目的建立应用SYBR Green I实时荧光定量聚合酶链反应（Real—time PCR，RT—PCR）检测parkin基因外显子重排突变的技术平台，应用该技术对常染色体隐性遗传早发型帕金森综合征（autosomal recessive early—onset parkinsonism，AREP）...目的建立应用SYBR Green I实时荧光定量聚合酶链反应（Real—time PCR，RT—PCR）检测parkin基因外显子重排突变的技术平台，应用该技术对常染色体隐性遗传早发型帕金森综合征（autosomal recessive early—onset parkinsonism，AREP）家系进行parkin基因外显子重排突变分析。方法应用SYBR Green I RT—PCR技术对32个中国AREP家系进行parkin基因外显子重排突变分析。结果14个家系先证者存在parkin基因外显子重排突变，其中3个为纯合缺失突变、3个为复杂杂合缺失突变和8个杂合缺失突变，未发现外显子重复突变，突变主要累及第2～4号外显子。结论建立了应用SYBR Green IRT-PCR技术检测parkin基因外显子重排突变的基因检测平台；中国AREP家系的parkin基因外显子重排突变频率为43．8％，与国外报道相似。展开更多
OBJECTIVE: To observe the inhibitory effect of the Chinese herbal compound Guizhu capsule(CGZC)on lung cancer and explore the possible mechanism underlying its actions by evaluating its effects on the expression of ph...OBJECTIVE: To observe the inhibitory effect of the Chinese herbal compound Guizhu capsule(CGZC)on lung cancer and explore the possible mechanism underlying its actions by evaluating its effects on the expression of phosphate and tension homology deleted on chromsome ten(PTEN) and murine double mimute 2(MDM2) in lung cancer model mice.METHODS: A mouse model of transplanted lung cancer was established by subcutaneous inoculation of cancer cells into the axillae of mice. Twenty-four hours later, the mice were weighed and randomly divided into the model control group, cisplatin group(DDP group), and high, moderate and low dosage CGZC groups, with ten mice in each group. The control mice received an equal volume of distilled water. DDP was intraperitoneally injected at 1 mg/kg in the DDP group, once a day for 3days. CGZC diluted with distilled water was admin-istered at 20 g/kg(10 times the clinical adult dosage) in the high-dose group, 10 g/kg(5 times the clinical adult dosage) in the moderate-dose group and 5 g/kg(2.5 times the clinical adult dosage) in the low-dose group once a day for 10 days. On the11 th day, the mice were weighed and killed. The tumor tissues were weighed and the tumor inhibition rate was calculated. PTEN and MDM2 protein expression were detected by immunohistochemical analysis of tumor tissues.RESULTS: The CGZC high-and moderate-dose groups showed a significant inhibitory effect on tumor growth(P < 0.05); there was no significant difference between the high dose group and the DDP group(P > 0.05). The CGZC high-dose group also showed enhanced expression of PTEN protein(P <0.01) and decreased expression of MDM2 protein(P < 0.01) in lung cancer cells of the mice. There was no significant difference between the high dose group and the DDP group.CONCLUSION: CGZC has a significant inhibitory effect on transplanted lung cancer in mice. The mechanism may involve reducing expression of PTEN and decreasing the expression of MDM2 in the lung cancer tissues of the mice.展开更多
文摘目的建立应用SYBR Green I实时荧光定量聚合酶链反应（Real—time PCR，RT—PCR）检测parkin基因外显子重排突变的技术平台，应用该技术对常染色体隐性遗传早发型帕金森综合征（autosomal recessive early—onset parkinsonism，AREP）家系进行parkin基因外显子重排突变分析。方法应用SYBR Green I RT—PCR技术对32个中国AREP家系进行parkin基因外显子重排突变分析。结果14个家系先证者存在parkin基因外显子重排突变，其中3个为纯合缺失突变、3个为复杂杂合缺失突变和8个杂合缺失突变，未发现外显子重复突变，突变主要累及第2～4号外显子。结论建立了应用SYBR Green IRT-PCR技术检测parkin基因外显子重排突变的基因检测平台；中国AREP家系的parkin基因外显子重排突变频率为43．8％，与国外报道相似。
基金Supported by Science and Technology Project of Guangdong Province(No.73062)the Guangzhou Municipal Science and Technology Project(No.2007Z3-E5091)
文摘OBJECTIVE: To observe the inhibitory effect of the Chinese herbal compound Guizhu capsule(CGZC)on lung cancer and explore the possible mechanism underlying its actions by evaluating its effects on the expression of phosphate and tension homology deleted on chromsome ten(PTEN) and murine double mimute 2(MDM2) in lung cancer model mice.METHODS: A mouse model of transplanted lung cancer was established by subcutaneous inoculation of cancer cells into the axillae of mice. Twenty-four hours later, the mice were weighed and randomly divided into the model control group, cisplatin group(DDP group), and high, moderate and low dosage CGZC groups, with ten mice in each group. The control mice received an equal volume of distilled water. DDP was intraperitoneally injected at 1 mg/kg in the DDP group, once a day for 3days. CGZC diluted with distilled water was admin-istered at 20 g/kg(10 times the clinical adult dosage) in the high-dose group, 10 g/kg(5 times the clinical adult dosage) in the moderate-dose group and 5 g/kg(2.5 times the clinical adult dosage) in the low-dose group once a day for 10 days. On the11 th day, the mice were weighed and killed. The tumor tissues were weighed and the tumor inhibition rate was calculated. PTEN and MDM2 protein expression were detected by immunohistochemical analysis of tumor tissues.RESULTS: The CGZC high-and moderate-dose groups showed a significant inhibitory effect on tumor growth(P < 0.05); there was no significant difference between the high dose group and the DDP group(P > 0.05). The CGZC high-dose group also showed enhanced expression of PTEN protein(P <0.01) and decreased expression of MDM2 protein(P < 0.01) in lung cancer cells of the mice. There was no significant difference between the high dose group and the DDP group.CONCLUSION: CGZC has a significant inhibitory effect on transplanted lung cancer in mice. The mechanism may involve reducing expression of PTEN and decreasing the expression of MDM2 in the lung cancer tissues of the mice.
