为了研究LINC(Linker of nucleoskeleton and cytoskeleton)复合体相关基因KASH(Klarsicht/Anc/Syne-1)和SUN(Sad1/UNC84)在海鞘(Ciona robusta)细胞核位置决定中的角色和作用,本研究鉴定并分别克隆了海鞘LINC复合体中编码2种Cr_KASH蛋...为了研究LINC(Linker of nucleoskeleton and cytoskeleton)复合体相关基因KASH(Klarsicht/Anc/Syne-1)和SUN(Sad1/UNC84)在海鞘(Ciona robusta)细胞核位置决定中的角色和作用,本研究鉴定并分别克隆了海鞘LINC复合体中编码2种Cr_KASH蛋白和2种Cr_SUN蛋白的基因。荧光定量PCR方法分析了上述4个基因在海鞘胚胎各个时期的表达量,发现在胚胎发育的尾芽延伸期,几种基因的表达量均有不同程度的提高。原位杂交方法揭示Cr_KASH1、Cr_SUN1和Cr_SUN2特异表达在海鞘胚胎的脊索组织中,而Cr_KASH2则特异表达在肌肉组织。在NIH/3T3细胞中的亚细胞定位分析证实,上述LINC复合体蛋白与不同的细胞骨架交联。通过破坏细胞骨架微丝和微管蛋白的聚合发现会影响KASH和SUN在细胞中的定位和结构,暗示海鞘LINC复合体成分和细胞质骨架蛋白协同作用调控细胞核的形态和位置。本文研究结果表明,在海鞘不同胚胎发育阶段和不同组织中,细胞核骨架或许通过不同LINC蛋白组分与细胞骨架结合影响细胞核的行为。展开更多
The measurement of positions and sizes of radio sources in observations is important for un- derstanding of the flare evolution. For the first time, solar radio spectral fine structures in an M6.5 flare that occurred ...The measurement of positions and sizes of radio sources in observations is important for un- derstanding of the flare evolution. For the first time, solar radio spectral fine structures in an M6.5 flare that occurred on 2013 April 11 were observed simultaneously by several radio instruments at four different observatories： Chinese Solar Broadband Radio Spectrometer at Huairou （SBRS/Huairou）, Ondrejov Radio Spectrograph in the Czech Republic （ORSC/Ondrejov）, Badary Broadband Microwave Spectropolarimeter （BMS/Irkutsk）, and spectrograph/IZMIRAN （Moscow, Troitsk）. The fine structures included microwave zebra patterns （ZPs）, fast pulsations and fiber bursts. They were observed during the flare brightening lo- cated at the tops of a loop arcade as shown in images taken by the extreme ultraviolet （EUV） telescope onboard NASA＇s satellite Solar Dynamics Observatory （SDO）. The flare occurred at 06：58-07：26 UT in solar active region NOAA 11719 located close to the solar disk center. ZPs appeared near high frequency boundaries of the pulsations, and their spectra observed in Huairou and Ondrejov agreed with each other in terms of details. At the beginning of the flare＇s impulsive phase, a strong narrowband ZP burst occurred with a moderate left-handed circular polarization. Then a series of pulsations and ZPs were observed in almost unpolarized emission. After 07：00 UT a ZP appeared with a moderate right-handed polarization. In the flare decay phase （at about 07：25 UT）, ZPs and fiber bursts become strongly right-hand polarized. BMS/Irkutsk spectral observations indicated that the background emission showed a left-handed circular polarization （similar to SBRS/Huairou spectra around 3 GHz）. However, the fine structure appeared in the right-handed polarization. The dynamics of the polarization was associated with the motion of the flare ex- citer, which was observed in EUV images at 171 A and 131 A by the SDO Atmospheric Imaging Assembly （AIA）. Combining magnetograms observe展开更多
文摘为了研究LINC(Linker of nucleoskeleton and cytoskeleton)复合体相关基因KASH(Klarsicht/Anc/Syne-1)和SUN(Sad1/UNC84)在海鞘(Ciona robusta)细胞核位置决定中的角色和作用,本研究鉴定并分别克隆了海鞘LINC复合体中编码2种Cr_KASH蛋白和2种Cr_SUN蛋白的基因。荧光定量PCR方法分析了上述4个基因在海鞘胚胎各个时期的表达量,发现在胚胎发育的尾芽延伸期,几种基因的表达量均有不同程度的提高。原位杂交方法揭示Cr_KASH1、Cr_SUN1和Cr_SUN2特异表达在海鞘胚胎的脊索组织中,而Cr_KASH2则特异表达在肌肉组织。在NIH/3T3细胞中的亚细胞定位分析证实,上述LINC复合体蛋白与不同的细胞骨架交联。通过破坏细胞骨架微丝和微管蛋白的聚合发现会影响KASH和SUN在细胞中的定位和结构,暗示海鞘LINC复合体成分和细胞质骨架蛋白协同作用调控细胞核的形态和位置。本文研究结果表明,在海鞘不同胚胎发育阶段和不同组织中,细胞核骨架或许通过不同LINC蛋白组分与细胞骨架结合影响细胞核的行为。
基金supported by the Chinese Academy of Sciences Visiting Professorship for Senior International Scientists(Grant No.2011T1J20)funded by Chinese Academy of Sciences President’s International Fellowship Initiative(Grant No.2015VMA014)+3 种基金supported by the Russian Foundation for Basic Research(Grants:13-02-00044,13-02-90472,14-02-91157 and 14-02-00367)the National Natural Science Foundation of China(Grant Nos.11273030,11103044,11103039,11221063,11373039 and 113111042)MOST(Grant2011CB811401)the National Major Scientific Equipment R&D Project(ZDYZ 2009-3 and P209/12/00103 GA CR)
文摘The measurement of positions and sizes of radio sources in observations is important for un- derstanding of the flare evolution. For the first time, solar radio spectral fine structures in an M6.5 flare that occurred on 2013 April 11 were observed simultaneously by several radio instruments at four different observatories： Chinese Solar Broadband Radio Spectrometer at Huairou （SBRS/Huairou）, Ondrejov Radio Spectrograph in the Czech Republic （ORSC/Ondrejov）, Badary Broadband Microwave Spectropolarimeter （BMS/Irkutsk）, and spectrograph/IZMIRAN （Moscow, Troitsk）. The fine structures included microwave zebra patterns （ZPs）, fast pulsations and fiber bursts. They were observed during the flare brightening lo- cated at the tops of a loop arcade as shown in images taken by the extreme ultraviolet （EUV） telescope onboard NASA＇s satellite Solar Dynamics Observatory （SDO）. The flare occurred at 06：58-07：26 UT in solar active region NOAA 11719 located close to the solar disk center. ZPs appeared near high frequency boundaries of the pulsations, and their spectra observed in Huairou and Ondrejov agreed with each other in terms of details. At the beginning of the flare＇s impulsive phase, a strong narrowband ZP burst occurred with a moderate left-handed circular polarization. Then a series of pulsations and ZPs were observed in almost unpolarized emission. After 07：00 UT a ZP appeared with a moderate right-handed polarization. In the flare decay phase （at about 07：25 UT）, ZPs and fiber bursts become strongly right-hand polarized. BMS/Irkutsk spectral observations indicated that the background emission showed a left-handed circular polarization （similar to SBRS/Huairou spectra around 3 GHz）. However, the fine structure appeared in the right-handed polarization. The dynamics of the polarization was associated with the motion of the flare ex- citer, which was observed in EUV images at 171 A and 131 A by the SDO Atmospheric Imaging Assembly （AIA）. Combining magnetograms observe
