Objective: To lay a theoretical foundation for the clinical application of autologous endothelial cell transplantation. Methods: Macaques endothelial cells from the superficial veins were cultivated in vitro for 13. 8...Objective: To lay a theoretical foundation for the clinical application of autologous endothelial cell transplantation. Methods: Macaques endothelial cells from the superficial veins were cultivated in vitro for 13. 89±1. 36d. The multiplied macrophages were lined in vitro onto the luminal surface of expanded polytetrafluoroethylene(ePTFE) grafts precoated with fibrin glue and fibronectin, then cultivated again for 9 d. Fifteen macaque monkeyswere divided randomly into two groups. The common iliac arteries of the experimental animals (n-10) were replaced with endothelialized grafts, those of control (n=5) with untreated ones. Results: The number of endothelialcells increased 147. 93±88. 68 times. All the cells were diploid cells with a purity of 99%. The content of both 6keto-PGFla and vWF in the media showed no significant difference between the primary and subculture passages.Two hours and 9 days after cell seeding, the luminal surface of grafts was covered completely by a spindle-like endothelial monolayer and an even fibrin glue matrix could be seen underneath. Nine days after seeding, the densecondensation of cytoskeleton on the luminal and hasal side of the cells was apparently increased. Four weeks aftergrafts implantation 8/9 of the experimental grafts, with an intima thickness of 80±12 μm, were patent, while the5 control grafts were all occluded. Conclusion: Endothelial cell transplantation could effectively increase the patency rate of synthetic blood vessel prosthesis.展开更多
文摘Objective: To lay a theoretical foundation for the clinical application of autologous endothelial cell transplantation. Methods: Macaques endothelial cells from the superficial veins were cultivated in vitro for 13. 89±1. 36d. The multiplied macrophages were lined in vitro onto the luminal surface of expanded polytetrafluoroethylene(ePTFE) grafts precoated with fibrin glue and fibronectin, then cultivated again for 9 d. Fifteen macaque monkeyswere divided randomly into two groups. The common iliac arteries of the experimental animals (n-10) were replaced with endothelialized grafts, those of control (n=5) with untreated ones. Results: The number of endothelialcells increased 147. 93±88. 68 times. All the cells were diploid cells with a purity of 99%. The content of both 6keto-PGFla and vWF in the media showed no significant difference between the primary and subculture passages.Two hours and 9 days after cell seeding, the luminal surface of grafts was covered completely by a spindle-like endothelial monolayer and an even fibrin glue matrix could be seen underneath. Nine days after seeding, the densecondensation of cytoskeleton on the luminal and hasal side of the cells was apparently increased. Four weeks aftergrafts implantation 8/9 of the experimental grafts, with an intima thickness of 80±12 μm, were patent, while the5 control grafts were all occluded. Conclusion: Endothelial cell transplantation could effectively increase the patency rate of synthetic blood vessel prosthesis.
