目的验证钴60-γ射线辐照对动物源性膜材中模拟感染指示病毒的灭活效果。方法将3个连续批次的动物源性膜材中间品,按1∶10(m/V)加入预先测定滴度的CVB3、PRV、BVDV和PPV病毒。设立未经辐照的病毒为对照组,试验组经25 k Gy钴60-γ射线辐...目的验证钴60-γ射线辐照对动物源性膜材中模拟感染指示病毒的灭活效果。方法将3个连续批次的动物源性膜材中间品,按1∶10(m/V)加入预先测定滴度的CVB3、PRV、BVDV和PPV病毒。设立未经辐照的病毒为对照组,试验组经25 k Gy钴60-γ射线辐照后,提取浸提液,采用96孔板细胞病变(CPE)法观察各梯度稀释液的细胞病变情况,按照Reed-Muench法计算对照组和试验组中4种指示病毒的lg TCID50/0.1 ml,并对比辐照前后病毒滴度的变化情况。结果经25 k Gy钴60-γ射线辐照预先感染病毒的动物源性膜材后,实验结果显示4种指示病毒均不能使宿主细胞出现病变,各病毒滴度对数值变化均>4 logs,符合国家相关法规对动物源性材料病毒灭活效果的要求。结论25 k Gy钴60-γ射线辐照可以作为灭活动物源性膜材中潜在病毒的有效方法。展开更多
The dynamic viscoelastic properties of bamboo were tested by dynamic mechanics analyzer (DMA),and the measurements were done in the temperature range of 40℃ to 300℃at frequencies of 3 Hz.The results showed that with...The dynamic viscoelastic properties of bamboo were tested by dynamic mechanics analyzer (DMA),and the measurements were done in the temperature range of 40℃ to 300℃at frequencies of 3 Hz.The results showed that with the increase of irradiation dose,the decrease of storage modulus with increasing temperature became more dramatically.The decrease extent of storage modulus was the lowest for non-irradiated bamboo.Two mechanical relaxation processes were observed for all kinds of irradiated bamboo.The a relaxation process in lower temperature range was due to glass transition of hemicellulose and login,while the β relaxation process occurred in higher temperature range was based on glass transition of cellulose.The loss peak strength ofαrelaxation process went up as the irradiation dose increase,while the loss peak strength of β relaxation process became up first and then down,which was caused by the chemical reaction of the chief composition of bamboo.展开更多
文摘目的验证钴60-γ射线辐照对动物源性膜材中模拟感染指示病毒的灭活效果。方法将3个连续批次的动物源性膜材中间品,按1∶10(m/V)加入预先测定滴度的CVB3、PRV、BVDV和PPV病毒。设立未经辐照的病毒为对照组,试验组经25 k Gy钴60-γ射线辐照后,提取浸提液,采用96孔板细胞病变(CPE)法观察各梯度稀释液的细胞病变情况,按照Reed-Muench法计算对照组和试验组中4种指示病毒的lg TCID50/0.1 ml,并对比辐照前后病毒滴度的变化情况。结果经25 k Gy钴60-γ射线辐照预先感染病毒的动物源性膜材后,实验结果显示4种指示病毒均不能使宿主细胞出现病变,各病毒滴度对数值变化均>4 logs,符合国家相关法规对动物源性材料病毒灭活效果的要求。结论25 k Gy钴60-γ射线辐照可以作为灭活动物源性膜材中潜在病毒的有效方法。
文摘The dynamic viscoelastic properties of bamboo were tested by dynamic mechanics analyzer (DMA),and the measurements were done in the temperature range of 40℃ to 300℃at frequencies of 3 Hz.The results showed that with the increase of irradiation dose,the decrease of storage modulus with increasing temperature became more dramatically.The decrease extent of storage modulus was the lowest for non-irradiated bamboo.Two mechanical relaxation processes were observed for all kinds of irradiated bamboo.The a relaxation process in lower temperature range was due to glass transition of hemicellulose and login,while the β relaxation process occurred in higher temperature range was based on glass transition of cellulose.The loss peak strength ofαrelaxation process went up as the irradiation dose increase,while the loss peak strength of β relaxation process became up first and then down,which was caused by the chemical reaction of the chief composition of bamboo.
