AIM: To study the expression of cyclooxygenase-2 (COX-2)gene in gastric cancer and the relationship between COX-2expression and clinicopathologic features of gastric cancer.METHODS: With reference to the expression of...AIM: To study the expression of cyclooxygenase-2 (COX-2)gene in gastric cancer and the relationship between COX-2expression and clinicopathologic features of gastric cancer.METHODS: With reference to the expression of β-actin gene,COX-2 mRNA level was examined in cancerous tissues andadjacent noncancerous mucosa from 33 patients bysemiquantitative reverse transcription- polymerase chainreaction (RT-PCR). Quantitation of relative band Adj volumecounts was performed using molecular Analyst for windowssoftware. The COX-2 index was determined from the band Adjvolume counts ratio of COX-2 to constitutively expressed actin.RESULTS: The COX-2 index in gastric carcinoma wassignificantly higher than that in normal mucosa (0.5966±0.2659vs 0.2979+0.171, u=5.4309, P＜0.01). Significantly higherexpression of COX-2 mRNA was also observed in patientswith lymph node involvement than that in those without(0.6775±0.2486 vs 0.4105±0.2182, t=2.9341, P＜0.01).Furthermore, the staging in the UICC TNM classificationsignificantly correlated with COX-2 overexpression (F＝3.656,P＜0.05), the COX-2 index in stage Ⅲ and IV was significantlyhigher than those in stage Ⅰ and Ⅱ(q=3.2728 and q=3.4906, P＜0.05). The COX-2 index showed no correlationwith patient's age, sex, blood group, tumor location, grosstyping, depth of invasion, differentiation, and the greatesttumor dimension (P＞0.05).CONCLUSION: Expression of COX-2 mRNA in gastriccarcinoma was significantly higher, which may enhancelymphatic metastasis in patients with gastric carcinoma. Thestaging in the UICC TNM classification was significantlycorrelated with COX-2 over-expression. COX-2 may contributeto progression of tumor in human gastric adenocarcinoma.展开更多
AIM: To elucidate the expression of E-cadherin and β-catenincorrelating with its clinical outcome in patients withesophageal squamous cell carcinoma (ESCC), by analyzingtheir interrelationship with clinicopathologica...AIM: To elucidate the expression of E-cadherin and β-catenincorrelating with its clinical outcome in patients withesophageal squamous cell carcinoma (ESCC), by analyzingtheir interrelationship with clinicopathological variables andtheir effects on progress and prognosis.METHODS: Expression of E-cadherin and β-catenin wasdetermined by SP immunohistochemical technique inpatients with ESCC consecutively, their correlation withclinical characteristics was evaluated and analyzed bymultivariate analysis.RESULTS: The rate of expression of E-cadherin decreasedto 66.03 % (70/106) in ESCC and the protein level wasnegative correlated with histologic grade, tumor size, clinicalstaging, lymph node metastasis and venous invasion.Whereas the expression rate of β-catenin was reduced to69.8 % (74/106) and the level of protein expressioncorrelated only with histologic grade. There obviously existedinverse correlation between level of E-cadherin protein andsurvival, especially in .stage I, IIa, IIb (P=0.0033), Patientswith low-expressing tumors for β-catenin and non-expressingtumors for E-cadherin/β-catenin had lower survival periodthan those with normal-expressing ones (P=0.0501 andP=0.0080, respectively). Patients with diminished expressionof E-cadherin as grade Ⅱ or Ⅲ had shorter survival periodthan those with normally expressing and grade Ⅰ, nosignificance existed between grade I and grade Ⅱ or Ⅲwith respect to different status of E-cadherin expression.Furthermore, Correlation analysis showed level of E-cadherincorrelated with that of β-catenin (P=0.005). Cox proportionalhazards model analysis suggested downregulation of E-cadherin was an important factor indicating poor prognosis.CONCLUSION: As a probable independent prognosticfactor, it correlates with overall and disease free survivalperiod, expression of E-cadherin but not β-catenin maypredict prognosis in patients with ESCC.展开更多
目的:探讨上皮钙黏附蛋白(E-cad)、β-连接素(β-cat)和基质金属蛋白酶-7(MMP-7)在遗传性非息肉病性大肠癌(HNPCC)和散发性大肠癌(sporadic CRC)的表达及与遗传性非息肉病性大肠癌(HNPCC)侵袭转移间的关系.方法:应用免疫组化SP法检测HNP...目的:探讨上皮钙黏附蛋白(E-cad)、β-连接素(β-cat)和基质金属蛋白酶-7(MMP-7)在遗传性非息肉病性大肠癌(HNPCC)和散发性大肠癌(sporadic CRC)的表达及与遗传性非息肉病性大肠癌(HNPCC)侵袭转移间的关系.方法:应用免疫组化SP法检测HNPCC(n= 30)、散发性大肠癌(sporadic CRC)(n=30)和正常大肠黏膜(n=8)中E-cad,β-cat和MMP-7的表达情况.结果:E-cad和胞膜表达β-cat在HNPCC中的阳性表达率高于其在sporadic CRC中的表达率(E-cad:86.7% vs 60.0%:胞膜β-cat:83-3% vs 50.0%,均P<0.05).而胞质表达β-cat和MMP-7则相反(胞质β-cat:46.7% vs 80.0%;MMP-7:46.7% vs 86.7%;均P>0.05).两组中E-cad,胞膜表达β-cat,胞质表达β-cat,MMP-7的阳性表达率与患者的性别和肿瘤的大小、部位及分化类型均无关:而与肿瘤的侵犯深度和转移与否密切相关(r=0.732、P<0.05).E-cad与胞膜表达β-cat呈正相关(r=0.477,P<0.05),胞膜表达β-cat与胞质表达β-cat呈负相关(r=-0.419,P<0.05),而胞质表达β-cat与MMP-7呈正相关(r=0.380,P<0.05).结论:E-cad,β-cat和MMP-7在HNPCC和sporadicc CRC中的阳性表达率差异显著.可能是HNPCC在获得诊断时侵袭弱、转移少和预后较好的原因之一.展开更多
文摘AIM: To study the expression of cyclooxygenase-2 (COX-2)gene in gastric cancer and the relationship between COX-2expression and clinicopathologic features of gastric cancer.METHODS: With reference to the expression of β-actin gene,COX-2 mRNA level was examined in cancerous tissues andadjacent noncancerous mucosa from 33 patients bysemiquantitative reverse transcription- polymerase chainreaction (RT-PCR). Quantitation of relative band Adj volumecounts was performed using molecular Analyst for windowssoftware. The COX-2 index was determined from the band Adjvolume counts ratio of COX-2 to constitutively expressed actin.RESULTS: The COX-2 index in gastric carcinoma wassignificantly higher than that in normal mucosa (0.5966±0.2659vs 0.2979+0.171, u=5.4309, P＜0.01). Significantly higherexpression of COX-2 mRNA was also observed in patientswith lymph node involvement than that in those without(0.6775±0.2486 vs 0.4105±0.2182, t=2.9341, P＜0.01).Furthermore, the staging in the UICC TNM classificationsignificantly correlated with COX-2 overexpression (F＝3.656,P＜0.05), the COX-2 index in stage Ⅲ and IV was significantlyhigher than those in stage Ⅰ and Ⅱ(q=3.2728 and q=3.4906, P＜0.05). The COX-2 index showed no correlationwith patient's age, sex, blood group, tumor location, grosstyping, depth of invasion, differentiation, and the greatesttumor dimension (P＞0.05).CONCLUSION: Expression of COX-2 mRNA in gastriccarcinoma was significantly higher, which may enhancelymphatic metastasis in patients with gastric carcinoma. Thestaging in the UICC TNM classification was significantlycorrelated with COX-2 over-expression. COX-2 may contributeto progression of tumor in human gastric adenocarcinoma.
文摘AIM: To elucidate the expression of E-cadherin and β-catenincorrelating with its clinical outcome in patients withesophageal squamous cell carcinoma (ESCC), by analyzingtheir interrelationship with clinicopathological variables andtheir effects on progress and prognosis.METHODS: Expression of E-cadherin and β-catenin wasdetermined by SP immunohistochemical technique inpatients with ESCC consecutively, their correlation withclinical characteristics was evaluated and analyzed bymultivariate analysis.RESULTS: The rate of expression of E-cadherin decreasedto 66.03 % (70/106) in ESCC and the protein level wasnegative correlated with histologic grade, tumor size, clinicalstaging, lymph node metastasis and venous invasion.Whereas the expression rate of β-catenin was reduced to69.8 % (74/106) and the level of protein expressioncorrelated only with histologic grade. There obviously existedinverse correlation between level of E-cadherin protein andsurvival, especially in .stage I, IIa, IIb (P=0.0033), Patientswith low-expressing tumors for β-catenin and non-expressingtumors for E-cadherin/β-catenin had lower survival periodthan those with normal-expressing ones (P=0.0501 andP=0.0080, respectively). Patients with diminished expressionof E-cadherin as grade Ⅱ or Ⅲ had shorter survival periodthan those with normally expressing and grade Ⅰ, nosignificance existed between grade I and grade Ⅱ or Ⅲwith respect to different status of E-cadherin expression.Furthermore, Correlation analysis showed level of E-cadherincorrelated with that of β-catenin (P=0.005). Cox proportionalhazards model analysis suggested downregulation of E-cadherin was an important factor indicating poor prognosis.CONCLUSION: As a probable independent prognosticfactor, it correlates with overall and disease free survivalperiod, expression of E-cadherin but not β-catenin maypredict prognosis in patients with ESCC.
文摘目的:探讨上皮钙黏附蛋白(E-cad)、β-连接素(β-cat)和基质金属蛋白酶-7(MMP-7)在遗传性非息肉病性大肠癌(HNPCC)和散发性大肠癌(sporadic CRC)的表达及与遗传性非息肉病性大肠癌(HNPCC)侵袭转移间的关系.方法:应用免疫组化SP法检测HNPCC(n= 30)、散发性大肠癌(sporadic CRC)(n=30)和正常大肠黏膜(n=8)中E-cad,β-cat和MMP-7的表达情况.结果:E-cad和胞膜表达β-cat在HNPCC中的阳性表达率高于其在sporadic CRC中的表达率(E-cad:86.7% vs 60.0%:胞膜β-cat:83-3% vs 50.0%,均P<0.05).而胞质表达β-cat和MMP-7则相反(胞质β-cat:46.7% vs 80.0%;MMP-7:46.7% vs 86.7%;均P>0.05).两组中E-cad,胞膜表达β-cat,胞质表达β-cat,MMP-7的阳性表达率与患者的性别和肿瘤的大小、部位及分化类型均无关:而与肿瘤的侵犯深度和转移与否密切相关(r=0.732、P<0.05).E-cad与胞膜表达β-cat呈正相关(r=0.477,P<0.05),胞膜表达β-cat与胞质表达β-cat呈负相关(r=-0.419,P<0.05),而胞质表达β-cat与MMP-7呈正相关(r=0.380,P<0.05).结论:E-cad,β-cat和MMP-7在HNPCC和sporadicc CRC中的阳性表达率差异显著.可能是HNPCC在获得诊断时侵袭弱、转移少和预后较好的原因之一.
