The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years(the first,second and third year sample)were analyzed by high-throughput sequencing technology and gas chrom...The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years(the first,second and third year sample)were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography(GC-TOF-MS)to study the effects of microorganisms and small molecular metabolites on the quality of ham in different processing years.The results showed that the dominant bacteria phyla of Nuodeng ham in different processing years were Proteobacteria and Firmicutes,the dominant fungi phyla were Ascomycota and Basidiomycota,while Staphylococcus and Aspergillus were the dominant bacteria and fungi of Nuodeng ham,respectively.Totally,252 kinds of small molecular metabolites were identified from Nuodeng ham in different processing years,and 12 different metabolites were screened through multivariate statistical analysis.Further metabolic pathway analysis showed that 23 metabolic pathways were related to ham fermentation,of which 8 metabolic pathways had significant effects on ham fermentation(Impact>0.01,P<0.05).The content of L-proline,phenyllactic acid,L-lysine,carnosine,taurine,D-proline,betaine and creatine were significantly positively correlated with the relative abundance of Staphylococcus and Serratia,but negatively correlated with the relative abundance of Halomonas,Aspergillus and Yamadazyma.展开更多
本文叙述了盖得的发明专利,从初期的汞扩散泵到分馏油扩散泵的演变过程。讨论了泵体结构、喷咀型式、泵油性能和几个关键技术问题。现代油扩散泵的何氏系数达0.65以上,返油率达10-5 mg/cm2.min,1 kW功率抽气量可达200 Pa L/s。最后对泵...本文叙述了盖得的发明专利,从初期的汞扩散泵到分馏油扩散泵的演变过程。讨论了泵体结构、喷咀型式、泵油性能和几个关键技术问题。现代油扩散泵的何氏系数达0.65以上,返油率达10-5 mg/cm2.min,1 kW功率抽气量可达200 Pa L/s。最后对泵的性能、结构和应用前景作了展望。展开更多
The research is to design a differential pumping system not only to achieve the pressure transition with a large throughput,but also to achieve a clean system without back-oil.In the paper,the pressure in differential...The research is to design a differential pumping system not only to achieve the pressure transition with a large throughput,but also to achieve a clean system without back-oil.In the paper,the pressure in differential stages is calculated;the differential pumping system design and equipment choice are introduced;the tests of Molecular/Booster Pump(MBP),a new kind of molecular-drag pump with large throughout and clean vacuum are described and the system experimental result and analysis are presented.展开更多
Chlorophyll and anthocyanin contents provide a valuable indicator of the status of a plant’s physiology, but to be more widely utilized it needs to be assessed easily and non‐destructively. This is particularly evid...Chlorophyll and anthocyanin contents provide a valuable indicator of the status of a plant’s physiology, but to be more widely utilized it needs to be assessed easily and non‐destructively. This is particularly evident in terms of assessing and exploiting germplasm for plant‐breeding programs. We report, for the first time, experiments with Fragaria chiloensis（L.）Duch. and the estimation of the effects of response to salinity stress（0, 30, and 60 mmol NaCl/L） in terms of these pigments content and gas exchange. It is shown that both pigments（which interestingly, themselves show a high correlation） give a good indication of stress response. Both pigments can be accurately predicted using spectral reflectance indices（SRI）;however, the accuracy of the predictions was slightly improved using multilinear regression analysis models and genetic algorithm analysis. Specifically for chlorophyll content, unlike other species, the use of published SRI gave better indications ofstress response than Normalized Difference Vegetation Index.The effect of salt on gas exchange is only evident at the highest concentration and some SRI gave better prediction performance than the known Photochemical Reflectance Index. This information will therefore be useful for identifying tolerant genotypes to salt stress for incorporation in breeding programs.展开更多
基金supported by Major Science and Technology Projects of Yunnan Science and Technology Plan(2019ZG003)Yunnan Young and Middle-aged Academic and Technical Leader Reserve Talent Project(202105AC160068)。
文摘The internal microbial diversity and small molecular metabolites of Nuodeng ham in different processing years(the first,second and third year sample)were analyzed by high-throughput sequencing technology and gas chromatography-time of flight mass spectrography(GC-TOF-MS)to study the effects of microorganisms and small molecular metabolites on the quality of ham in different processing years.The results showed that the dominant bacteria phyla of Nuodeng ham in different processing years were Proteobacteria and Firmicutes,the dominant fungi phyla were Ascomycota and Basidiomycota,while Staphylococcus and Aspergillus were the dominant bacteria and fungi of Nuodeng ham,respectively.Totally,252 kinds of small molecular metabolites were identified from Nuodeng ham in different processing years,and 12 different metabolites were screened through multivariate statistical analysis.Further metabolic pathway analysis showed that 23 metabolic pathways were related to ham fermentation,of which 8 metabolic pathways had significant effects on ham fermentation(Impact>0.01,P<0.05).The content of L-proline,phenyllactic acid,L-lysine,carnosine,taurine,D-proline,betaine and creatine were significantly positively correlated with the relative abundance of Staphylococcus and Serratia,but negatively correlated with the relative abundance of Halomonas,Aspergillus and Yamadazyma.
文摘The research is to design a differential pumping system not only to achieve the pressure transition with a large throughput,but also to achieve a clean system without back-oil.In the paper,the pressure in differential stages is calculated;the differential pumping system design and equipment choice are introduced;the tests of Molecular/Booster Pump(MBP),a new kind of molecular-drag pump with large throughout and clean vacuum are described and the system experimental result and analysis are presented.
基金supported by the equipment grant(FONDEQUIP‐IQM 130073)from CONICYT‐Chilethe Doctoral research grantthe research program"Adaptation of Agriculture to Climate Change(A2C2),"both from Universidad de Talca‐Chile
文摘Chlorophyll and anthocyanin contents provide a valuable indicator of the status of a plant’s physiology, but to be more widely utilized it needs to be assessed easily and non‐destructively. This is particularly evident in terms of assessing and exploiting germplasm for plant‐breeding programs. We report, for the first time, experiments with Fragaria chiloensis（L.）Duch. and the estimation of the effects of response to salinity stress（0, 30, and 60 mmol NaCl/L） in terms of these pigments content and gas exchange. It is shown that both pigments（which interestingly, themselves show a high correlation） give a good indication of stress response. Both pigments can be accurately predicted using spectral reflectance indices（SRI）;however, the accuracy of the predictions was slightly improved using multilinear regression analysis models and genetic algorithm analysis. Specifically for chlorophyll content, unlike other species, the use of published SRI gave better indications ofstress response than Normalized Difference Vegetation Index.The effect of salt on gas exchange is only evident at the highest concentration and some SRI gave better prediction performance than the known Photochemical Reflectance Index. This information will therefore be useful for identifying tolerant genotypes to salt stress for incorporation in breeding programs.
