目的通过测定子痫前期血清中相关血管炎性蛋白的表达特征以发掘子痫前期潜在预测标志物。方法首先采用病例对照研究募集2017年9月至2018年9月于复旦大学附属妇产科医院就诊的子痫前期孕妇和同期血压正常的对照组孕妇,基于多因子流式检...目的通过测定子痫前期血清中相关血管炎性蛋白的表达特征以发掘子痫前期潜在预测标志物。方法首先采用病例对照研究募集2017年9月至2018年9月于复旦大学附属妇产科医院就诊的子痫前期孕妇和同期血压正常的对照组孕妇,基于多因子流式检测技术测定孕妇血清中12种相关血管炎性蛋白的浓度。通过非参数t检验进行差异性分析以寻找子痫前期相关血管炎性蛋白。然后,从复旦大学附属妇产科医院组织库中获取具有子痫前期高危因素的孕早期孕妇血清标本,并测定子痫前期相关血管炎性因子的血清浓度,通过受试者工作特征(receiver operating characteristic,ROC)曲线分析评估子痫前期相关血管炎症因子的预测价值。结果39例子痫前期和43例对照孕妇血清测定了10个血管炎症蛋白的浓度。结果显示,血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和肌红蛋白在子痫前期孕妇血清中显著升高,而基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)显著降低。在96例具有子痫前期高危因素的孕早期孕妇血清中测定上述分子的浓度并进行ROC曲线分析,结果显示VCAM-1预测孕晚期子痫发生的曲线下面积(the area under the curve,AUC)达到了0.802,而肌红蛋白和MMP-9不能显著预测子痫前期的发生。结论子痫前期患者中部分血管炎性蛋白表达异常,VCAM-1可能是孕早期子痫前期风险预测的生物标志物。展开更多
Aim: To observe the expression changes of Akt and GSK-3β during vascular inflammatory response and oxidative stress induced by high-fat diet in rats. Methods: 20 male Sprague-Dawley rats were separately fed for 18 we...Aim: To observe the expression changes of Akt and GSK-3β during vascular inflammatory response and oxidative stress induced by high-fat diet in rats. Methods: 20 male Sprague-Dawley rats were separately fed for 18 weeks with two types of diets;a normal diet (control group, CON) or high-fat diet hyperlipidmia group, HLP). Then the body weight, lipid parameter, plasma hepatocyte growth factor (HGF), serum superoxide dismutase (SOD) , malondialdehyde (MDA), Tumor necrosis factor-α (TNF-α), a Soluble intercellular adhesion molecule-1 (sICAM-1), Lectin-like oxidized cellulose low density lipoprotein receptor-1 (LOX-1), as well as aortic endothelial p-GSK-3β, GSK-3β, p-Akt, Akt expressions were determined. Results: In comparison with the control group, the model group showed a significant increase in the levels of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol ( LDL-C) and significant decrease in the level of serum high density lipoprotein cholesterol (HDL-C) after high-fat diet for 18 weeks (p < 0.05 or p < 0.01). Meanwhile, a more obvious increase of plasma HGF, LOX-1 and serum MDA, TNF-α, and sICAM-1 levels were observed relative to the control group (p < 0.05 or p < 0.01). Moreover, high-fat diet significantly increased the phosphorylation of Akt and GSK-3β in rat aorta. Conclusion: Short-term high-fat diet could induce vascular endothelium injury by increasing inflammation and oxidative stress. And PI3K/Akt pathway could play an important role in hyperlipidemia-induced vascular endothelium injury.展开更多
Objective：To analyze the clinical features,histopathologic classification and frequencies of various types of recurrent orbital space-occupying lesions.Methods：A retrospective study was carried out in 253 consecutiv...Objective：To analyze the clinical features,histopathologic classification and frequencies of various types of recurrent orbital space-occupying lesions.Methods：A retrospective study was carried out in 253 consecutive patients with recurrent orbital spaceoccupying lesions treated by surgical excision in the Institute of Orbital Diseases,the General Hospital of the Armed Police Force from January 2009 to December 2010.Results：The patients included 123 males and 130 females aged 2 to 78 years（mean,36.2 years）,and the last recurrence interval after operation ranged from 1 month to 40 years（median,4.75 years）.Of all the cases,159（62.8%）,65（25.7%）,20（7.9%）,8（3.2%） and 1（0.4%） had previously experienced once,twice,three,four and six times of surgeries,respectively.Among them,29（11.5%） cases had recurred 3 times or over,and 37（14.6%） cases got recurrence in 10 or more years postoperatively.Most of the patients with local recurrence presented with various clinical manifestations,while 31（12.3%） cases were symptom-free.Two hundred and thirty-one（91.3%） cases underwent surgical removal of the recurrent orbital lesions,and another 22（8.7%） cases had to receive the exenteration of orbit.Categories of these recurrent orbital lesions after operation were as follows：lacrimal gland tumors,65（25.7%） cases;vasogenic diseases,54（21.3%） cases;neurogenic tumors,42（16.6%） cases;secondary tumors,24（9.5%） cases;orbital inflammation,21（8.3%） cases;myogenic tumors,14（5.5%） cases;fibrous and adipose tumors,12（4.7%） cases;lympho-hematopoietic tumors,7（2.8%） cases;bone or cartilage tumors,7（2.8%） cases;orbital cysts,6（2.4%） cases;and indefinitely differentiated tumor,1（0.4%） case.The 10 top histopathologic diagnoses were lacrimal gland pleomorphic adenoma,hemangiolymphangioma,lacrimal gland adenoid cystic carcinoma,meningioma,inflammatory pseudotumor,neurofibroma,sebaceous gland carcinoma,vascular malformation,rhabdomyosarcoma and hemangioma.Co展开更多
文摘目的通过测定子痫前期血清中相关血管炎性蛋白的表达特征以发掘子痫前期潜在预测标志物。方法首先采用病例对照研究募集2017年9月至2018年9月于复旦大学附属妇产科医院就诊的子痫前期孕妇和同期血压正常的对照组孕妇,基于多因子流式检测技术测定孕妇血清中12种相关血管炎性蛋白的浓度。通过非参数t检验进行差异性分析以寻找子痫前期相关血管炎性蛋白。然后,从复旦大学附属妇产科医院组织库中获取具有子痫前期高危因素的孕早期孕妇血清标本,并测定子痫前期相关血管炎性因子的血清浓度,通过受试者工作特征(receiver operating characteristic,ROC)曲线分析评估子痫前期相关血管炎症因子的预测价值。结果39例子痫前期和43例对照孕妇血清测定了10个血管炎症蛋白的浓度。结果显示,血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和肌红蛋白在子痫前期孕妇血清中显著升高,而基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)显著降低。在96例具有子痫前期高危因素的孕早期孕妇血清中测定上述分子的浓度并进行ROC曲线分析,结果显示VCAM-1预测孕晚期子痫发生的曲线下面积(the area under the curve,AUC)达到了0.802,而肌红蛋白和MMP-9不能显著预测子痫前期的发生。结论子痫前期患者中部分血管炎性蛋白表达异常,VCAM-1可能是孕早期子痫前期风险预测的生物标志物。
文摘Aim: To observe the expression changes of Akt and GSK-3β during vascular inflammatory response and oxidative stress induced by high-fat diet in rats. Methods: 20 male Sprague-Dawley rats were separately fed for 18 weeks with two types of diets;a normal diet (control group, CON) or high-fat diet hyperlipidmia group, HLP). Then the body weight, lipid parameter, plasma hepatocyte growth factor (HGF), serum superoxide dismutase (SOD) , malondialdehyde (MDA), Tumor necrosis factor-α (TNF-α), a Soluble intercellular adhesion molecule-1 (sICAM-1), Lectin-like oxidized cellulose low density lipoprotein receptor-1 (LOX-1), as well as aortic endothelial p-GSK-3β, GSK-3β, p-Akt, Akt expressions were determined. Results: In comparison with the control group, the model group showed a significant increase in the levels of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol ( LDL-C) and significant decrease in the level of serum high density lipoprotein cholesterol (HDL-C) after high-fat diet for 18 weeks (p < 0.05 or p < 0.01). Meanwhile, a more obvious increase of plasma HGF, LOX-1 and serum MDA, TNF-α, and sICAM-1 levels were observed relative to the control group (p < 0.05 or p < 0.01). Moreover, high-fat diet significantly increased the phosphorylation of Akt and GSK-3β in rat aorta. Conclusion: Short-term high-fat diet could induce vascular endothelium injury by increasing inflammation and oxidative stress. And PI3K/Akt pathway could play an important role in hyperlipidemia-induced vascular endothelium injury.
文摘Objective：To analyze the clinical features,histopathologic classification and frequencies of various types of recurrent orbital space-occupying lesions.Methods：A retrospective study was carried out in 253 consecutive patients with recurrent orbital spaceoccupying lesions treated by surgical excision in the Institute of Orbital Diseases,the General Hospital of the Armed Police Force from January 2009 to December 2010.Results：The patients included 123 males and 130 females aged 2 to 78 years（mean,36.2 years）,and the last recurrence interval after operation ranged from 1 month to 40 years（median,4.75 years）.Of all the cases,159（62.8%）,65（25.7%）,20（7.9%）,8（3.2%） and 1（0.4%） had previously experienced once,twice,three,four and six times of surgeries,respectively.Among them,29（11.5%） cases had recurred 3 times or over,and 37（14.6%） cases got recurrence in 10 or more years postoperatively.Most of the patients with local recurrence presented with various clinical manifestations,while 31（12.3%） cases were symptom-free.Two hundred and thirty-one（91.3%） cases underwent surgical removal of the recurrent orbital lesions,and another 22（8.7%） cases had to receive the exenteration of orbit.Categories of these recurrent orbital lesions after operation were as follows：lacrimal gland tumors,65（25.7%） cases;vasogenic diseases,54（21.3%） cases;neurogenic tumors,42（16.6%） cases;secondary tumors,24（9.5%） cases;orbital inflammation,21（8.3%） cases;myogenic tumors,14（5.5%） cases;fibrous and adipose tumors,12（4.7%） cases;lympho-hematopoietic tumors,7（2.8%） cases;bone or cartilage tumors,7（2.8%） cases;orbital cysts,6（2.4%） cases;and indefinitely differentiated tumor,1（0.4%） case.The 10 top histopathologic diagnoses were lacrimal gland pleomorphic adenoma,hemangiolymphangioma,lacrimal gland adenoid cystic carcinoma,meningioma,inflammatory pseudotumor,neurofibroma,sebaceous gland carcinoma,vascular malformation,rhabdomyosarcoma and hemangioma.Co
