-EN under Grant No.G:136-611-1441.
文摘Phosphoaspartate is one of the major components of eukaryotes and prokaryotic two-component signaling pathways,and it communicates the signal from the sensor of histidine kinase,through the response regulator,to the DNA alongside transcription features and initiates the transcription of correct response genes.Thus,the prediction of phosphoaspartate sites is critical,and its experimental identification can be expensive,time-consuming,and tedious.For this purpose,we propose iPhosD-PseAAC,a new computational model for predicting phosphoaspartate sites in a particular protein sequence using Chou’s 5-steps rues:(1)Benchmark dataset.(2)The feature extraction techniques such as pseudo amino acid composition(PseAAC),statistical moments,and position relative features.(3)For the classification,artificial neural network AAN will be used.(4)In this step,10-fold cross-validation and self-consistency testing will be used for validation.For self-consistency testing,100%Acc is achieved,whereas,for 10-fold crossvalidation 95.14%Acc,95.58%Sn,94.70%Sp and 0.95 MCC are observed.(5).The final step is the development of a user-friendly web server for the ease of users.Thus,the iPhosD-PseAAC is the first and novel predictor for accurate and efficient identification of phosphoaspartate sites.
