Synthetic biology provides a new paradigm for life science research(“build to learn”)and opens the future journey of biotechnology(“build to use”).Here,we discuss advances of various principles and technologies in...Synthetic biology provides a new paradigm for life science research(“build to learn”)and opens the future journey of biotechnology(“build to use”).Here,we discuss advances of various principles and technologies in the mainstream of the enabling technology of synthetic biology,including synthesis and assembly of a genome,DNA storage,gene editing,molecular evolution and de novo design of function proteins,cell and gene circuit engineering,cell-free synthetic biology,artificial intelligence(AI)-aided synthetic biology,as well as biofoundries.We also introduce the concept of quantitative synthetic biology,which is guiding synthetic biology towards increased accuracy and predictability or the real rational design.We conclude that synthetic biology will establish its disciplinary system with the iterative development of enabling technologies and the maturity of the core theory.展开更多
Torque teno virus(TTV) is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3 808 nucleotides of the TTV(SANBAN isolate) genome,which contains a...Torque teno virus(TTV) is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3 808 nucleotides of the TTV(SANBAN isolate) genome,which contains a hairpin structure and a GC-rich region.More than 100 overlapping oligonucleotides were chemically synthesized and assembled by polymerase chain assembly reaction(PCA),and the synthesis was completed with splicing by overlap extension(SOEing).This study establishes the methodological basis of the chemical synthesis of a viral genome for use as a live attenuated vaccine or gene therapy vector.展开更多
The development of preparation methods for XOFs,as a new class of organic frameworks,plays a crucial role in shaping their structures,functions,and potential applications.In this study,we presented the construction of...The development of preparation methods for XOFs,as a new class of organic frameworks,plays a crucial role in shaping their structures,functions,and potential applications.In this study,we presented the construction of chiral supramolecular 2D halogen-bonded organic frameworks(XOFs)through a post-synthetic modification strategy.A linear halogen-bonded organic polymer(XOP),XOP-DPBA,decorated with aldehyde groups,was initially prepared to validate the feasibility of post-synthetic modification for XOF construction and functionalization.XOP-DPBA exhibited excellent reactivity with amines,forming imine bonds.By utilizing this reactivity,a series of cross-linked 2D XOFs were efficiently prepared through post-synthetic modified cross-linking reactions.Furthermore,we successfully introduced chiralα/β-cyclodextrins(α/β-CD)into the XOF skeletons via host-guest interactions,resulting in the fabrication of chiral supramolecular 2D XOFs.These chiral XOFs displayed the induced circular dichroism(ICD)signals and assembled them into helical fibers.The post-synthetic modification strategy demonstrated its versatility and simplicity for the construction and functionalization of XOFs.展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29050100,XDB29050500,XDA24020102)to X.E.Zhang,C.Liu and C.Gao,respectivelythe National Natural Science Foundation of China(31725002,31861143017,32022044,62050152 and 32071428)to J.Dai,Y.Yuan,C.You,and X.Wang,respectivelythe National Key Research and Development Program of China(2020YFA0907700,2018YFA0901600,2019YFA09004500)to Y.Feng and P.Wei。
文摘Synthetic biology provides a new paradigm for life science research(“build to learn”)and opens the future journey of biotechnology(“build to use”).Here,we discuss advances of various principles and technologies in the mainstream of the enabling technology of synthetic biology,including synthesis and assembly of a genome,DNA storage,gene editing,molecular evolution and de novo design of function proteins,cell and gene circuit engineering,cell-free synthetic biology,artificial intelligence(AI)-aided synthetic biology,as well as biofoundries.We also introduce the concept of quantitative synthetic biology,which is guiding synthetic biology towards increased accuracy and predictability or the real rational design.We conclude that synthetic biology will establish its disciplinary system with the iterative development of enabling technologies and the maturity of the core theory.
基金The Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-Z-3)
文摘Torque teno virus(TTV) is a nonenveloped virus containing a single-stranded,circular DNA genome of approximately 3.8kb.We completely synthesized the 3 808 nucleotides of the TTV(SANBAN isolate) genome,which contains a hairpin structure and a GC-rich region.More than 100 overlapping oligonucleotides were chemically synthesized and assembled by polymerase chain assembly reaction(PCA),and the synthesis was completed with splicing by overlap extension(SOEing).This study establishes the methodological basis of the chemical synthesis of a viral genome for use as a live attenuated vaccine or gene therapy vector.
基金supported by the National Natural Science Foundation of China(22371218,21702153,21801194)the Wuhan Science and Technology Bureau(whkxjsj009)+1 种基金the support of the Core Facility of Wuhan Universitythe Large-scale Instrument and Equipment Sharing Foundation of Wuhan University。
文摘The development of preparation methods for XOFs,as a new class of organic frameworks,plays a crucial role in shaping their structures,functions,and potential applications.In this study,we presented the construction of chiral supramolecular 2D halogen-bonded organic frameworks(XOFs)through a post-synthetic modification strategy.A linear halogen-bonded organic polymer(XOP),XOP-DPBA,decorated with aldehyde groups,was initially prepared to validate the feasibility of post-synthetic modification for XOF construction and functionalization.XOP-DPBA exhibited excellent reactivity with amines,forming imine bonds.By utilizing this reactivity,a series of cross-linked 2D XOFs were efficiently prepared through post-synthetic modified cross-linking reactions.Furthermore,we successfully introduced chiralα/β-cyclodextrins(α/β-CD)into the XOF skeletons via host-guest interactions,resulting in the fabrication of chiral supramolecular 2D XOFs.These chiral XOFs displayed the induced circular dichroism(ICD)signals and assembled them into helical fibers.The post-synthetic modification strategy demonstrated its versatility and simplicity for the construction and functionalization of XOFs.