目的研究血清肝型碱性磷酸酶 (L - AL P)凝集素亲和层析用于原发性肝癌诊断的价值。方法用麦胚凝集素(WGA)分离血清骨型碱性磷酸酶 (B- AL P)和 L - AL P,再将 L - AL P进行蔓陀萝凝集素 (DSA)亲和层析 ,分离不同亲和力组分。结果正常...目的研究血清肝型碱性磷酸酶 (L - AL P)凝集素亲和层析用于原发性肝癌诊断的价值。方法用麦胚凝集素(WGA)分离血清骨型碱性磷酸酶 (B- AL P)和 L - AL P,再将 L - AL P进行蔓陀萝凝集素 (DSA)亲和层析 ,分离不同亲和力组分。结果正常人血清 L - AL P不与 DSA结合 ,在慢性肝炎及肝硬化患者血清中分离到 DSA弱结合组分 ,原发性肝癌患者血清出现了特有的 DSA强结合组分 ,6 4例原发性肝癌血清中阳性率为 92 .19% ,其中 14例原发性小肝癌 (癌结节小于 5 cm )有 78.5 7%的阳性率。结论 L - AL P的 DSA强结合组分为原发性肝癌特异性标志 ,L - AL展开更多
A Triton X-100-4.0G-D （4.0G-D refers to a 4.0-generation dendrimer） was brought forward as a new phosphorescence labeling reagent. Two types of specific affinity adsorption （AA） reactions （direct method and sandw...A Triton X-100-4.0G-D （4.0G-D refers to a 4.0-generation dendrimer） was brought forward as a new phosphorescence labeling reagent. Two types of specific affinity adsorption （AA） reactions （direct method and sandwich method） were carried out between the labeling product of Triton X-100-4：0G-D-Wheat germ agglutinin （WGA） and alkaline phosphatase （ALP）, the product of AA reaction preserved the good characteristics of room temperature phosphorescence （RTP） of 4.0G-D and △Ip of the product was proportional to the content of ALP. According to the fact stated above, a new method for the determination of trace ALP by affinity adsorption solid substrate-room temperature phosphorimetry （AA-SS-RTP） was established on the basis of WGA labeled with the Triton X-100-4.0G-D. The detection limits were 0.20 ag·spot^-1 （corresponding concentration： 5.0×10^-16 g·mL^-1, namely 5.0×10^-18 mol·L^-1） for a direct method and 0.14 ag·spot^-1 （corresponding concentration： 3.5×10^-16 g·mL^-1, namely 3.5×10^-18 mol·L^-1） for a sandwich method, respectively. For their high sensitivity, good repeatability and high accuracy, the direct method and sandwich method have been successfully appfied to determine the content of ALP in human serum, and the results were coincided with the clinical detection results of the enzyme-linked immunosorbent assay method by the Zhangzhou Hospital of Traditional Chinese Medicine. Meanwhile, the mechanism for the determination of trace ALP by AA-SS-RTP was discussed.展开更多
目的制备苦参碱纳米粒及其麦胚凝集素修饰产物。方法复乳化-溶剂挥发法制备苦参碱纳米粒,以聚乳酸-羟基乙酸共聚物与苦参碱比例、转速和聚乙烯醇浓度为影响因素,粒径、电位、包封率和载药量为评价指标,通过星点设计优化工艺。碳二亚胺...目的制备苦参碱纳米粒及其麦胚凝集素修饰产物。方法复乳化-溶剂挥发法制备苦参碱纳米粒,以聚乳酸-羟基乙酸共聚物与苦参碱比例、转速和聚乙烯醇浓度为影响因素,粒径、电位、包封率和载药量为评价指标,通过星点设计优化工艺。碳二亚胺法制备麦胚凝集素修饰苦参碱纳米粒,以碳二亚胺与N-羟基琥珀酰亚胺比例、麦胚凝集素加入量和孵化时间为影响因素,粒径、电位和修饰率为评价指标,通过均匀设计优化工艺。结果苦参碱纳米粒的最佳条件为聚乳酸-羟基乙酸共聚物与苦参碱比例0.594∶1,转速815 r/min,聚乙烯醇浓度为0.46%,平均粒径、电位、包封率和载药量分别为112.04 nm、-15.38 m V、90.05%和27.14%。其麦胚凝集素修饰产物的最佳条件为碳二亚胺与N-羟基琥珀酰亚胺比例2.8∶0.12,麦胚凝集素加入量3 mg,孵化时间14 h,平均粒径、电位和修饰率分别为474.7 nm、-5.2 m V和69.51%。结论该制备工艺可靠,所得苦参碱纳米粒及其麦胚凝集素修饰产物性质稳定。展开更多
An optical biosensor with a stirred cuvette has been used to monitor the interaction of immobilized wheat germ agglutinin （WGA） with two water-soluble cationic porphyrins, The association constants （Ka） of the fre...An optical biosensor with a stirred cuvette has been used to monitor the interaction of immobilized wheat germ agglutinin （WGA） with two water-soluble cationic porphyrins, The association constants （Ka） of the free base porphyrin and its Zn（Ⅱ） complex form were 2. 66 and 27.31 × 10^5 l,/mol at 20 ℃ respectively. The interactions of the free base porphyrin were further investigated at temperatures between 15 ℃ and 37 ℃, The thermodynamics parameters, changes in free energy, enthalpy and entropy, were -31.23, 22.92, 54.15 ld/mol respectively. The heat capacity change was -355.53 J · mol^-1·K^-1 The binding was driven by entropic contribution, and showed strong enthalpy-entropy compensation. It was governed primarily by hydrophobic forces.展开更多
The region between the preexisting and nascentmembranes of a cleaving Rana egg is a dense protrusionregion of nearly 40μm wide at 180°stage.Later,thisregion differentiate,into an upper part,a strip withlong, bra...The region between the preexisting and nascentmembranes of a cleaving Rana egg is a dense protrusionregion of nearly 40μm wide at 180°stage.Later,thisregion differentiate,into an upper part,a strip withlong, branched and randomly distributed protrusionswhich are derived largely from the preexistingmembrane,and a lower part,a band with microvilli.During 4-and 8-cel1 stages,the strip is almost vanishedand microvilli in the band is shortened.The nascentmembrane is smooth at the 180°stage,then a rough areaappears below the smooth region and quickly expands.Wheat germ agglutinin which can bind to preexistingmembrane interrupts the development of the regionbetween the preexisting and nascent membranes.Detergent,Brij,having the property to increase the areaof nascent membrane,does not interrupt the developmentof the region between the preexisting and nascentmembranes.展开更多
文摘目的研究血清肝型碱性磷酸酶 (L - AL P)凝集素亲和层析用于原发性肝癌诊断的价值。方法用麦胚凝集素(WGA)分离血清骨型碱性磷酸酶 (B- AL P)和 L - AL P,再将 L - AL P进行蔓陀萝凝集素 (DSA)亲和层析 ,分离不同亲和力组分。结果正常人血清 L - AL P不与 DSA结合 ,在慢性肝炎及肝硬化患者血清中分离到 DSA弱结合组分 ,原发性肝癌患者血清出现了特有的 DSA强结合组分 ,6 4例原发性肝癌血清中阳性率为 92 .19% ,其中 14例原发性小肝癌 (癌结节小于 5 cm )有 78.5 7%的阳性率。结论 L - AL P的 DSA强结合组分为原发性肝癌特异性标志 ,L - AL
基金Project supported by Fujian Province Natural Science Foundation （Nos. C0510028, D0510027, 2006J0386）.
文摘A Triton X-100-4.0G-D （4.0G-D refers to a 4.0-generation dendrimer） was brought forward as a new phosphorescence labeling reagent. Two types of specific affinity adsorption （AA） reactions （direct method and sandwich method） were carried out between the labeling product of Triton X-100-4：0G-D-Wheat germ agglutinin （WGA） and alkaline phosphatase （ALP）, the product of AA reaction preserved the good characteristics of room temperature phosphorescence （RTP） of 4.0G-D and △Ip of the product was proportional to the content of ALP. According to the fact stated above, a new method for the determination of trace ALP by affinity adsorption solid substrate-room temperature phosphorimetry （AA-SS-RTP） was established on the basis of WGA labeled with the Triton X-100-4.0G-D. The detection limits were 0.20 ag·spot^-1 （corresponding concentration： 5.0×10^-16 g·mL^-1, namely 5.0×10^-18 mol·L^-1） for a direct method and 0.14 ag·spot^-1 （corresponding concentration： 3.5×10^-16 g·mL^-1, namely 3.5×10^-18 mol·L^-1） for a sandwich method, respectively. For their high sensitivity, good repeatability and high accuracy, the direct method and sandwich method have been successfully appfied to determine the content of ALP in human serum, and the results were coincided with the clinical detection results of the enzyme-linked immunosorbent assay method by the Zhangzhou Hospital of Traditional Chinese Medicine. Meanwhile, the mechanism for the determination of trace ALP by AA-SS-RTP was discussed.
文摘目的制备苦参碱纳米粒及其麦胚凝集素修饰产物。方法复乳化-溶剂挥发法制备苦参碱纳米粒,以聚乳酸-羟基乙酸共聚物与苦参碱比例、转速和聚乙烯醇浓度为影响因素,粒径、电位、包封率和载药量为评价指标,通过星点设计优化工艺。碳二亚胺法制备麦胚凝集素修饰苦参碱纳米粒,以碳二亚胺与N-羟基琥珀酰亚胺比例、麦胚凝集素加入量和孵化时间为影响因素,粒径、电位和修饰率为评价指标,通过均匀设计优化工艺。结果苦参碱纳米粒的最佳条件为聚乳酸-羟基乙酸共聚物与苦参碱比例0.594∶1,转速815 r/min,聚乙烯醇浓度为0.46%,平均粒径、电位、包封率和载药量分别为112.04 nm、-15.38 m V、90.05%和27.14%。其麦胚凝集素修饰产物的最佳条件为碳二亚胺与N-羟基琥珀酰亚胺比例2.8∶0.12,麦胚凝集素加入量3 mg,孵化时间14 h,平均粒径、电位和修饰率分别为474.7 nm、-5.2 m V和69.51%。结论该制备工艺可靠,所得苦参碱纳米粒及其麦胚凝集素修饰产物性质稳定。
文摘An optical biosensor with a stirred cuvette has been used to monitor the interaction of immobilized wheat germ agglutinin （WGA） with two water-soluble cationic porphyrins, The association constants （Ka） of the free base porphyrin and its Zn（Ⅱ） complex form were 2. 66 and 27.31 × 10^5 l,/mol at 20 ℃ respectively. The interactions of the free base porphyrin were further investigated at temperatures between 15 ℃ and 37 ℃, The thermodynamics parameters, changes in free energy, enthalpy and entropy, were -31.23, 22.92, 54.15 ld/mol respectively. The heat capacity change was -355.53 J · mol^-1·K^-1 The binding was driven by entropic contribution, and showed strong enthalpy-entropy compensation. It was governed primarily by hydrophobic forces.
文摘The region between the preexisting and nascentmembranes of a cleaving Rana egg is a dense protrusionregion of nearly 40μm wide at 180°stage.Later,thisregion differentiate,into an upper part,a strip withlong, branched and randomly distributed protrusionswhich are derived largely from the preexistingmembrane,and a lower part,a band with microvilli.During 4-and 8-cel1 stages,the strip is almost vanishedand microvilli in the band is shortened.The nascentmembrane is smooth at the 180°stage,then a rough areaappears below the smooth region and quickly expands.Wheat germ agglutinin which can bind to preexistingmembrane interrupts the development of the regionbetween the preexisting and nascent membranes.Detergent,Brij,having the property to increase the areaof nascent membrane,does not interrupt the developmentof the region between the preexisting and nascentmembranes.
