This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference...This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference in the percentage of nucleated fibroblasts in the G0/G1 stage between serum concentrations of 0.3% and 0.4% (83.89% and 82.69%, respectively, P > 0.05) as well as between serum concentrations of 0.2% and 0.5% (76.95% and 75.46%, respectively, P > 0.05). The percentage of nucleated fibroblasts in the G0/G1 stage was highest at the concentration of 0.3% and lowest in the control group (83.89% vs. 62.67%, P 0.05). The beneficial effect of high confluence was confirmed by the large percentage of nucleated fibroblasts at the G0/G1 stage. The 60% confluency was significantly lower than the 80% and 100% confluency (73.44%, 86.63%, and 87.17%, respectively, P < 0.05). The results indicate that the goat fibroblast cycle synchronization is the most effective at the serum concentration of 0.3%, 72 hours of synchronization and 100% confluency.展开更多
Although it is known that in most angiosperms mitosis in early endosperm development is syncytial and synchronized, it is unclear how the synchronization is regulated. We showed previously that APC11, also named ZYGI,...Although it is known that in most angiosperms mitosis in early endosperm development is syncytial and synchronized, it is unclear how the synchronization is regulated. We showed previously that APC11, also named ZYGI, in Arabidopsis activates zygote division by interaction and degradation of cyclin B1. Here, we report that the mutation in APC11/ZYG1 led to unsynchronized mitosis and over-accumulation of cyclin B1-GUS in the endosperm. Mutations in two other APC subunits showed similar defects. Transgenic expression of stable cyclin B1 in the endosperm also caused unsynchronized mitosis. Further, downregulation of APC11 generated multi-nucleate somatic cells with unsynchronized mitotic division. Together, our results suggest that APC/C-mediated cyclin B1 degradation is critical for cell cycle synchronization.展开更多
同步作为小区搜索的重要部分,对小区的选择和响应时延等有着决定性的作用。在长期演进(Long Term Evolution,LTE)系统中主同步直接影响辅同步、全双工模式的识别,以至整个通信系统。结合主同步信号(Primary Synchronization Signal,PSS...同步作为小区搜索的重要部分,对小区的选择和响应时延等有着决定性的作用。在长期演进(Long Term Evolution,LTE)系统中主同步直接影响辅同步、全双工模式的识别,以至整个通信系统。结合主同步信号(Primary Synchronization Signal,PSS)良好的互相关性,给出了一种基于FFT快速相关的改进算法,采用重叠和分段联合检测的方式,并且通过数据循环后移保证序列完整性,来实现对半帧数据的快速搜索。理论分析和仿真表明,该算法能够有效降低计算量和同步时间。实验表明,该算法在LTE系统中能够准确高效地实现PSS序列的快速同步。展开更多
文摘This study aims to evaluate the effect of serum concentration, synchronization time, and confluence degree on the synchronisation efficiency of goat fibroblast cycle. The results indicated that there was no difference in the percentage of nucleated fibroblasts in the G0/G1 stage between serum concentrations of 0.3% and 0.4% (83.89% and 82.69%, respectively, P > 0.05) as well as between serum concentrations of 0.2% and 0.5% (76.95% and 75.46%, respectively, P > 0.05). The percentage of nucleated fibroblasts in the G0/G1 stage was highest at the concentration of 0.3% and lowest in the control group (83.89% vs. 62.67%, P 0.05). The beneficial effect of high confluence was confirmed by the large percentage of nucleated fibroblasts at the G0/G1 stage. The 60% confluency was significantly lower than the 80% and 100% confluency (73.44%, 86.63%, and 87.17%, respectively, P < 0.05). The results indicate that the goat fibroblast cycle synchronization is the most effective at the serum concentration of 0.3%, 72 hours of synchronization and 100% confluency.
基金supported by“Mechanistic dissection of plant embryo and seed development“project(2014CB943401)from The National Basic Research Program,the Ministry of Science and Technology of China
文摘Although it is known that in most angiosperms mitosis in early endosperm development is syncytial and synchronized, it is unclear how the synchronization is regulated. We showed previously that APC11, also named ZYGI, in Arabidopsis activates zygote division by interaction and degradation of cyclin B1. Here, we report that the mutation in APC11/ZYG1 led to unsynchronized mitosis and over-accumulation of cyclin B1-GUS in the endosperm. Mutations in two other APC subunits showed similar defects. Transgenic expression of stable cyclin B1 in the endosperm also caused unsynchronized mitosis. Further, downregulation of APC11 generated multi-nucleate somatic cells with unsynchronized mitotic division. Together, our results suggest that APC/C-mediated cyclin B1 degradation is critical for cell cycle synchronization.
文摘同步作为小区搜索的重要部分,对小区的选择和响应时延等有着决定性的作用。在长期演进(Long Term Evolution,LTE)系统中主同步直接影响辅同步、全双工模式的识别,以至整个通信系统。结合主同步信号(Primary Synchronization Signal,PSS)良好的互相关性,给出了一种基于FFT快速相关的改进算法,采用重叠和分段联合检测的方式,并且通过数据循环后移保证序列完整性,来实现对半帧数据的快速搜索。理论分析和仿真表明,该算法能够有效降低计算量和同步时间。实验表明,该算法在LTE系统中能够准确高效地实现PSS序列的快速同步。
