The experiment was conducted to infect one-day old healthy AA chicks with virulent Marek'sdisease virus(MDV).Compared with the uninfected control chicks,it was undertaken to detect theinductive activity of interlu...The experiment was conducted to infect one-day old healthy AA chicks with virulent Marek'sdisease virus(MDV).Compared with the uninfected control chicks,it was undertaken to detect theinductive activity of interlukin-2,expression of IL-2 recepter(IL-2R)and proliferative reaction ofT cell in the thymus and spleen;to determine the number of α-naphthyl esterase positive T cells,acid phosphatase positive T cells and antibody forming cells in the thymus,Bursa Fabricius,spleen,cecal tonsil, Harder gland and mucosal lympoid tissue of bronchus;to check the number of T cell inperipheral blood as well as the dynamic changes of the amount of IgG, IgM and IgA in the serum,tear, trachea washings and intestinal secretions of infected chickens at 5,25,45 days old,respectively.The experimental results reveal that immunoregulation of IL-2 in immune organs ofinfected chickens was disordered;the cellular and humoral immune functions weresignificantly depressed in the central and peripheral immune organs;the local or mucosal immunedefense function were also markedly decreased in respiratory and digestive tracts.展开更多
day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, ...day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, 14, 28, 42, and 56 days after infection with vMDV, respectively, and chemilunescence (CL) assay was adopted to detect the ability of active oxygen radical (AOR) generation of lymphocytes from thymus, spleen and bursa of Fabricious. The results showed: (1) the activity of total SOD (T-SOD) and Mn-SOD of vMDV-infected chicks decreased significantly (P<0.05, P<0.01) compared to control group, whereas that of Cu, Zn-SOD increased slightly (P>0.05); (2) the activity of Se-GSHPx decreased significantly (P<0.05, P<0.01); (3) the content of LPO increased significantly (P<0.05, P<0.01); (4) the value of CL of lymphocytes increased significantly (P<0.05, P<0.01. These showed that vMDV infection resulted in increasing of generation of AOR and decreasing of activity of AOR scavenging enzyme system, leading to deposition of AOR, intensive lipid peroxidation and conversion of cells in chicks. Which gave a further proof that dynamic changes of detected items were consistent with action of vMDV. These proved that AOR resulting from vMDV infection mediated Marek′s disease (MD) and MD oncoma.展开更多
考察枸杞多糖对雏鸡肿瘤坏死因子α和γ干扰素水平的影响。先给1日龄雏鸡腹腔注射马立克强毒株(0.2 m L/只),后注射不同浓度枸杞多糖悬液,最后一次给药后48 h,采集雏鸡脾组织,分离淋巴细胞,分别以生物法和ELISA方法测定肿瘤坏死因子α(T...考察枸杞多糖对雏鸡肿瘤坏死因子α和γ干扰素水平的影响。先给1日龄雏鸡腹腔注射马立克强毒株(0.2 m L/只),后注射不同浓度枸杞多糖悬液,最后一次给药后48 h,采集雏鸡脾组织,分离淋巴细胞,分别以生物法和ELISA方法测定肿瘤坏死因子α(TNF-α)与γ干扰素(IFN-γ)水平。结果表明,一定浓度的枸杞多糖能提高雏鸡脾淋巴细胞中TNF-α和IFN-γ含量,且在一定范围内呈剂量依赖关系。展开更多
文摘The experiment was conducted to infect one-day old healthy AA chicks with virulent Marek'sdisease virus(MDV).Compared with the uninfected control chicks,it was undertaken to detect theinductive activity of interlukin-2,expression of IL-2 recepter(IL-2R)and proliferative reaction ofT cell in the thymus and spleen;to determine the number of α-naphthyl esterase positive T cells,acid phosphatase positive T cells and antibody forming cells in the thymus,Bursa Fabricius,spleen,cecal tonsil, Harder gland and mucosal lympoid tissue of bronchus;to check the number of T cell inperipheral blood as well as the dynamic changes of the amount of IgG, IgM and IgA in the serum,tear, trachea washings and intestinal secretions of infected chickens at 5,25,45 days old,respectively.The experimental results reveal that immunoregulation of IL-2 in immune organs ofinfected chickens was disordered;the cellular and humoral immune functions weresignificantly depressed in the central and peripheral immune organs;the local or mucosal immunedefense function were also markedly decreased in respiratory and digestive tracts.
文摘day-old AA broilers were conducted with violent Marek′s disease virus (vMDV). The activity of SOD, Se-GSHPx, content of LPO in central, peripheral immune organs and main visceral organs of chicks were detected at 7, 14, 28, 42, and 56 days after infection with vMDV, respectively, and chemilunescence (CL) assay was adopted to detect the ability of active oxygen radical (AOR) generation of lymphocytes from thymus, spleen and bursa of Fabricious. The results showed: (1) the activity of total SOD (T-SOD) and Mn-SOD of vMDV-infected chicks decreased significantly (P<0.05, P<0.01) compared to control group, whereas that of Cu, Zn-SOD increased slightly (P>0.05); (2) the activity of Se-GSHPx decreased significantly (P<0.05, P<0.01); (3) the content of LPO increased significantly (P<0.05, P<0.01); (4) the value of CL of lymphocytes increased significantly (P<0.05, P<0.01. These showed that vMDV infection resulted in increasing of generation of AOR and decreasing of activity of AOR scavenging enzyme system, leading to deposition of AOR, intensive lipid peroxidation and conversion of cells in chicks. Which gave a further proof that dynamic changes of detected items were consistent with action of vMDV. These proved that AOR resulting from vMDV infection mediated Marek′s disease (MD) and MD oncoma.
文摘考察枸杞多糖对雏鸡肿瘤坏死因子α和γ干扰素水平的影响。先给1日龄雏鸡腹腔注射马立克强毒株(0.2 m L/只),后注射不同浓度枸杞多糖悬液,最后一次给药后48 h,采集雏鸡脾组织,分离淋巴细胞,分别以生物法和ELISA方法测定肿瘤坏死因子α(TNF-α)与γ干扰素(IFN-γ)水平。结果表明,一定浓度的枸杞多糖能提高雏鸡脾淋巴细胞中TNF-α和IFN-γ含量,且在一定范围内呈剂量依赖关系。
