The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting...The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments,saving people's lives and preventing epidemics.It is important to establish a quick standard diagnostic test for the detection of the infectious disease(COVID-19)to prevent subsequent secondary spread.Polymerase chain reaction(PCR)is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity.Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler opera-tion.A variety of improved or new approaches also have been developed.This review summarizes the currently available detection methods for coronavirus nucleic acid.It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coro-navirus infection.展开更多
Quantitative description of desorption stages of coalbed methane is an important basis to objectively understand the production of coalbed methane well,to diagnose the production state,and to optimize the management o...Quantitative description of desorption stages of coalbed methane is an important basis to objectively understand the production of coalbed methane well,to diagnose the production state,and to optimize the management of draining and collection of coalbed methane.A series of isothermal adsorption experiments were carried out with 12 anthracite samples from 6 coalbed methane wells located in the south of the Qinshui Basin,based on the results of isothermal adsorption experiments,and an analytical model was developed based on the Langmuir sorption theory.With the model,a numerical method that adopts equivalent desorption rate and its curve was established,which can be used to characterize the staged desorption of coalbed methane.According to the experimental and numerical characterizations,three key pressure points determined by the equivalent desorption rate curvature that defines pressure-declining desorption stage,have been proposed and confirmed,namely,start-up pressure,transition pressure and sensitive pressure.By using these three key pressure points,the process of coalbed methane desorption associated with isothermal adsorption experiments can be divided into four stages,i.e.,zero desorption stage,slow desorption stage,transition desorption stage,and sensitive desorption stage.According to analogy analysis,there are differences and similarities between the processes of coalbed methane desorption identified by isothermal adsorption experiments and observed in gas production.Moreover,it has been found that larger Langmuir volume and ratio of Langmuir constants are beneficial to earlier advent of steady production stage,whereas it is also possible that the declining production stage may occur ahead of schedule.展开更多
GH4169 alloy has been widely used in fields such as aviation, aerospace, and petrochemical, because of its excellent combination of mechanical and processing properties. These properties include good high-temperature ...GH4169 alloy has been widely used in fields such as aviation, aerospace, and petrochemical, because of its excellent combination of mechanical and processing properties. These properties include good high-temperature strength, excellent creep and fatigue resistance, and good processing and welding performance. The requirement for high performance, high reliability, and long service life of modem engines has led to the incentive to develop GH4169 alloys with improved performance, such as increased temperature-bearing capacity, improved creep endurance, and better fatigue resistance. Advances during the past thirty years in ba- sic research and industrial technology related to GH4169 alloy were systematically summarized, including advances in alloy modi- fication, melting process optimization, and hot deformation technology.展开更多
In order to meet the requirements of high reliability,long-lifetime and lightweight in a new generation of aerospace,aviation,high-speed train,and energy power equipment,integrated components are urgently needed to re...In order to meet the requirements of high reliability,long-lifetime and lightweight in a new generation of aerospace,aviation,high-speed train,and energy power equipment,integrated components are urgently needed to replace traditional multi-piece,welded components.The applications of integrated components involve in a series of large-size,complex-shaped,highperformance components made of difficult-to-deform materials,which present a huge challenge for forming ultra-large size integrated components.In this paper,the developments and perspectives of several extreme forming technologies are reviewed,including the sheet hydroforming of ultra-large curved components,dieless hydroforming of ellipsoidal shells,radial-axial ring rolling of rings,in situ manufacturing process of flanges,and local isothermal forging of titanium alloy components.The principle and processes for controlling deformation are briefly illustrated.The forming of typical ultra-large size integrated components and industrial applications are introduced,such as the high strength aluminum alloy,3m in diameter,integrated tank dome first formed by using a sheet blank with a thickness the same as the final component,and a 16m diameter,integrated steel ring rolled by using a single billet.The trends for extreme forming of ultra-large size integrated components are discussed with a goal of providing ideas and fundamental guidance for the further development of new forming processes for extreme-size integrated components in the future.展开更多
Al80Ni6 Y8 Co4 Cu2 amorphous ribbons were isothermally annealed and a mixed structure consisting of α-Al particle with a size of less than 15nm and Al3Ni compound with a size of about 30nm was obtained. The crystall...Al80Ni6 Y8 Co4 Cu2 amorphous ribbons were isothermally annealed and a mixed structure consisting of α-Al particle with a size of less than 15nm and Al3Ni compound with a size of about 30nm was obtained. The crystallization kinetics of Al80Ni6 Y8 Co4 Cu2 amorphous alloy shows that the precipitation of α-Al particles is the growth process controlled by diffusion of the solute elements rejected from the growing crystals. By quenching at different cooling rates, a mixed structure consisting of nanoscale α-Al particles and the remaining glass matrix or structure consisting of nanoscale particle (Al phase or Al3Ni compound) with a size of about 100nm was formed. The addition of Co elements and Cu elements to Al-Ni-Y alloy systems increases the glass formation ability of the alloy and the thermal stability of the supercooled liquid region against crystallization, which results from significant difference of atomic size, strong bonding nature among constituent elements and the low diffisivity of the solute elements due to the concentration gradient in the growing front of crystals.展开更多
The microstructural variation in the non dendritic AlSi 7Mg samples remelted in the semi solid state has been investigated. It is proposed that the primary α Al phases are mainly coarsened by connecting the secondary...The microstructural variation in the non dendritic AlSi 7Mg samples remelted in the semi solid state has been investigated. It is proposed that the primary α Al phases are mainly coarsened by connecting the secondary arms or fine primary α Al phases together at the stage of a small quantity of liquid and slowly coarsened through diffusion at the stage of a great quantity of liquid. The dynamical coarsening equation controlled by diffusion is in good agreement with the equation of d 3- d 3 0= kt and the effect of the starting microstructures on the coarsening of primary α Al phases is gradually decreased when the soaking time is long enough.展开更多
Using the similar compositions of the Ti-microalloyed high-strength steels produced by the thin-slab casting process of compact strip production（CSP）,four thermo-mechanical control processes（TMCP）after the simulat...Using the similar compositions of the Ti-microalloyed high-strength steels produced by the thin-slab casting process of compact strip production（CSP）,four thermo-mechanical control processes（TMCP）after the simulated thickslab casting,i.e.the two hot rolling routes and the two cooling processes,were designed,aiming at achieving the same mechanical properties as the thin strip products.The final microstructures after the four TMCP processes were examined by optical microscope（OM）,scanning electron microscope（SEM）and transmission electron microscope（TEM）.The tensile properties and Charpy impact energy were measured correspondingly.Strain-induced TiC precipitation was found in the two-stage rolling route with the finish rolling temperature at low levels,leading to grain refinement due to the pinning effect during austenite recrystallization.Precipitation hardening in ferrite was observed when a period of isothermal holding was applied after hot rolling.It could be concluded that both finish rolling temperature and the subsequent isothermal holding temperature were crucial for the achieved strength level due to the combined effect of grain refinement and precipitation hardening.At the same time,it was found that the isothermal holding led to poor impact toughness because of remarkable precipitation hardening.Therefore,it was suggested that the precipitation kinetics of titanium carbides in both austenite and ferrite should be investigated in future.展开更多
Avoiding the folding defect and improving the die filling capability in the transitional region are desired in isothermal local loading forming of a large-scale Ti-alloy rib-web component（LTRC）. To achieve a high-pr...Avoiding the folding defect and improving the die filling capability in the transitional region are desired in isothermal local loading forming of a large-scale Ti-alloy rib-web component（LTRC）. To achieve a high-precision LTRC, the folding evolution and die filling process in the transitional region were investigated by 3 D finite element simulation and experiment using an equal-thickness billet（ETB）. It is found that the initial volume distribution in the second-loading region can greatly affect the amount of material transferred into the first-loading region during the second-loading step, and thus lead to the folding defect. Besides, an improper initial volume distribution results in non-concurrent die filling in the cavities of ribs after the second-loading step, and then causes die underfilling. To this end, an unequal-thickness billet（UTB） was employed with the initial volume distribution optimized by the response surface method（RSM）. For a certain eigenstructure, the critical value of the percentage of transferred material determined by the ETB was taken as a constraint condition for avoiding the folding defect in the UTB optimization process,and the die underfilling rate was considered as the optimization objective. Then, based on the RSM models of the percentage of transferred material and the die underfilling rate, non-folding parameter combinations and optimum die filling were achieved. Lastly, an optimized UTB was obtained and verified by the simulation and experiment.展开更多
基金financial support from the National Natural Science Foundation of China(Grant 81973281)the Fundamental Research Funds for the Central Universities(2019FZA7017)Leading Talent of“Ten Thousand Plan”-National High-Level Talents SpecialSupport Plan。
文摘The recent pneumonia outbreak caused by a novel coronavirus(SARS-CoV-2)is posing a great threat to global public health.Therefore,rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments,saving people's lives and preventing epidemics.It is important to establish a quick standard diagnostic test for the detection of the infectious disease(COVID-19)to prevent subsequent secondary spread.Polymerase chain reaction(PCR)is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity.Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler opera-tion.A variety of improved or new approaches also have been developed.This review summarizes the currently available detection methods for coronavirus nucleic acid.It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coro-navirus infection.
基金supported by National KeyBasic Research Program of China (Grant No. 2009CB219605)Key Project of National Natural Science Foundation of China (Grant No.40730422)Grand Science and Technology Special Project of China(Grant No. 2011ZX05034-04)
文摘Quantitative description of desorption stages of coalbed methane is an important basis to objectively understand the production of coalbed methane well,to diagnose the production state,and to optimize the management of draining and collection of coalbed methane.A series of isothermal adsorption experiments were carried out with 12 anthracite samples from 6 coalbed methane wells located in the south of the Qinshui Basin,based on the results of isothermal adsorption experiments,and an analytical model was developed based on the Langmuir sorption theory.With the model,a numerical method that adopts equivalent desorption rate and its curve was established,which can be used to characterize the staged desorption of coalbed methane.According to the experimental and numerical characterizations,three key pressure points determined by the equivalent desorption rate curvature that defines pressure-declining desorption stage,have been proposed and confirmed,namely,start-up pressure,transition pressure and sensitive pressure.By using these three key pressure points,the process of coalbed methane desorption associated with isothermal adsorption experiments can be divided into four stages,i.e.,zero desorption stage,slow desorption stage,transition desorption stage,and sensitive desorption stage.According to analogy analysis,there are differences and similarities between the processes of coalbed methane desorption identified by isothermal adsorption experiments and observed in gas production.Moreover,it has been found that larger Langmuir volume and ratio of Langmuir constants are beneficial to earlier advent of steady production stage,whereas it is also possible that the declining production stage may occur ahead of schedule.
文摘GH4169 alloy has been widely used in fields such as aviation, aerospace, and petrochemical, because of its excellent combination of mechanical and processing properties. These properties include good high-temperature strength, excellent creep and fatigue resistance, and good processing and welding performance. The requirement for high performance, high reliability, and long service life of modem engines has led to the incentive to develop GH4169 alloys with improved performance, such as increased temperature-bearing capacity, improved creep endurance, and better fatigue resistance. Advances during the past thirty years in ba- sic research and industrial technology related to GH4169 alloy were systematically summarized, including advances in alloy modi- fication, melting process optimization, and hot deformation technology.
基金This work was funded in part by the National Key Research and Development Program of China(2017YFB0306304)the National Natural Science Foundation of China(51705102,U1637209).The authors wish to express their gratitude for the funding.
文摘In order to meet the requirements of high reliability,long-lifetime and lightweight in a new generation of aerospace,aviation,high-speed train,and energy power equipment,integrated components are urgently needed to replace traditional multi-piece,welded components.The applications of integrated components involve in a series of large-size,complex-shaped,highperformance components made of difficult-to-deform materials,which present a huge challenge for forming ultra-large size integrated components.In this paper,the developments and perspectives of several extreme forming technologies are reviewed,including the sheet hydroforming of ultra-large curved components,dieless hydroforming of ellipsoidal shells,radial-axial ring rolling of rings,in situ manufacturing process of flanges,and local isothermal forging of titanium alloy components.The principle and processes for controlling deformation are briefly illustrated.The forming of typical ultra-large size integrated components and industrial applications are introduced,such as the high strength aluminum alloy,3m in diameter,integrated tank dome first formed by using a sheet blank with a thickness the same as the final component,and a 16m diameter,integrated steel ring rolled by using a single billet.The trends for extreme forming of ultra-large size integrated components are discussed with a goal of providing ideas and fundamental guidance for the further development of new forming processes for extreme-size integrated components in the future.
文摘Al80Ni6 Y8 Co4 Cu2 amorphous ribbons were isothermally annealed and a mixed structure consisting of α-Al particle with a size of less than 15nm and Al3Ni compound with a size of about 30nm was obtained. The crystallization kinetics of Al80Ni6 Y8 Co4 Cu2 amorphous alloy shows that the precipitation of α-Al particles is the growth process controlled by diffusion of the solute elements rejected from the growing crystals. By quenching at different cooling rates, a mixed structure consisting of nanoscale α-Al particles and the remaining glass matrix or structure consisting of nanoscale particle (Al phase or Al3Ni compound) with a size of about 100nm was formed. The addition of Co elements and Cu elements to Al-Ni-Y alloy systems increases the glass formation ability of the alloy and the thermal stability of the supercooled liquid region against crystallization, which results from significant difference of atomic size, strong bonding nature among constituent elements and the low diffisivity of the solute elements due to the concentration gradient in the growing front of crystals.
文摘The microstructural variation in the non dendritic AlSi 7Mg samples remelted in the semi solid state has been investigated. It is proposed that the primary α Al phases are mainly coarsened by connecting the secondary arms or fine primary α Al phases together at the stage of a small quantity of liquid and slowly coarsened through diffusion at the stage of a great quantity of liquid. The dynamical coarsening equation controlled by diffusion is in good agreement with the equation of d 3- d 3 0= kt and the effect of the starting microstructures on the coarsening of primary α Al phases is gradually decreased when the soaking time is long enough.
文摘Using the similar compositions of the Ti-microalloyed high-strength steels produced by the thin-slab casting process of compact strip production（CSP）,four thermo-mechanical control processes（TMCP）after the simulated thickslab casting,i.e.the two hot rolling routes and the two cooling processes,were designed,aiming at achieving the same mechanical properties as the thin strip products.The final microstructures after the four TMCP processes were examined by optical microscope（OM）,scanning electron microscope（SEM）and transmission electron microscope（TEM）.The tensile properties and Charpy impact energy were measured correspondingly.Strain-induced TiC precipitation was found in the two-stage rolling route with the finish rolling temperature at low levels,leading to grain refinement due to the pinning effect during austenite recrystallization.Precipitation hardening in ferrite was observed when a period of isothermal holding was applied after hot rolling.It could be concluded that both finish rolling temperature and the subsequent isothermal holding temperature were crucial for the achieved strength level due to the combined effect of grain refinement and precipitation hardening.At the same time,it was found that the isothermal holding led to poor impact toughness because of remarkable precipitation hardening.Therefore,it was suggested that the precipitation kinetics of titanium carbides in both austenite and ferrite should be investigated in future.
基金supports of the National Natural Science Foundation of China (No. 51575449)Research Fund of the State Key Laboratory of Solidification Processing (NWPU) of China (No. 104-QP2014)+1 种基金the 111 Project (No. B08040)the Fundamental Research Funds for the Central Universities (3102015AX004)
文摘Avoiding the folding defect and improving the die filling capability in the transitional region are desired in isothermal local loading forming of a large-scale Ti-alloy rib-web component（LTRC）. To achieve a high-precision LTRC, the folding evolution and die filling process in the transitional region were investigated by 3 D finite element simulation and experiment using an equal-thickness billet（ETB）. It is found that the initial volume distribution in the second-loading region can greatly affect the amount of material transferred into the first-loading region during the second-loading step, and thus lead to the folding defect. Besides, an improper initial volume distribution results in non-concurrent die filling in the cavities of ribs after the second-loading step, and then causes die underfilling. To this end, an unequal-thickness billet（UTB） was employed with the initial volume distribution optimized by the response surface method（RSM）. For a certain eigenstructure, the critical value of the percentage of transferred material determined by the ETB was taken as a constraint condition for avoiding the folding defect in the UTB optimization process,and the die underfilling rate was considered as the optimization objective. Then, based on the RSM models of the percentage of transferred material and the die underfilling rate, non-folding parameter combinations and optimum die filling were achieved. Lastly, an optimized UTB was obtained and verified by the simulation and experiment.
