By nature,biomarker is the measurable change associated with a physiological or pathophysiological process.Unlike blood which has mechanisms to minimize changes and to keep the internal environment homeostatic,urine i...By nature,biomarker is the measurable change associated with a physiological or pathophysiological process.Unlike blood which has mechanisms to minimize changes and to keep the internal environment homeostatic,urine is more likely to reflect changes of the body and is a better biomarker source.Because of its potential in biomarker discovery,urinary proteins should be preserved comprehensively as the duration of the patients’corresponding medical records.Here,we propose a method to adsorb urinary proteins onto a membrane we named Urimem.This simple and inexpensive method requires minimal sample handling,uses no organic solvents,and is environmentally friendly.Urine samples were filtered through the membrane,and urinary proteins were adsorbed onto the membrane.The proteins on the membrane were dried and stored in a vacuum bag,which keeps the protein pattern faithfully preserved.The membrane may even permit storage at room temperature for weeks.Using this simple and inexpensive method,it is possible to begin preserving urine samples from all consenting people.Thus,medical research especially biomarker research can be conducted more economically.Even more objective large-scale prospective studies will be possible.This method has the potential to change the landscape of medical research and medical practice.展开更多
The separation and determination of captopril in human serum and urine samples by means of high performance liquid chromatography(HPLC) with on-line electrogenerated Mn(Ⅲ) chemiluminescence detection was carried out....The separation and determination of captopril in human serum and urine samples by means of high performance liquid chromatography(HPLC) with on-line electrogenerated Mn(Ⅲ) chemiluminescence detection was carried out. The method was based on the direct chemiluminescence reaction between captopril and Mn(Ⅲ), which was on-line electrogenerated by constant current electrolysis. The chromatographic separation was performed on a nucleosil RP-C18(250 mm×4.6mm i.d., 5 μm) column with an isocratic mobile phase consisting of acetonitrile-1% aqueous acetic acid(volume ratio 6∶4) at a flow-rate of 1.2 mL/min. The temperature was 25 ℃. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. Under the optimal conditions, the linear range and detection limit for captopril are 5—800 ng/mL and 0.9 ng/mL, respectively. The relative standard derivation for 10.0 ng/mL captopril is 2.2%(n=11). The average recoveries for captopril in human serum and urine samples ranged from 94.8% to 103.2%, and the relative standard deviations of the quantitative results are below 3.4%. The proposed method had been applied to the determination of captopril in human serum and urine samples.展开更多
基金supported by the National Basic Research Program of China(2012CB517606,2013CB530805)Program of Introducing Talents of Discipline to Universities(B08007)
文摘By nature,biomarker is the measurable change associated with a physiological or pathophysiological process.Unlike blood which has mechanisms to minimize changes and to keep the internal environment homeostatic,urine is more likely to reflect changes of the body and is a better biomarker source.Because of its potential in biomarker discovery,urinary proteins should be preserved comprehensively as the duration of the patients’corresponding medical records.Here,we propose a method to adsorb urinary proteins onto a membrane we named Urimem.This simple and inexpensive method requires minimal sample handling,uses no organic solvents,and is environmentally friendly.Urine samples were filtered through the membrane,and urinary proteins were adsorbed onto the membrane.The proteins on the membrane were dried and stored in a vacuum bag,which keeps the protein pattern faithfully preserved.The membrane may even permit storage at room temperature for weeks.Using this simple and inexpensive method,it is possible to begin preserving urine samples from all consenting people.Thus,medical research especially biomarker research can be conducted more economically.Even more objective large-scale prospective studies will be possible.This method has the potential to change the landscape of medical research and medical practice.
文摘The separation and determination of captopril in human serum and urine samples by means of high performance liquid chromatography(HPLC) with on-line electrogenerated Mn(Ⅲ) chemiluminescence detection was carried out. The method was based on the direct chemiluminescence reaction between captopril and Mn(Ⅲ), which was on-line electrogenerated by constant current electrolysis. The chromatographic separation was performed on a nucleosil RP-C18(250 mm×4.6mm i.d., 5 μm) column with an isocratic mobile phase consisting of acetonitrile-1% aqueous acetic acid(volume ratio 6∶4) at a flow-rate of 1.2 mL/min. The temperature was 25 ℃. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. Under the optimal conditions, the linear range and detection limit for captopril are 5—800 ng/mL and 0.9 ng/mL, respectively. The relative standard derivation for 10.0 ng/mL captopril is 2.2%(n=11). The average recoveries for captopril in human serum and urine samples ranged from 94.8% to 103.2%, and the relative standard deviations of the quantitative results are below 3.4%. The proposed method had been applied to the determination of captopril in human serum and urine samples.
