Background This study aimed to investigate the effect of pcDNA3.1-vascular endothelial growth factor （VEGF）165 vector on vertebral cartilage endplate vascular buds and intervertebral discs. Methods Rabbits were rand...Background This study aimed to investigate the effect of pcDNA3.1-vascular endothelial growth factor （VEGF）165 vector on vertebral cartilage endplate vascular buds and intervertebral discs. Methods Rabbits were randomly assigned to the control and experimental groups with 10 in each. In the experimental group, we anesthetized the rabbits and exposed the front vertebral body. Using the mark of the longitudinal ossature of the front vertebral body of the lumbar vertebrae, we advanced a needle at the central point of the front fourth and fifth lumbar intervertebral discs and injected 20 pl pcDNA3.1-VEGF165. Similarly, in the control group, we injected 20 IJl pcDNA3.1. At 4 and 8 weeks post-injection, we examined the changes of the vertebral cartilage endplate using X-ray radiograph, histology, and scanning electron microscopy. Results The vertebral cartilage endplate calcification and degeneration in the experimental group were less than those in the control group at 8 weeks post-operation. The average number and diameter of vascular buds obviously increased in the experimental group at 4 and 8 weeks post-operation. The number of vascular buds and the diameter in the region of the inner annulus increased when compared to those in the area near the nucleus pulposus. Conclusions The pcDNA3.1-VEGF165 plasmid can increase the average number and diameter of vascular buds and decelerate intervertebral disc degeneration.展开更多
为了研究金黄色葡萄球菌诱导的乳腺炎中乳腺微动脉的变化,在东北细毛绵羊左侧的乳腺注射1 m L金黄色葡萄球菌(109 CFU/m L),而在右侧乳腺注射1 m L生理盐水作为对照。48 h后,屠宰东北细毛绵羊并取其乳腺做成血管铸型标本,对乳腺微动脉...为了研究金黄色葡萄球菌诱导的乳腺炎中乳腺微动脉的变化,在东北细毛绵羊左侧的乳腺注射1 m L金黄色葡萄球菌(109 CFU/m L),而在右侧乳腺注射1 m L生理盐水作为对照。48 h后,屠宰东北细毛绵羊并取其乳腺做成血管铸型标本,对乳腺微动脉进行管径测量和扫描电镜观察。结果显示,东北细毛绵羊左侧乳腺的微动脉内膜表面呈粗糙状和有球状细胞黏附现象,对球状细胞直径进行测量后,推测该细胞为中性粒细胞。结果表明,金黄色葡萄球菌诱导的乳腺炎能够改变乳腺微动脉的结构。展开更多
基金This study was supported by a grant from the National Natural Science Foundation of China，the Anhui Province Education Department Key Fund Project
文摘Background This study aimed to investigate the effect of pcDNA3.1-vascular endothelial growth factor （VEGF）165 vector on vertebral cartilage endplate vascular buds and intervertebral discs. Methods Rabbits were randomly assigned to the control and experimental groups with 10 in each. In the experimental group, we anesthetized the rabbits and exposed the front vertebral body. Using the mark of the longitudinal ossature of the front vertebral body of the lumbar vertebrae, we advanced a needle at the central point of the front fourth and fifth lumbar intervertebral discs and injected 20 pl pcDNA3.1-VEGF165. Similarly, in the control group, we injected 20 IJl pcDNA3.1. At 4 and 8 weeks post-injection, we examined the changes of the vertebral cartilage endplate using X-ray radiograph, histology, and scanning electron microscopy. Results The vertebral cartilage endplate calcification and degeneration in the experimental group were less than those in the control group at 8 weeks post-operation. The average number and diameter of vascular buds obviously increased in the experimental group at 4 and 8 weeks post-operation. The number of vascular buds and the diameter in the region of the inner annulus increased when compared to those in the area near the nucleus pulposus. Conclusions The pcDNA3.1-VEGF165 plasmid can increase the average number and diameter of vascular buds and decelerate intervertebral disc degeneration.
文摘为了研究金黄色葡萄球菌诱导的乳腺炎中乳腺微动脉的变化,在东北细毛绵羊左侧的乳腺注射1 m L金黄色葡萄球菌(109 CFU/m L),而在右侧乳腺注射1 m L生理盐水作为对照。48 h后,屠宰东北细毛绵羊并取其乳腺做成血管铸型标本,对乳腺微动脉进行管径测量和扫描电镜观察。结果显示,东北细毛绵羊左侧乳腺的微动脉内膜表面呈粗糙状和有球状细胞黏附现象,对球状细胞直径进行测量后,推测该细胞为中性粒细胞。结果表明,金黄色葡萄球菌诱导的乳腺炎能够改变乳腺微动脉的结构。
