Pestalotiopsis is a taxonomically confused,pathogenic and chemically creative genus requiring a critical reexamination using a multi-gene phylogeny based on ex-type and ex-epitype cultures.In this study 40 isolates of...Pestalotiopsis is a taxonomically confused,pathogenic and chemically creative genus requiring a critical reexamination using a multi-gene phylogeny based on ex-type and ex-epitype cultures.In this study 40 isolates of Pestalotiopsis,comprised of 28 strains collected from living and dead plant material of various host plants from China were studied by means of morphology and analysis of ITS,β–tubulin and tef1 gene sequence data.Based on molecular and morphological data we describe 14 new species(Pestalotiopsis asiatica,P.chinensis,P.chrysea,P.clavata,P.diversiseta,P.ellipsospora,P.inflexa,P.intermedia,P.linearis,P.rosea,P.saprophyta,P.umberspora,P.unicolor and P.verruculosa)and three species are epitypified(P.adusta,P.clavispora and P.foedans).Of the 10 gene regions(ACT,β-tubulin,CAL,GPDH,GS,ITS,LSU,RPB 1,SSU and tef1)utilized to resolve cryptic Pestalotiopsis species,ITS,β–tubulin and tef1 proved to be the better markers.The other gene regions were less useful due to poor success in PCR amplification and/or in their ability to resolve species boundaries.As a single gene tef1 met the requirements for an ideal candidate and functions well for species delimitation due to its better species resolution and PCR success.Althoughβ-tubulin showed fairly good differences among species,a combination of ITS,β-tubulin and tef1 gene data gave the best resolution as compared to single gene analysis.This work provides a backbone tree for 22 ex-type/epitypified species of Pestalotiopsis and can be used in future studies of the genus.展开更多
以0、3.7×103、3.7×104、3.7×105及3.7×106Bq/mL的氚水(HTO)照射原代培养的神经细胞,照后24小时用Leica AF 6000活细胞工作站测细胞迁移距离,Fluo-3-AM荧光探针负载和流式细胞仪测定细胞内游离钙离子浓度,Western b...以0、3.7×103、3.7×104、3.7×105及3.7×106Bq/mL的氚水(HTO)照射原代培养的神经细胞,照后24小时用Leica AF 6000活细胞工作站测细胞迁移距离,Fluo-3-AM荧光探针负载和流式细胞仪测定细胞内游离钙离子浓度,Western blot法分析细胞内β-tubulin和NCAM蛋白变化,以探讨氚β辐射影响神经细胞迁移的机制。结果发现,随着氚水浓度增大,氚照射剂量的增加,神经细胞迁移距离逐渐减小,细胞内游离钙离子逐渐增多,β-tubulin和NCAM表达逐渐减弱。提示氚β辐射可使神经细胞迁移相关调控因子产生变化,影响其迁移。展开更多
基金We thank Thailand Research Fund(grant:BRG5280002)The National Research Council of Thailand(grant for Pestalotiopsis No:55201020008)+4 种基金Mae Fah Luang University(grant for Pestalotiopsis No:55101020004)The National Natural Science Foundation of China(grant:30930005)the Knowledge Innovation Program of the Chinese Academy of Sciences(grant:KSCX2-YW-Z0935)Mushroom Research Foundation,Chiang Mai,ThailandState Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China and the King Saud University for supporting this research.
文摘Pestalotiopsis is a taxonomically confused,pathogenic and chemically creative genus requiring a critical reexamination using a multi-gene phylogeny based on ex-type and ex-epitype cultures.In this study 40 isolates of Pestalotiopsis,comprised of 28 strains collected from living and dead plant material of various host plants from China were studied by means of morphology and analysis of ITS,β–tubulin and tef1 gene sequence data.Based on molecular and morphological data we describe 14 new species(Pestalotiopsis asiatica,P.chinensis,P.chrysea,P.clavata,P.diversiseta,P.ellipsospora,P.inflexa,P.intermedia,P.linearis,P.rosea,P.saprophyta,P.umberspora,P.unicolor and P.verruculosa)and three species are epitypified(P.adusta,P.clavispora and P.foedans).Of the 10 gene regions(ACT,β-tubulin,CAL,GPDH,GS,ITS,LSU,RPB 1,SSU and tef1)utilized to resolve cryptic Pestalotiopsis species,ITS,β–tubulin and tef1 proved to be the better markers.The other gene regions were less useful due to poor success in PCR amplification and/or in their ability to resolve species boundaries.As a single gene tef1 met the requirements for an ideal candidate and functions well for species delimitation due to its better species resolution and PCR success.Althoughβ-tubulin showed fairly good differences among species,a combination of ITS,β-tubulin and tef1 gene data gave the best resolution as compared to single gene analysis.This work provides a backbone tree for 22 ex-type/epitypified species of Pestalotiopsis and can be used in future studies of the genus.
文摘以0、3.7×103、3.7×104、3.7×105及3.7×106Bq/mL的氚水(HTO)照射原代培养的神经细胞,照后24小时用Leica AF 6000活细胞工作站测细胞迁移距离,Fluo-3-AM荧光探针负载和流式细胞仪测定细胞内游离钙离子浓度,Western blot法分析细胞内β-tubulin和NCAM蛋白变化,以探讨氚β辐射影响神经细胞迁移的机制。结果发现,随着氚水浓度增大,氚照射剂量的增加,神经细胞迁移距离逐渐减小,细胞内游离钙离子逐渐增多,β-tubulin和NCAM表达逐渐减弱。提示氚β辐射可使神经细胞迁移相关调控因子产生变化,影响其迁移。
