Four new ent-kaurane diterpenoids,namely,3α-tigloyloxypterokaurene L_(3)(1),ent-17-hydroxy-kaura-9(11),15-dien-19-oic acid(2),and wedelobatins A(3)and B(4),together with 11 known ent-kaurane diterpenoids(5-15),were i...Four new ent-kaurane diterpenoids,namely,3α-tigloyloxypterokaurene L_(3)(1),ent-17-hydroxy-kaura-9(11),15-dien-19-oic acid(2),and wedelobatins A(3)and B(4),together with 11 known ent-kaurane diterpenoids(5-15),were isolated from the ethanol extract of Wedelia trilobata.All the structures of 1-15 were elucidated on the basis of spectroscopic studies.展开更多
In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- ...In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.展开更多
Four new ent-kaurane diterpenes with chiral epoxyangelate moieties, (2′R,3′R)-3 a- (2′,3′-epoxyangeloyloxy)-kaur-16-en-19-oic acid (1), (2′S,3′S)-3 a- (2′,3′-epoxyangeloyloxy)-kaur-16-en-19-oic acid (2), (2′...Four new ent-kaurane diterpenes with chiral epoxyangelate moieties, (2′R,3′R)-3 a- (2′,3′-epoxyangeloyloxy)-kaur-16-en-19-oic acid (1), (2′S,3′S)-3 a- (2′,3′-epoxyangeloyloxy)-kaur-16-en-19-oic acid (2), (2′S,3′R)-3 a- (2',3'-epoxyangeloyloxy)-kaur-16-en-19-oic acid (3) and (2′R,3′S)-3α- (2′,3'-epoxyangeloyloxy)-kaur-16-en-19-oic acid (4), along with eight known diterpenes (5-12), were isolated from Wedelia prostrata. The absolute configurations of the new structures were determined by X-ray crystallography,ECD calculations and chemical methods. All compounds were evaluated for their cytotoxicity activities on human HepG-2 cells,with IC_(50) values of 11.72 ±0.22 μmol/L to 54.75±1.12 μmol/L.展开更多
Wedelia （Sphagneticola trilobata （L.） Pruski） has become one of the most dominant invasive plant species in Fiji. However, the soil seed bank of its monospecific stand and its ability to reproduce by seed is relat...Wedelia （Sphagneticola trilobata （L.） Pruski） has become one of the most dominant invasive plant species in Fiji. However, the soil seed bank of its monospecific stand and its ability to reproduce by seed is relatively unknown. A soil seed bank study was undertaken in a monospecific stand ofS. trilobata in Sawani, Natavea and Wainivesi in south-east Viti Levu, Fiji in March 2012. The soil samples were collected from within 1.0 m2 quadrat taken at 10 spots in each of the study areas and spread thinly over a base of Yates Thrive Premium potting mix in seedling trays and placed in a glasshouse at Koronivia Research Station, Fiji. A total of 23, 26 and 33 plant species were found in the soil seed bank in Wainivesi, Sawani and Natavea respectively which may have succumbed to S. trilobata invasiability. There were ca. 3,800 （17%）, 2,100 （11%） and 2,600 （6%） germinable S. trilobata seeds-m-2 in the soil seed bank in Wainivesi, Sawani and Natavea areas respectively. This study has demonstrated that S. trilobata seeds may have a role in the spread of the invasive species in Fiji and movement of soil to S. trilobata free areas should be restricted.展开更多
基金The authors acknowledge the National Basic Research Program of China(973 Program,2009CB522300)the“West Light”program of Chinese Academy of Sciences.
文摘Four new ent-kaurane diterpenoids,namely,3α-tigloyloxypterokaurene L_(3)(1),ent-17-hydroxy-kaura-9(11),15-dien-19-oic acid(2),and wedelobatins A(3)and B(4),together with 11 known ent-kaurane diterpenoids(5-15),were isolated from the ethanol extract of Wedelia trilobata.All the structures of 1-15 were elucidated on the basis of spectroscopic studies.
基金Supported by the National Natural Science Foundation of China（No.31300285）, the Natural Science Foundation of Fujian Province, China（No.2015J01348）, the Specialized Research Fund for the Doctoral Program of Higher Education of China （No.20110073120072） and the Fujian Province Development Guidance（Key） Project, China（No.2014Y0074）.
文摘In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance（NMR） tech- niques and mass spectrometry and identified to be 3a-phenylpropionoyloxy-ent-kaur-16-en-oic acid（l）. The isolated diterpenes were tested for their cytotoxicity activities via 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bro- mide（MTT） assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines（IC50=6.14 and 8.76 μmol/L）.
基金financially supported by the National Natural Science Foundation of China(Nos.81473116,81673319,81673670)Science and Technology Planning Project of Guangdong Province (Nos. 2016A030303011, 2016B030301004)+2 种基金Natural Science Foundation of Guangdong Province(No. 2017A030313732)China Postdoctoral Science Foundation(No. 55350202)Natural Science Foundation of Chongqing(No. cstc2013jcyjA10065)
文摘Four new ent-kaurane diterpenes with chiral epoxyangelate moieties, (2′R,3′R)-3 a- (2′,3′-epoxyangeloyloxy)-kaur-16-en-19-oic acid (1), (2′S,3′S)-3 a- (2′,3′-epoxyangeloyloxy)-kaur-16-en-19-oic acid (2), (2′S,3′R)-3 a- (2',3'-epoxyangeloyloxy)-kaur-16-en-19-oic acid (3) and (2′R,3′S)-3α- (2′,3'-epoxyangeloyloxy)-kaur-16-en-19-oic acid (4), along with eight known diterpenes (5-12), were isolated from Wedelia prostrata. The absolute configurations of the new structures were determined by X-ray crystallography,ECD calculations and chemical methods. All compounds were evaluated for their cytotoxicity activities on human HepG-2 cells,with IC_(50) values of 11.72 ±0.22 μmol/L to 54.75±1.12 μmol/L.
文摘Wedelia （Sphagneticola trilobata （L.） Pruski） has become one of the most dominant invasive plant species in Fiji. However, the soil seed bank of its monospecific stand and its ability to reproduce by seed is relatively unknown. A soil seed bank study was undertaken in a monospecific stand ofS. trilobata in Sawani, Natavea and Wainivesi in south-east Viti Levu, Fiji in March 2012. The soil samples were collected from within 1.0 m2 quadrat taken at 10 spots in each of the study areas and spread thinly over a base of Yates Thrive Premium potting mix in seedling trays and placed in a glasshouse at Koronivia Research Station, Fiji. A total of 23, 26 and 33 plant species were found in the soil seed bank in Wainivesi, Sawani and Natavea respectively which may have succumbed to S. trilobata invasiability. There were ca. 3,800 （17%）, 2,100 （11%） and 2,600 （6%） germinable S. trilobata seeds-m-2 in the soil seed bank in Wainivesi, Sawani and Natavea areas respectively. This study has demonstrated that S. trilobata seeds may have a role in the spread of the invasive species in Fiji and movement of soil to S. trilobata free areas should be restricted.
