This review is focused on using computer image analysis as a means of objective and quantitative characterizing optical images of the macroscopic (e.g. microbial colonies) and the microscopic (e.g. single cell) object...This review is focused on using computer image analysis as a means of objective and quantitative characterizing optical images of the macroscopic (e.g. microbial colonies) and the microscopic (e.g. single cell) objects in the microbiological research. This is the way of making many visual inspection assays more objective and less time and labor consuming. Also, it can provide new visually inaccessible information on relation between some optical parameters and various biological features of the microbial cul-tures. Of special interest is application of image analysis in fluorescence microscopy as it opens new ways of using fluorescence based methodology for single microbial cell studies. Examples of using image analysis in the studies of both the macroscopic and the microscopic microbiological objects obtained by various imaging techniques are presented and discussed.展开更多
对经不同条件二次巴氏杀菌的长保质期酸乳的黏度、脱水收缩作用敏感性(susceptibility to syneresis,STS)和感官品质进行测定,将长保质期酸乳分别置于25、37℃条件下恒温贮藏,定期测定产品的霉菌、酵母菌数及菌落总数。结果表明:随着二...对经不同条件二次巴氏杀菌的长保质期酸乳的黏度、脱水收缩作用敏感性(susceptibility to syneresis,STS)和感官品质进行测定,将长保质期酸乳分别置于25、37℃条件下恒温贮藏,定期测定产品的霉菌、酵母菌数及菌落总数。结果表明:随着二次巴氏杀菌温度的升高,产品的黏度逐渐降低、STS逐渐增大;二次巴氏杀菌条件为60℃、30 s产品的喜好度最高,90℃、30 s杀菌产品的喜好度最低;本研究选择的二次巴氏杀菌条件能将残存的霉菌和酵母菌灭活,产品的菌落总数随着二次巴氏杀菌温度的升高而降低,且贮藏温度越高,产品越容易受到污染。综上所述,选择二次巴氏杀菌条件为70℃、30 s效果最佳。展开更多
The recently developed yeast three-hybrid system is a powerful tool for analyzing RNA-protein interactions in vivo. However, large numbers of false positives are frequently met due to bait RNA-independent activation o...The recently developed yeast three-hybrid system is a powerful tool for analyzing RNA-protein interactions in vivo. However, large numbers of false positives are frequently met due to bait RNA-independent activation of the reporter gene in the library screening using this system. In this report, we coupled the colony color assay with the 5FOA (5-fluoroorotic acid) negative selection in the library screening, and found that this coupled method effectively eliminated bait RNA-independent false positives and hence greatly improved library screening efficiency. We used this method successfully in isolation of cDNA of an RNA-binding protein that might play important roles in certain cellular process. This improvement will facilitate the use of the yeast three-hybrid system in analyzing RNA-protein interaction.展开更多
文摘This review is focused on using computer image analysis as a means of objective and quantitative characterizing optical images of the macroscopic (e.g. microbial colonies) and the microscopic (e.g. single cell) objects in the microbiological research. This is the way of making many visual inspection assays more objective and less time and labor consuming. Also, it can provide new visually inaccessible information on relation between some optical parameters and various biological features of the microbial cul-tures. Of special interest is application of image analysis in fluorescence microscopy as it opens new ways of using fluorescence based methodology for single microbial cell studies. Examples of using image analysis in the studies of both the macroscopic and the microscopic microbiological objects obtained by various imaging techniques are presented and discussed.
基金the State "863" High-Tech R&D Project (Grant No. 863-102-11-03-04).
文摘The recently developed yeast three-hybrid system is a powerful tool for analyzing RNA-protein interactions in vivo. However, large numbers of false positives are frequently met due to bait RNA-independent activation of the reporter gene in the library screening using this system. In this report, we coupled the colony color assay with the 5FOA (5-fluoroorotic acid) negative selection in the library screening, and found that this coupled method effectively eliminated bait RNA-independent false positives and hence greatly improved library screening efficiency. We used this method successfully in isolation of cDNA of an RNA-binding protein that might play important roles in certain cellular process. This improvement will facilitate the use of the yeast three-hybrid system in analyzing RNA-protein interaction.
