目的肿瘤免疫干预活性药物的筛选尚无功能评价方法,探索使用SD大鼠和ICR小鼠建立对人源瘤株抗肿瘤免疫的评价方法,考察牛蒡子苷元对机体抗肿瘤免疫增强作用。方法大鼠皮下注射给予牛蒡子苷元,同时用人源肿瘤细胞对大鼠免疫,分离大鼠白...目的肿瘤免疫干预活性药物的筛选尚无功能评价方法,探索使用SD大鼠和ICR小鼠建立对人源瘤株抗肿瘤免疫的评价方法,考察牛蒡子苷元对机体抗肿瘤免疫增强作用。方法大鼠皮下注射给予牛蒡子苷元,同时用人源肿瘤细胞对大鼠免疫,分离大鼠白细胞、血清并与人源肿瘤细胞共培养,检测培养上清液中乳酸脱氢酶(lactate de hydrogenase,LDH)的含量,考察大鼠白细胞与免疫血清对人源瘤株的杀伤力;应用冻融法制备H22细胞裂解物H22TCL。ICR小鼠给药第1、4和7天H22TCL腹腔免疫,第11天小鼠停药并接种H22细胞于腋下,观察肿瘤的发生率,验证牛蒡子苷元抗肿瘤免疫增强作用。结果牛蒡子苷元能够增强SD大鼠白细胞、血清与人源肿瘤细胞体外共培养上清液中LDH含量;牛蒡子苷元能够显著降低ICR小鼠肿瘤的发生率。结论本研究建立使用SD大鼠的免疫血清、白细胞与肿瘤细胞共培养,检测上清中LDH含量筛选抗肿瘤免疫药物的方法,并且牛蒡子苷元降低了小鼠肿瘤的发生率,从而证实了牛蒡子苷元影响了机体的免疫系统,提高动物的抗肿瘤免疫能力,牛蒡子苷元能够提高机体免疫系统对肿瘤细胞的杀伤作用,提高机体抗肿瘤免疫力。展开更多
This paper is concerned with the relation between the parameters of nonwoven filter and removing of white cells. Melt-blown polypropylene nonwovens are used as filtration materials of white cells filter. According to ...This paper is concerned with the relation between the parameters of nonwoven filter and removing of white cells. Melt-blown polypropylene nonwovens are used as filtration materials of white cells filter. According to the combination of different fiber diameter, different product density and different weight, optimized combination of parameters of nonwoven filter with more effective removing of white cells is obtained. The result is that removing of white cells is 96.90 percent; recovery of red cells is 92.27 percent.展开更多
文摘目的肿瘤免疫干预活性药物的筛选尚无功能评价方法,探索使用SD大鼠和ICR小鼠建立对人源瘤株抗肿瘤免疫的评价方法,考察牛蒡子苷元对机体抗肿瘤免疫增强作用。方法大鼠皮下注射给予牛蒡子苷元,同时用人源肿瘤细胞对大鼠免疫,分离大鼠白细胞、血清并与人源肿瘤细胞共培养,检测培养上清液中乳酸脱氢酶(lactate de hydrogenase,LDH)的含量,考察大鼠白细胞与免疫血清对人源瘤株的杀伤力;应用冻融法制备H22细胞裂解物H22TCL。ICR小鼠给药第1、4和7天H22TCL腹腔免疫,第11天小鼠停药并接种H22细胞于腋下,观察肿瘤的发生率,验证牛蒡子苷元抗肿瘤免疫增强作用。结果牛蒡子苷元能够增强SD大鼠白细胞、血清与人源肿瘤细胞体外共培养上清液中LDH含量;牛蒡子苷元能够显著降低ICR小鼠肿瘤的发生率。结论本研究建立使用SD大鼠的免疫血清、白细胞与肿瘤细胞共培养,检测上清中LDH含量筛选抗肿瘤免疫药物的方法,并且牛蒡子苷元降低了小鼠肿瘤的发生率,从而证实了牛蒡子苷元影响了机体的免疫系统,提高动物的抗肿瘤免疫能力,牛蒡子苷元能够提高机体免疫系统对肿瘤细胞的杀伤作用,提高机体抗肿瘤免疫力。
文摘This paper is concerned with the relation between the parameters of nonwoven filter and removing of white cells. Melt-blown polypropylene nonwovens are used as filtration materials of white cells filter. According to the combination of different fiber diameter, different product density and different weight, optimized combination of parameters of nonwoven filter with more effective removing of white cells is obtained. The result is that removing of white cells is 96.90 percent; recovery of red cells is 92.27 percent.
