11月18日,华为与微软在京共同推出U-SYS Work Space企业统一通信解决方案。该方案汇聚了华为在下一代通信网络(NGN)和微软在企业办公系统两方面的优势,为企业提供更加高效的通信方案,并以此推进下一代通信网络的商用进程。这是华为下一...11月18日,华为与微软在京共同推出U-SYS Work Space企业统一通信解决方案。该方案汇聚了华为在下一代通信网络(NGN)和微软在企业办公系统两方面的优势,为企业提供更加高效的通信方案,并以此推进下一代通信网络的商用进程。这是华为下一代网络产品确立业界领先地位之后,在企业通信应用领域的又一重大突破。展开更多
作为一台采用标准电压版高频Pentium M CPU的光驱内置型笔记本电脑，M5N的特点是轻、薄、靓。M5N的优秀之处，在于它面向的用户的广泛针对性，它适用于大部分的笔记本电脑消费者，无论家用或是商用。和之前的S5N相比，M5N在各个方面都...作为一台采用标准电压版高频Pentium M CPU的光驱内置型笔记本电脑，M5N的特点是轻、薄、靓。M5N的优秀之处，在于它面向的用户的广泛针对性，它适用于大部分的笔记本电脑消费者，无论家用或是商用。和之前的S5N相比，M5N在各个方面都迈出了一小步；综合来讲，我们又可以称其在同级别产品中走出了一大步。展开更多
建立一种便捷、灵敏的检测方法,即逆转录环介导等温核酸扩增技术(RT-LAMP)用于H5N1亚型禽流感病毒基因检测。该技术使用特异对应于靶序列中8个基因区段的6条特异引物,在等温条件下进行核酸扩增反应。对51份实验感染动物及病毒培养标本的...建立一种便捷、灵敏的检测方法,即逆转录环介导等温核酸扩增技术(RT-LAMP)用于H5N1亚型禽流感病毒基因检测。该技术使用特异对应于靶序列中8个基因区段的6条特异引物,在等温条件下进行核酸扩增反应。对51份实验感染动物及病毒培养标本的H5N1亚型禽流感病毒的HA、NA基因区进行了RT-LAMP检测,并以SYBR Green I为反应指示剂进行了逆转录环介导等温核酸扩增技术,对该反应进行实时监控,经对扩增产物做内切酶验证和测序分析,证明RT-LAMP技术的特异性;同时,用10倍系列稀释的RNA样品对该检测方法的灵敏度进行了测试。结果显示:利用RT-LAMP技术成功检测到H5N1禽流感病毒的HA、NA基因区,且RT-LAMP与Real-time PCR结果呈现很好的一致性。此方法的灵敏度可达到能检测10个拷贝RNA分子水平。因此,RT-LAMP技术应用于H5N1亚型禽流感病毒的快速检测是一种可行的方法。展开更多
H5N1 highly pathogenic avian influenza virus was first detected in a goose in Guangdong Province of China in 1996. Multiple genotypes of H5N1 viruses have been identified from apparently healthy waterfowl since 1999. ...H5N1 highly pathogenic avian influenza virus was first detected in a goose in Guangdong Province of China in 1996. Multiple genotypes of H5N1 viruses have been identified from apparently healthy waterfowl since 1999. In the years 2004-2008, over 100 outbreaks in domestic poultry occurred in 23 provinces and caused severe economic damage to the poultry industry in China. Beginning from 2004, a culling plus vaccination strategy has been implemented for the control of epidemics. Since then, over 35420000 poultry have been depopulated, and over 55 billion doses of the different vaccines have been used to control the outbreaks. Although it is logistically impossible to vaccinate every single bird in China due to the large poultry population and the complicated rearing styles, there is no doubt that the increased vaccination coverage has resulted in decreased disease epidemic and environmental virus loading. The experience in China suggests that vaccination has played an important role in the protection of poultry from H5N1 virus infection, the reduction of virus load in the environment, and the prevention of H5N1 virus transmission from poultry to humans.展开更多
文摘11月18日,华为与微软在京共同推出U-SYS Work Space企业统一通信解决方案。该方案汇聚了华为在下一代通信网络(NGN)和微软在企业办公系统两方面的优势,为企业提供更加高效的通信方案,并以此推进下一代通信网络的商用进程。这是华为下一代网络产品确立业界领先地位之后,在企业通信应用领域的又一重大突破。
文摘作为一台采用标准电压版高频Pentium M CPU的光驱内置型笔记本电脑，M5N的特点是轻、薄、靓。M5N的优秀之处，在于它面向的用户的广泛针对性，它适用于大部分的笔记本电脑消费者，无论家用或是商用。和之前的S5N相比，M5N在各个方面都迈出了一小步；综合来讲，我们又可以称其在同级别产品中走出了一大步。
文摘建立一种便捷、灵敏的检测方法,即逆转录环介导等温核酸扩增技术(RT-LAMP)用于H5N1亚型禽流感病毒基因检测。该技术使用特异对应于靶序列中8个基因区段的6条特异引物,在等温条件下进行核酸扩增反应。对51份实验感染动物及病毒培养标本的H5N1亚型禽流感病毒的HA、NA基因区进行了RT-LAMP检测,并以SYBR Green I为反应指示剂进行了逆转录环介导等温核酸扩增技术,对该反应进行实时监控,经对扩增产物做内切酶验证和测序分析,证明RT-LAMP技术的特异性;同时,用10倍系列稀释的RNA样品对该检测方法的灵敏度进行了测试。结果显示:利用RT-LAMP技术成功检测到H5N1禽流感病毒的HA、NA基因区,且RT-LAMP与Real-time PCR结果呈现很好的一致性。此方法的灵敏度可达到能检测10个拷贝RNA分子水平。因此,RT-LAMP技术应用于H5N1亚型禽流感病毒的快速检测是一种可行的方法。
基金Supported by the Key Animal Infectious Disease Control Program of the Ministry of Agriculture, the Chinese National S&T Plan(Grant No. 2004BA519A-57)National Key Basic Research and Development Program of China (Grant Nos: 2005CB523005, 2005CB523200).
文摘H5N1 highly pathogenic avian influenza virus was first detected in a goose in Guangdong Province of China in 1996. Multiple genotypes of H5N1 viruses have been identified from apparently healthy waterfowl since 1999. In the years 2004-2008, over 100 outbreaks in domestic poultry occurred in 23 provinces and caused severe economic damage to the poultry industry in China. Beginning from 2004, a culling plus vaccination strategy has been implemented for the control of epidemics. Since then, over 35420000 poultry have been depopulated, and over 55 billion doses of the different vaccines have been used to control the outbreaks. Although it is logistically impossible to vaccinate every single bird in China due to the large poultry population and the complicated rearing styles, there is no doubt that the increased vaccination coverage has resulted in decreased disease epidemic and environmental virus loading. The experience in China suggests that vaccination has played an important role in the protection of poultry from H5N1 virus infection, the reduction of virus load in the environment, and the prevention of H5N1 virus transmission from poultry to humans.
基金supported by The Research Grants Council of The HongKong SAR Government (7488/05M) the Research Fund for the Control of Infectious Diseases of the Health, Welfare and Food Bureauof the Hong Kong SAR Government, the Li Ka Shing Foundation+1 种基金the Providence Foundation in memory of The late Dr. Lui Hac Minhgrants from The National Natural Science Foundation of China(30571674, 30771988)~~
