[Objectives] To analyze the similarities of Boenninghausenia sessilicarpa Levl. and Boenninghausenia albiflora(Hook.) Meiss by the common and variant peak ratio dual indicator sequence method. [Methods] Four different...[Objectives] To analyze the similarities of Boenninghausenia sessilicarpa Levl. and Boenninghausenia albiflora(Hook.) Meiss by the common and variant peak ratio dual indicator sequence method. [Methods] Four different solvents(petroleum ether, chloroform, ethyl acetate and methanol) were used to extract the chemical components of different polar regions of B. sessilicarpa and B. albiflora. UV-visible spectrophotometry(second derivative method) was used to collect the fingerprints of different solvent extracts, and common and variant peak peak ratios were analyzed for the absorption peak data. [Results] The common peak ratio and variant peak ratio data of ground parts of B. albiflora and B. sessilicarpa was S2∶S5(46.2(54.2, 62.5)), compared with the data of other groups, the common peak ratio was the largest, thus the components of ground parts of B. albiflora and B. sessilicarpa were closest and had the largest similarities; the common peak ratio and variant peak ratio data of the components extracted by chloroform from B. albiflora and B. sessilicarpa was Y2∶Y6(54.2(38.5, 46.2)), compared with the data of other groups, the variant peak ratio was the smallest, thus, the chemical components near the chloroform polarity of two kinds of medicinal materials had the largest similarities and smallest differences. [Conclusions] This method is simple and easy to operate, and the ultraviolet fingerprint data of four different polar organic solvent extracts are used for comprehensive analysis, and the results have high specificity and high accuracy. Besides, there are certain similarities and also differences between the chemical components of B. sessilicarpa and B. albiflora. It is expected to provide a new evaluation method for the variety quality of B. sessilicarpa and B. albiflora.展开更多
[Objectives] The infrared fingerprint spectra of Gastrodia elata Blume of different origins and different varieties were studied.[Methods]One-dimensional infrared spectroscopy was used to analyze G. elata of different...[Objectives] The infrared fingerprint spectra of Gastrodia elata Blume of different origins and different varieties were studied.[Methods]One-dimensional infrared spectroscopy was used to analyze G. elata of different origins and different varieties. Based on the infrared spectra of 14 batches of G. elata,the common peak ratio and variant peak ratio were calculated,the double index sequences of common peak ratio and variant peak ratio were established to compare the similarities between the samples of G. elata. [Results] Infrared fingerprint spectra of G. elata of different origins and different varieties were obtained,and a total of 14 common peaks were calibrated. The maximum and minimum common peak ratios among the G. elata samples were 100. 00% and 73. 68%,respectively; and the maximum and minimum variant peak ratios were 21. 43% and 0%,respectively. [Conclusions]The combination of Fourier transform infrared spectroscopy and common peak ratio-variant peak ratio double-index analysis can provide scientific basis for the quality standard research of G. elata.展开更多
基金Supported by State Key Research and Development Program of Ministry of Science and Technology(2018YFC1708000)Scientific and Technological Project at Department and Bureau Level(2018JC028)Fundamental Research Funds for the Central Universities of Southwest Minzu University(2018NQN13)
文摘[Objectives] To analyze the similarities of Boenninghausenia sessilicarpa Levl. and Boenninghausenia albiflora(Hook.) Meiss by the common and variant peak ratio dual indicator sequence method. [Methods] Four different solvents(petroleum ether, chloroform, ethyl acetate and methanol) were used to extract the chemical components of different polar regions of B. sessilicarpa and B. albiflora. UV-visible spectrophotometry(second derivative method) was used to collect the fingerprints of different solvent extracts, and common and variant peak peak ratios were analyzed for the absorption peak data. [Results] The common peak ratio and variant peak ratio data of ground parts of B. albiflora and B. sessilicarpa was S2∶S5(46.2(54.2, 62.5)), compared with the data of other groups, the common peak ratio was the largest, thus the components of ground parts of B. albiflora and B. sessilicarpa were closest and had the largest similarities; the common peak ratio and variant peak ratio data of the components extracted by chloroform from B. albiflora and B. sessilicarpa was Y2∶Y6(54.2(38.5, 46.2)), compared with the data of other groups, the variant peak ratio was the smallest, thus, the chemical components near the chloroform polarity of two kinds of medicinal materials had the largest similarities and smallest differences. [Conclusions] This method is simple and easy to operate, and the ultraviolet fingerprint data of four different polar organic solvent extracts are used for comprehensive analysis, and the results have high specificity and high accuracy. Besides, there are certain similarities and also differences between the chemical components of B. sessilicarpa and B. albiflora. It is expected to provide a new evaluation method for the variety quality of B. sessilicarpa and B. albiflora.
基金Supported by Science and Technology Support Program of Sichuan Province of China(2016NYZ0036,2014SZ0131)
文摘[Objectives] The infrared fingerprint spectra of Gastrodia elata Blume of different origins and different varieties were studied.[Methods]One-dimensional infrared spectroscopy was used to analyze G. elata of different origins and different varieties. Based on the infrared spectra of 14 batches of G. elata,the common peak ratio and variant peak ratio were calculated,the double index sequences of common peak ratio and variant peak ratio were established to compare the similarities between the samples of G. elata. [Results] Infrared fingerprint spectra of G. elata of different origins and different varieties were obtained,and a total of 14 common peaks were calibrated. The maximum and minimum common peak ratios among the G. elata samples were 100. 00% and 73. 68%,respectively; and the maximum and minimum variant peak ratios were 21. 43% and 0%,respectively. [Conclusions]The combination of Fourier transform infrared spectroscopy and common peak ratio-variant peak ratio double-index analysis can provide scientific basis for the quality standard research of G. elata.
