目的:基于"脑-肠-菌"轴探讨健脾益气针法对肥胖鼠肠道菌群及重要抗菌免疫分子Toll样受体4(TLR4)的调控机制。方法:复制营养性肥胖小鼠20只,随机均分为模型组与针刺组,另设正常组。针刺组取天枢、关元、后三里及三阴交等穴,施...目的:基于"脑-肠-菌"轴探讨健脾益气针法对肥胖鼠肠道菌群及重要抗菌免疫分子Toll样受体4(TLR4)的调控机制。方法:复制营养性肥胖小鼠20只,随机均分为模型组与针刺组,另设正常组。针刺组取天枢、关元、后三里及三阴交等穴,施以补法,得气后接通电针维持治疗,比较各组小鼠体质量及Lee’s指数差异;ELISA法测定脑与肠组织中炎性因子白细胞介素(IL)-6、IL-10、肿瘤坏死因子-α(TNF-α)的含量及血清中瘦素(LP)、脂联素(ADPN)及游离脂肪酸(FFA)的含量;免疫组化法检测脑-肠中TLR4蛋白分布变化;采用16S r DNA测序技术分析肠道菌群结构丰度变化。结果:模型组体质量、Lee’s指数及血清LP、ADPN及FFA含量显著高于正常组(P<0.01),与模型组比较,针刺组以上指标显著下降(P<0.01);针刺组脑-肠组织中IL-6、IL-10、TNF-α含量显著低于模型组(P<0.05,P<0.01)。针刺组脑及肠组织TLR4蛋白分布密度明显低于模型组(P<0.01);模型组厚壁菌门、绿弯菌门、芽单胞菌门显著升高(P<0.01),拟杆菌门显著降低(P<0.01),针刺组这些异常菌门显著恢复(P<0.05,P<0.01)。结论:应用健脾益气针法治疗肥胖,调节"脑-肠-菌"轴实现肠道菌群再平衡,达到减肥降脂消炎目的。展开更多
ProBiotic-4 is a probiotic preparation composed of Bifidobacterium lactis,Lactobacillus casei,Bifidobacterium bifidum,and Lactobacillus acidophilus.This study aims to investigate the effects of ProBiotic-4 on the micr...ProBiotic-4 is a probiotic preparation composed of Bifidobacterium lactis,Lactobacillus casei,Bifidobacterium bifidum,and Lactobacillus acidophilus.This study aims to investigate the effects of ProBiotic-4 on the microbiota-gut-brain axis and cognitive deficits,and to explore the underlying molecular mechanism using senescence-accelerated mouse prone 8(SAMP8)mice.ProBiotic-4 was orally administered to 9-month-old SAMP8 mice for 12 weeks.We observed that ProBiotic-4 significantly improved the memory deficits,cerebral neuronal and synaptic injuries,glial activation,and microbiota composition in the feces and brains of aged SAMP8 mice.ProBiotic-4 substantially attenuated aging-related disruption of the intestinal barrier and blood-brain barrier,decreased interleukin-6 and tumor necrosis factor-αat both mRNA and protein levels,reduced plasma and cerebral lipopolysaccharide(LPS)concentration,toll-like receptor 4(TLR4)expression,and nuclear factor-κB(NF-κB)nuclear translocation in the brain.In addition,not only did ProBiotic-4 significantly decreased the levels ofγ-H2 AX,8-hydroxydesoxyguanosine,and retinoic-acid-inducible gene-I(RIG-I),it also abrogated RIG-I multimerization in the brain.These findings suggest that targeting gut microbiota with probiotics may have a therapeutic potential for the deficits of the microbiota-gut-brain axis and cognitive function in aging,and that its mechanism is associated with inhibition of both TLR4-and RIG-I-mediated NF-κB signaling pathway and inflammatory responses.展开更多
文摘目的:基于"脑-肠-菌"轴探讨健脾益气针法对肥胖鼠肠道菌群及重要抗菌免疫分子Toll样受体4(TLR4)的调控机制。方法:复制营养性肥胖小鼠20只,随机均分为模型组与针刺组,另设正常组。针刺组取天枢、关元、后三里及三阴交等穴,施以补法,得气后接通电针维持治疗,比较各组小鼠体质量及Lee’s指数差异;ELISA法测定脑与肠组织中炎性因子白细胞介素(IL)-6、IL-10、肿瘤坏死因子-α(TNF-α)的含量及血清中瘦素(LP)、脂联素(ADPN)及游离脂肪酸(FFA)的含量;免疫组化法检测脑-肠中TLR4蛋白分布变化;采用16S r DNA测序技术分析肠道菌群结构丰度变化。结果:模型组体质量、Lee’s指数及血清LP、ADPN及FFA含量显著高于正常组(P<0.01),与模型组比较,针刺组以上指标显著下降(P<0.01);针刺组脑-肠组织中IL-6、IL-10、TNF-α含量显著低于模型组(P<0.05,P<0.01)。针刺组脑及肠组织TLR4蛋白分布密度明显低于模型组(P<0.01);模型组厚壁菌门、绿弯菌门、芽单胞菌门显著升高(P<0.01),拟杆菌门显著降低(P<0.01),针刺组这些异常菌门显著恢复(P<0.05,P<0.01)。结论:应用健脾益气针法治疗肥胖,调节"脑-肠-菌"轴实现肠道菌群再平衡,达到减肥降脂消炎目的。
基金supported by the National Natural Science Foundation of China(81473219 and 81973307)partly by 111 Project of the National Ministry of Education(B18035,China).
文摘ProBiotic-4 is a probiotic preparation composed of Bifidobacterium lactis,Lactobacillus casei,Bifidobacterium bifidum,and Lactobacillus acidophilus.This study aims to investigate the effects of ProBiotic-4 on the microbiota-gut-brain axis and cognitive deficits,and to explore the underlying molecular mechanism using senescence-accelerated mouse prone 8(SAMP8)mice.ProBiotic-4 was orally administered to 9-month-old SAMP8 mice for 12 weeks.We observed that ProBiotic-4 significantly improved the memory deficits,cerebral neuronal and synaptic injuries,glial activation,and microbiota composition in the feces and brains of aged SAMP8 mice.ProBiotic-4 substantially attenuated aging-related disruption of the intestinal barrier and blood-brain barrier,decreased interleukin-6 and tumor necrosis factor-αat both mRNA and protein levels,reduced plasma and cerebral lipopolysaccharide(LPS)concentration,toll-like receptor 4(TLR4)expression,and nuclear factor-κB(NF-κB)nuclear translocation in the brain.In addition,not only did ProBiotic-4 significantly decreased the levels ofγ-H2 AX,8-hydroxydesoxyguanosine,and retinoic-acid-inducible gene-I(RIG-I),it also abrogated RIG-I multimerization in the brain.These findings suggest that targeting gut microbiota with probiotics may have a therapeutic potential for the deficits of the microbiota-gut-brain axis and cognitive function in aging,and that its mechanism is associated with inhibition of both TLR4-and RIG-I-mediated NF-κB signaling pathway and inflammatory responses.
