Sweet sorghum mutants induced by^(12)C(6+)-ion irradiation were planted under different soil salinity conditions to investigate the mechanisms maintaining the transport and spatial distribution of Na^+. The functions ...Sweet sorghum mutants induced by^(12)C(6+)-ion irradiation were planted under different soil salinity conditions to investigate the mechanisms maintaining the transport and spatial distribution of Na^+. The functions of the synergistic responses of NHX, AKT1, and SOS1 related to Na^+ accumulation were investigated in control(KFJT-CK) sorghum and KF1210-3 and KF1210-4 mutants. The results indicated that the NHX, AKT1, and SOS1 proteins in sweet sorghum are mainly involved in the transport, exclusion, and spatial distribution of Na^+,respectively. In addition to physiological parameters, we also measured the expression levels of NHX, AKT1, and SOS1 genes. The experimental results indicated that 150 m M Na Cl induced marked increases in the transcripts of NHX and SOS1 after 8 and 12 h in the KF1210-3,KF1210-4, and KFJT-CK cultivars. In contrast, however, a decrease in AKT1 was observed. On the basis of our results, we propose a model in which cooperation amongNHX, AKT1, and SOS1 facilitates Na^+ homeostasis in sweet sorghum in response to an increase in salt concentration. Accordingly, study of the regulatory mechanisms in sweet sorghum generated by carbon ion irradiation is essential for the selection of salt-tolerant cultivars.展开更多
利用12C^6+重离子束对黄花蒿干种子辐照后,利用发芽率、根长、下胚轴长和株高4个指标研究不同剂量12C^6+离子束辐照对黄花蒿的生物学效应。结果表明,随着辐照剂量的增加,黄花蒿的存活率和根长都呈现逐渐降低的趋势,而下胚轴长先上升后下...利用12C^6+重离子束对黄花蒿干种子辐照后,利用发芽率、根长、下胚轴长和株高4个指标研究不同剂量12C^6+离子束辐照对黄花蒿的生物学效应。结果表明,随着辐照剂量的增加,黄花蒿的存活率和根长都呈现逐渐降低的趋势,而下胚轴长先上升后下降,株高总体呈现下降的趋势,但在60和90 Gy同时存在矮化与增高的植株。利用SRAP(sequence related amplified polymorphism)技术对辐照后M1代的黄花蒿植株进行研究,结果表明对照组和处理组之间的差异体现为特异性条带的变化。综合分析表明,12C^6+的重离子辐照黄花蒿干种子可以产生显著的当代损伤效应,同时引起植物DNA的多态性变化。展开更多
The study is to investigate the feasibility and advantages of heavy ion beams on radiotherapy. The cellular cycle and apoptosis, cell reproductive death and p53 expression evaluated with flow cytometry, clonogenic sur...The study is to investigate the feasibility and advantages of heavy ion beams on radiotherapy. The cellular cycle and apoptosis, cell reproductive death and p53 expression evaluated with flow cytometry, clonogenic survival assays and Western blot analysis were examined in lung carcinoma cells after exposure to 89.63 MeV/u carbon ion and 6 MV X-ray irradiations, respectively. The results showed that the number colonyforming assay of A549 was higher than that of H1299 cells in two radiation groups; A549 cellular cycle was arrested in G2/M in 12 h and the percentage of apoptosis ascended at each time point of carbon ion radiation with doses, the expression of p53 upregulated with doses exposed to X-ray or carbon ion. The cell number in G2/M of H1299 and apoptosis were increasing at all time points with doses in 12C6+ ion irradiation group. The results suggested that the effects of carbon ions or X rays irradiation on lung carcinoma cells were different, 12C6+ ion irradiation could have more effect on upregulating the expression of p53 than X-ray, and the upregulated expression of p53 might produce the cellular cycle G2/M arrested, apoptosis increasing; and p53 gene might affect the lung cancer cells radiosensitivity.展开更多
基金supported by the Science and Technology Service Network Initiative(STS)program of the Chinese Academy of Sciences(CAS)(KFJ-EW-STS-086)the National Natural Science Foundation of China(No.11275171)+1 种基金the CAS‘‘Light of West China’’ Program(Nos.29Y506020 and 29Y406020)the Youth Innovation Promotion Association of CAS(No.2015337)
文摘Sweet sorghum mutants induced by^(12)C(6+)-ion irradiation were planted under different soil salinity conditions to investigate the mechanisms maintaining the transport and spatial distribution of Na^+. The functions of the synergistic responses of NHX, AKT1, and SOS1 related to Na^+ accumulation were investigated in control(KFJT-CK) sorghum and KF1210-3 and KF1210-4 mutants. The results indicated that the NHX, AKT1, and SOS1 proteins in sweet sorghum are mainly involved in the transport, exclusion, and spatial distribution of Na^+,respectively. In addition to physiological parameters, we also measured the expression levels of NHX, AKT1, and SOS1 genes. The experimental results indicated that 150 m M Na Cl induced marked increases in the transcripts of NHX and SOS1 after 8 and 12 h in the KF1210-3,KF1210-4, and KFJT-CK cultivars. In contrast, however, a decrease in AKT1 was observed. On the basis of our results, we propose a model in which cooperation amongNHX, AKT1, and SOS1 facilitates Na^+ homeostasis in sweet sorghum in response to an increase in salt concentration. Accordingly, study of the regulatory mechanisms in sweet sorghum generated by carbon ion irradiation is essential for the selection of salt-tolerant cultivars.
文摘利用12C^6+重离子束对黄花蒿干种子辐照后,利用发芽率、根长、下胚轴长和株高4个指标研究不同剂量12C^6+离子束辐照对黄花蒿的生物学效应。结果表明,随着辐照剂量的增加,黄花蒿的存活率和根长都呈现逐渐降低的趋势,而下胚轴长先上升后下降,株高总体呈现下降的趋势,但在60和90 Gy同时存在矮化与增高的植株。利用SRAP(sequence related amplified polymorphism)技术对辐照后M1代的黄花蒿植株进行研究,结果表明对照组和处理组之间的差异体现为特异性条带的变化。综合分析表明,12C^6+的重离子辐照黄花蒿干种子可以产生显著的当代损伤效应,同时引起植物DNA的多态性变化。
基金Supported by the National Natural Science Foundation of China (10675151 and 10835011)the Key Scientific Technology Research Projects of Gansu Province (0702NKDA045 and 0801NKDA001) Science and technology brainstorm project of Lanzhou (07-2-07)
文摘The study is to investigate the feasibility and advantages of heavy ion beams on radiotherapy. The cellular cycle and apoptosis, cell reproductive death and p53 expression evaluated with flow cytometry, clonogenic survival assays and Western blot analysis were examined in lung carcinoma cells after exposure to 89.63 MeV/u carbon ion and 6 MV X-ray irradiations, respectively. The results showed that the number colonyforming assay of A549 was higher than that of H1299 cells in two radiation groups; A549 cellular cycle was arrested in G2/M in 12 h and the percentage of apoptosis ascended at each time point of carbon ion radiation with doses, the expression of p53 upregulated with doses exposed to X-ray or carbon ion. The cell number in G2/M of H1299 and apoptosis were increasing at all time points with doses in 12C6+ ion irradiation group. The results suggested that the effects of carbon ions or X rays irradiation on lung carcinoma cells were different, 12C6+ ion irradiation could have more effect on upregulating the expression of p53 than X-ray, and the upregulated expression of p53 might produce the cellular cycle G2/M arrested, apoptosis increasing; and p53 gene might affect the lung cancer cells radiosensitivity.
