Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibratin...Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method.The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate.The（x-intercept） of regression equation was the free drug concentration（Cf）.The plasma protein binding rate was calculated by using the following equation: f=（C0-Cf）/C0.Result The binding rate was kept relatively stable in the studied concentration range.Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.展开更多
With the advantages of biosafety and efficiency,increasing attention has been paid to the devices for gene and macromolecular drug delivery based on mechanoporation.The transient pore formation on the cell membrane al...With the advantages of biosafety and efficiency,increasing attention has been paid to the devices for gene and macromolecular drug delivery based on mechanoporation.The transient pore formation on the cell membrane allows cargo transportation when the membrane areal strain is beyond the critical pore value and below the lysis tension threshold.Based on this principle,we propose a method to apply the proper fluid stress on cells moving in a microchannel under the action of zero-net-mass-flux(ZNMF)jets.In this study,an immersed finite element method(IFEM)is adopted to simulate the interaction between the cells and the fluid fields so as to investigate the cell movement and deformation in this mechanoporation system.To evaluate the efficiency of the cargo delivery,a pore integral is defined as the mean pore rate when the cell passes through the jet region.By analyzing the effects of the parameters,including the pressure gradient along the microchannel,the jet amplitude,and the jet frequency,on the pore integrals,a group of optimized parameters for cargo delivery efficiency are obtained.Additionally,the stability and safety of this system are analyzed in detail.These results are helpful in designing the mechanoporation devices and improving their efficiency of drug delivery.展开更多
文摘Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method.The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate.The（x-intercept） of regression equation was the free drug concentration（Cf）.The plasma protein binding rate was calculated by using the following equation: f=（C0-Cf）/C0.Result The binding rate was kept relatively stable in the studied concentration range.Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.
基金supported by the National Natural Science Foundation of China(Nos.11832017 and 12172209)the Key Research Project of Zhejiang Laboratory of China(No.2021PE0AC02)。
文摘With the advantages of biosafety and efficiency,increasing attention has been paid to the devices for gene and macromolecular drug delivery based on mechanoporation.The transient pore formation on the cell membrane allows cargo transportation when the membrane areal strain is beyond the critical pore value and below the lysis tension threshold.Based on this principle,we propose a method to apply the proper fluid stress on cells moving in a microchannel under the action of zero-net-mass-flux(ZNMF)jets.In this study,an immersed finite element method(IFEM)is adopted to simulate the interaction between the cells and the fluid fields so as to investigate the cell movement and deformation in this mechanoporation system.To evaluate the efficiency of the cargo delivery,a pore integral is defined as the mean pore rate when the cell passes through the jet region.By analyzing the effects of the parameters,including the pressure gradient along the microchannel,the jet amplitude,and the jet frequency,on the pore integrals,a group of optimized parameters for cargo delivery efficiency are obtained.Additionally,the stability and safety of this system are analyzed in detail.These results are helpful in designing the mechanoporation devices and improving their efficiency of drug delivery.
