建立了一种采用KYA H IQ sil C18柱(250 mm×4.6 mm,5μm),以乙腈-水溶液(体积比1∶9)为流动相,在流速1.0 mL/m in、检测波长280 nm、进样量10μL的条件下,用高效液相色谱同时测定红景天苷及其苷元酪醇含量的方法。利用均匀试验设...建立了一种采用KYA H IQ sil C18柱(250 mm×4.6 mm,5μm),以乙腈-水溶液(体积比1∶9)为流动相,在流速1.0 mL/m in、检测波长280 nm、进样量10μL的条件下,用高效液相色谱同时测定红景天苷及其苷元酪醇含量的方法。利用均匀试验设计研究了分析样品的制备方法,确定了红景天根粉的最佳提取条件为:以蒸馏水为溶剂在60℃下超声波提取50 m in。分别测定了不同产地的红景天属植物中红景天苷和酪醇的含量,西藏产圣地红景天中红景天苷含量最高,达到2.147%,其次为藏产大花红景天和西藏红景天,分别为1.763%和1.271%;四川产大花红景天中酪醇含量最高,为0.298 5%,其次是藏产西藏红景天和大花红景天,分别为0.214 3%和0.183 6%;长鞭红景天和狭叶红景天中2种有效成分的含量均很低,红景天苷含量仅为0.034%和0.003%,酪醇含量则分别为0.016 4%和0.004 8%。展开更多
Salidroside is a bioactive tyrosine-derived phenolic natural product found in medicinal plants under the Rhodiola genus. In addition to their anti-fatigue and anti-anoxia roles in traditional medicine, Rhodiola total ...Salidroside is a bioactive tyrosine-derived phenolic natural product found in medicinal plants under the Rhodiola genus. In addition to their anti-fatigue and anti-anoxia roles in traditional medicine, Rhodiola total extract and salidroside have also displayed medicinal properties as anti-cardiovascular diseases and anticancer agents. The resulting surge in global demand of Rhodiola plants and salidroside has driven some species close to extinction. Here, we report the full elucidation of the Rhodiola salidroside biosynthetic pathway utilizing the first comprehensive transcriptomics and metabolomics datasets for Rhodiola rosea. Unlike the previously proposed pathway involving separate decarboxylation and deamination enzymatic steps from tyrosine to the key intermediate 4-hydroxyphenylacetaldehyde （4-HPAA）, Rhodiola contains a pyridoxal phosphate-dependent 4-HPAA synthase that directly converts tyrosine to 4-HPAA. We further identified genes encoding the subsequent 4-HPAA reductase and tyrosohUDP-glucose 8-O-glucosyltransferase, respectively, to complete salidroside biosynthesis in Rhodiola. We show that heterologous production of salidroside can be achieved in the yeast Saccharomyces cerevisiae as well as the plant Nicotiana benthamiana through transgenic expression of Rhodiola salidroside biosynthetic genes. This study provides new tools for engineering sustainable production of salidroside in heterologous hosts.展开更多
文摘建立了一种采用KYA H IQ sil C18柱(250 mm×4.6 mm,5μm),以乙腈-水溶液(体积比1∶9)为流动相,在流速1.0 mL/m in、检测波长280 nm、进样量10μL的条件下,用高效液相色谱同时测定红景天苷及其苷元酪醇含量的方法。利用均匀试验设计研究了分析样品的制备方法,确定了红景天根粉的最佳提取条件为:以蒸馏水为溶剂在60℃下超声波提取50 m in。分别测定了不同产地的红景天属植物中红景天苷和酪醇的含量,西藏产圣地红景天中红景天苷含量最高,达到2.147%,其次为藏产大花红景天和西藏红景天,分别为1.763%和1.271%;四川产大花红景天中酪醇含量最高,为0.298 5%,其次是藏产西藏红景天和大花红景天,分别为0.214 3%和0.183 6%;长鞭红景天和狭叶红景天中2种有效成分的含量均很低,红景天苷含量仅为0.034%和0.003%,酪醇含量则分别为0.016 4%和0.004 8%。
文摘Salidroside is a bioactive tyrosine-derived phenolic natural product found in medicinal plants under the Rhodiola genus. In addition to their anti-fatigue and anti-anoxia roles in traditional medicine, Rhodiola total extract and salidroside have also displayed medicinal properties as anti-cardiovascular diseases and anticancer agents. The resulting surge in global demand of Rhodiola plants and salidroside has driven some species close to extinction. Here, we report the full elucidation of the Rhodiola salidroside biosynthetic pathway utilizing the first comprehensive transcriptomics and metabolomics datasets for Rhodiola rosea. Unlike the previously proposed pathway involving separate decarboxylation and deamination enzymatic steps from tyrosine to the key intermediate 4-hydroxyphenylacetaldehyde （4-HPAA）, Rhodiola contains a pyridoxal phosphate-dependent 4-HPAA synthase that directly converts tyrosine to 4-HPAA. We further identified genes encoding the subsequent 4-HPAA reductase and tyrosohUDP-glucose 8-O-glucosyltransferase, respectively, to complete salidroside biosynthesis in Rhodiola. We show that heterologous production of salidroside can be achieved in the yeast Saccharomyces cerevisiae as well as the plant Nicotiana benthamiana through transgenic expression of Rhodiola salidroside biosynthetic genes. This study provides new tools for engineering sustainable production of salidroside in heterologous hosts.
