Objective: To observe the effect of Yinian Jiangya Yin (Decoction for lowering blood pressure to prolong life) on patients with early hypertension and its mechanism on the function of vascular endotheliocytes. Methods...Objective: To observe the effect of Yinian Jiangya Yin (Decoction for lowering blood pressure to prolong life) on patients with early hypertension and its mechanism on the function of vascular endotheliocytes. Methods: The 79 patients with early primary hypertension belonging to the TCM syndrome of stagnation of phlegm and blood stasis in meridians and hyperactivity of the liver-yang were randomly divided into a treatment group of 40 patients treated with Yinian Jiangya Yin and a control group of 39 patients treated with Tianma Gouteng Yin (Decoction of Gastrodia and Uncaria). The changes in score of TCM syndrome and in blood pressure before and after treatment were observed in the two groups. The contents of nitrogen monoxide (NO) and endothelin (ET) in serum after treatment were determined. Results: There was a statistical difference (P<0.05) in score of TCM syndrome, effect of lowering blood pressure, and the contents of ET and NO in serum after treatment between the two groups. Conclusion: The effect of Yinian Jiangya Yin on improving TCM syndrome of patients with primary hypertension in early stage and on lowering blood pressure may be related to its regulating the imbalanced condition between ET and NO for restoring the function of endothelium-dependent vasodilation.展开更多
Objective： To observe the effects of Yinian Jiangya Decoction （颐年降压饮, YNJYD） on cytokine secretion in spontaneoulsy hypertensive rats （SHRs） vascular endothelium. Methods： Aortic endothelial cells （ECs） w...Objective： To observe the effects of Yinian Jiangya Decoction （颐年降压饮, YNJYD） on cytokine secretion in spontaneoulsy hypertensive rats （SHRs） vascular endothelium. Methods： Aortic endothelial cells （ECs） were primarily cultured from SHRs; male SD rats were treated with different doses （high, medium, and low doses） of YNJYD, the blood was collected on the 21st day, and then, the serum was separated. ECs were cocultured with the serum for different time courses, and the culture supernatant concentrations of endothelin （ET）-1, nitric oxide （NO）, tissue-type plasminogen activator （t-PA）, and plasminogen activator inhibitor （PAl-1） were determined by ABC-ELISA methods. Results： ET-1, NO, t-PA, and PAl-1 levels in endothelial cell culture supernatant were increased in a time-dependent manner; YNJYD could significantly elevate NO and t-PA expressions in ECs, while ET-1 and PAl-1 expressions were dramatically decreased; these effects of YNJYD were in a concentration-dependent manner. Conclusion： The therapeutic effect of YNJYD on hypertension is attributed to its effect on regulating vessel dilation and blood coagulation, in which ET-1/NO and PAI-1/t-PA are two Pairs of Divotal mediators.展开更多
基金supported by the Macao Government Foundation for Development of Science and Technology, China (No. 105/2005/A)
文摘Objective: To observe the effect of Yinian Jiangya Yin (Decoction for lowering blood pressure to prolong life) on patients with early hypertension and its mechanism on the function of vascular endotheliocytes. Methods: The 79 patients with early primary hypertension belonging to the TCM syndrome of stagnation of phlegm and blood stasis in meridians and hyperactivity of the liver-yang were randomly divided into a treatment group of 40 patients treated with Yinian Jiangya Yin and a control group of 39 patients treated with Tianma Gouteng Yin (Decoction of Gastrodia and Uncaria). The changes in score of TCM syndrome and in blood pressure before and after treatment were observed in the two groups. The contents of nitrogen monoxide (NO) and endothelin (ET) in serum after treatment were determined. Results: There was a statistical difference (P<0.05) in score of TCM syndrome, effect of lowering blood pressure, and the contents of ET and NO in serum after treatment between the two groups. Conclusion: The effect of Yinian Jiangya Yin on improving TCM syndrome of patients with primary hypertension in early stage and on lowering blood pressure may be related to its regulating the imbalanced condition between ET and NO for restoring the function of endothelium-dependent vasodilation.
基金Supported by Science and Technology Development Fund of Macao Special Administrative Region(No.105/2005/A)
文摘Objective： To observe the effects of Yinian Jiangya Decoction （颐年降压饮, YNJYD） on cytokine secretion in spontaneoulsy hypertensive rats （SHRs） vascular endothelium. Methods： Aortic endothelial cells （ECs） were primarily cultured from SHRs; male SD rats were treated with different doses （high, medium, and low doses） of YNJYD, the blood was collected on the 21st day, and then, the serum was separated. ECs were cocultured with the serum for different time courses, and the culture supernatant concentrations of endothelin （ET）-1, nitric oxide （NO）, tissue-type plasminogen activator （t-PA）, and plasminogen activator inhibitor （PAl-1） were determined by ABC-ELISA methods. Results： ET-1, NO, t-PA, and PAl-1 levels in endothelial cell culture supernatant were increased in a time-dependent manner; YNJYD could significantly elevate NO and t-PA expressions in ECs, while ET-1 and PAl-1 expressions were dramatically decreased; these effects of YNJYD were in a concentration-dependent manner. Conclusion： The therapeutic effect of YNJYD on hypertension is attributed to its effect on regulating vessel dilation and blood coagulation, in which ET-1/NO and PAI-1/t-PA are two Pairs of Divotal mediators.
