A concept of unique peptides （CUP） was proposed and implemented to identify whole-cell proteins from tandem mass spectrometry （MS/MS） ion spectra. A unique peptide is defined as a peptide, irrespective of its leng...A concept of unique peptides （CUP） was proposed and implemented to identify whole-cell proteins from tandem mass spectrometry （MS/MS） ion spectra. A unique peptide is defined as a peptide, irrespective of its length, that exists only in one protein of a proteome of interest, despite the fact that this peptide may appear more than once in the same protein. Integrating CUP, a two-step whole-cell protein identification strategy was developed to further increase the confidence of identified proteins. A dataset containing 40,243 MS/MS ion spectra of Saccharomyces cerevisiae and protein identification tools including Mascot and SEQUEST were used to illustrate the proposed concept and strategy. Without implementing CUP, the proteins identified by SEQUEST are 2.26 fold of those identified by Mascot. When CUP was applied, the proteins beating unique peptides identified by SEQUEST are 3.89 fold of those identified by Mascot. By cross-comparing two sets of identified proteins, only 89 common proteins derived from CUP were found. The key discrepancy between identified proteins was resulted from the filtering criteria employed by each protein identification tool. According to the origin of peptides classified by CUP and the commonality of proteins recognized by protein identification tools, all identified proteins were cross-compared, resulting in four groups of proteins possessing different levels of assigned confidence.展开更多
随着U盘、移动硬盘等USB类移动设备在信息存储和交换应用中应用的普及,由于使用和管理不当引发的信息安全问题层出不穷,本文基于动态口令(One Time Password,OTP)加密体制,采用STC51设计了一款“一次一密”安全U盘。该安全U盘包括动态...随着U盘、移动硬盘等USB类移动设备在信息存储和交换应用中应用的普及,由于使用和管理不当引发的信息安全问题层出不穷,本文基于动态口令(One Time Password,OTP)加密体制,采用STC51设计了一款“一次一密”安全U盘。该安全U盘包括动态口令卡、无线遥控器和安全U盘三部分,动态口令卡生成一次性密码,用户通过无线遥控器输入动态密码,安全U盘接收到正确的无线密钥后开启U盘读写权限。通过实验验证,安全U盘能够有效地通过技术手段将安全隐患降低。展开更多
Digitalization has nowadays raised interest in variable applications of farming.Increase of knowledge level,by means of unique identification,automation and control,farmers gain relevant business profit.This research ...Digitalization has nowadays raised interest in variable applications of farming.Increase of knowledge level,by means of unique identification,automation and control,farmers gain relevant business profit.This research is focused on the utilization of passive radio frequency identification(RFID)technology in silage bale application,both manual and automated level.Challenges arise due to silage conservation,varying environmental and seasonal conditions,different identification environments and RFID operation principle.Further maximum communication signal strength is limited by telecommunication standard regulations(e.g.,ETSI).The applicability of RFID technology with different commercial passive transponders is measured manually in a silage bale of 160 cm in diameter,covering 360 degrees around the bale.In addition,automated field tests are conducted in a real environment,where the data collection system is appended to a tractor and RFID reader antenna in a baler.Manual measurements are conducted as identification distance(meters)and transponder population(number of tags),while automated measurements are based on the number of successfully identified silage bales.Based on the manual measurement results,the most suitable tags for the automated field measurements were chosen,and the applicability to silage bale identification was verified.Field tests showed 100%success,with 151/151 uniquely identified silage bales.Achieved results prove that passive RFID operates well enough in silage bale identification,further enabling the development of digitalization of silage bale life cycle.展开更多
文摘A concept of unique peptides （CUP） was proposed and implemented to identify whole-cell proteins from tandem mass spectrometry （MS/MS） ion spectra. A unique peptide is defined as a peptide, irrespective of its length, that exists only in one protein of a proteome of interest, despite the fact that this peptide may appear more than once in the same protein. Integrating CUP, a two-step whole-cell protein identification strategy was developed to further increase the confidence of identified proteins. A dataset containing 40,243 MS/MS ion spectra of Saccharomyces cerevisiae and protein identification tools including Mascot and SEQUEST were used to illustrate the proposed concept and strategy. Without implementing CUP, the proteins identified by SEQUEST are 2.26 fold of those identified by Mascot. When CUP was applied, the proteins beating unique peptides identified by SEQUEST are 3.89 fold of those identified by Mascot. By cross-comparing two sets of identified proteins, only 89 common proteins derived from CUP were found. The key discrepancy between identified proteins was resulted from the filtering criteria employed by each protein identification tool. According to the origin of peptides classified by CUP and the commonality of proteins recognized by protein identification tools, all identified proteins were cross-compared, resulting in four groups of proteins possessing different levels of assigned confidence.
文摘随着U盘、移动硬盘等USB类移动设备在信息存储和交换应用中应用的普及,由于使用和管理不当引发的信息安全问题层出不穷,本文基于动态口令(One Time Password,OTP)加密体制,采用STC51设计了一款“一次一密”安全U盘。该安全U盘包括动态口令卡、无线遥控器和安全U盘三部分,动态口令卡生成一次性密码,用户通过无线遥控器输入动态密码,安全U盘接收到正确的无线密钥后开启U盘读写权限。通过实验验证,安全U盘能够有效地通过技术手段将安全隐患降低。
文摘Digitalization has nowadays raised interest in variable applications of farming.Increase of knowledge level,by means of unique identification,automation and control,farmers gain relevant business profit.This research is focused on the utilization of passive radio frequency identification(RFID)technology in silage bale application,both manual and automated level.Challenges arise due to silage conservation,varying environmental and seasonal conditions,different identification environments and RFID operation principle.Further maximum communication signal strength is limited by telecommunication standard regulations(e.g.,ETSI).The applicability of RFID technology with different commercial passive transponders is measured manually in a silage bale of 160 cm in diameter,covering 360 degrees around the bale.In addition,automated field tests are conducted in a real environment,where the data collection system is appended to a tractor and RFID reader antenna in a baler.Manual measurements are conducted as identification distance(meters)and transponder population(number of tags),while automated measurements are based on the number of successfully identified silage bales.Based on the manual measurement results,the most suitable tags for the automated field measurements were chosen,and the applicability to silage bale identification was verified.Field tests showed 100%success,with 151/151 uniquely identified silage bales.Achieved results prove that passive RFID operates well enough in silage bale identification,further enabling the development of digitalization of silage bale life cycle.
